Browse > Article
http://dx.doi.org/10.9721/KJFST.2020.52.1.26

Isolation and HPLC-DAD validation of xanthoangelol in Lespedeza bicolor extract  

Woo, Hyun Sim (Department of Forest Plant Industry, Baekdudaegan National Arboretum)
Kim, Yeong-Su (Department of Forest Plant Industry, Baekdudaegan National Arboretum)
Oh, Yu Jin (Department of Forest Plant Industry, Baekdudaegan National Arboretum)
Cho, Hae Jin (Department of Forest Plant Industry, Baekdudaegan National Arboretum)
Song, Se-Kyu (Department of Forest Plant Industry, Baekdudaegan National Arboretum)
Kim, Dae Wook (Department of Forest Plant Industry, Baekdudaegan National Arboretum)
Publication Information
Korean Journal of Food Science and Technology / v.52, no.1, 2020 , pp. 26-30 More about this Journal
Abstract
This study was undertaken to determine the characteristics of xanthoangelol, the major chalcone constituent derived from the extracts of different parts of Lespedeza bicolor. Xanthoangelol was isolated from the root extract using column chromatography and used as a standard for quantitative analysis. The structure of the isolated compound was established based on spectroscopic evidence. The HPLC-DAD method was validated for specificity, linearity, precision, accuracy, limit of detection, and limit of quantitation. The calibration curve of xanthoangelol had significant linearity (R2>0.9999). Limit of detection and limit of quantitation 0.018 and 0.059 ㎍/mL, respectively. The relative standard deviation values of precision test, and intra- and inter-day tests were less than 0.22 and 0.40%, respectively. In the recovery test, the accuracy ranged from 98.98-102.78% with RSD values less than 0.13%. The method validation parameters indicate the applicability of the HPLC method for quality control of food or drug formulations containing L. bicolor.
Keywords
Lespdeza bicolor; chalcone; xanthoangelol; HPLC-DAD; validation;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Sun W, Meng X, Lianq L, Jiang W, Huang Y, He J, Hu H, Almqvist J, Gao X, Wnag L. Molecular and Biochemical Analysis of Chalcone Synthase from Freesia hybrid in Flavonoid Biosynthetic Pathway. PLoS One 10(3): e0119054 (2015)   DOI
2 Sumiyoshi M, Taniguchib M, Babab K, Kimura Y. Antitumor and antimetastatic actions of xanthoangelol and 4-hydroxyderricin isolated from Angelica keiskei roots through the inhibited activation and differentiation of M2 macrophage. Phytomedicine 22: 759-767 (2015)   DOI
3 Tabata K, Motani N, Takayanagi N, Nishimura R, Asaml S, Kimura Y, Ukiya M, Hasegawa D, Akihisa T, Suzuk T. Xanthoangelol, a major chalcone constituent of Angelica keiskei, induces Apoptosis in Neuroblastoma and Leukemia Cells. Biol. Pharm. Bull. 28:1404-0407 (2005)   DOI
4 Ullah S. Methanolic extract from Lespedeza bicolor: potential candidates for natural antioxidant and anticancer agent, J. Tradit. Chin. Med. 37: 444-451 (2017)   DOI
5 Woo HS, Kim DW, Curis-Long MJ, Lee BW, Lee JH, Kim JY, Kang JE, Park KH. Potent inhibition of bacterial neuraminidase activity by pterocarpans isolated from the roots of Lespedeza bicolor, Bioorg. Med. Chem. Lett. 21: 6100-6103 (2011)   DOI
6 Akihisa T, Tokuda H, Ukiya M, Iizuka M, Schneider S, Ogasawara K, Mukainaka T, Iwatsuki K, Suzuki T, Nishino H. Chalcones, coumarins, and flavanones from the exudate of Angelica keiskei and their chemopreventive effects. Cancer Lett. 201: 133-137 (2003)   DOI
7 Do MH, Lee JH, Wahedi HM, Pak CH, Lee CH, Yeo EJ, Lim YS, Ha SK, Choi IW, Kim SY. Lespedeza bicolor ameliorates endothelial dysfunction induced by methylglyoxal glucotoxicity, Phytomedicine 36: 26-36 (2017)   DOI
8 ICH harmonized tripartite guideline, validation of analytical procedures: text and methodology Q2 (R1), ICH Harmonized Tripartite Guidelines, November 2005. http://www.ich.org/LOB/media/MEDIA417.pdf.
9 Kimura Y, Baba K. Antitumor and antimetastatic activities of Angelica keiskei roots, part 1: isolation of an active substance, xanthoangelol. Int. J. Cancer. 106: 429-437 (2003)   DOI
10 Korea National Arboretum. Available from: http://www.kna.go.kr. Accessed Jun. 1, 2012.
11 Lee JH, Jhoo JW. Antioxidant Activity of Different Parts of Lespedeza bicolor and Isolation of Antioxidant Compound. Korean J. Food Sci. Technol. 44: 763-771 (2012)   DOI
12 Lee SJ. Report on Korean folk-medicine. Seoul Natl Univ, Korea. pp. 75-101 (1972)
13 Lee YS, Chang, Park SC, Rim NR, Kim NW. Antioxidative and irritation response of Lespedeza bicolor. J. Toxicol. Public Health 21: 115-119 (2005a)
14 Lee SJ, Hossaine M.D.A., Park SC. A potential anti-inammation activity and depigmentation eect of Lespedeza bicolor extract and its fractions. Saudi J. Biol. Sci. 22: 9-14 (2016)
15 Lee YS, Joo EU, Kim NW. Antioxidant activity of extracts from the Lespedeza bicolor. Korean J. Food Preserv. 12: 75-79 (2005b)
16 Lee KI, Yang SA, Kim SM. Antioxidative and Nitric Oxide Production Inhibitory Activities of Lespedeza bicolor Stem Extracts Depending on Solvents. Korean J. Med. Crop Sci. 19: 368-372 (2011)   DOI
17 Li Y, Goto T, Ikutani1 R, Lin S, Takahashi N, Takahashi H, Jheng HF, Yu R, Taniguchi, Baba K, Murakami S, Kawada T. Xanthoangelol and 4-Hydroxyderrcin Suppress Obesity-Induced Inflammatory Responses. Obesity. 24: 2351-2360 (2016)   DOI
18 Maximov OB, Kulesh NI, Stepanenko LS, Dmitrenok PS. New prenylated isoflavanones and other constituents of Lespedeza bicolor. Fitoterapia. 75: 96-98. (2004)   DOI
19 Samiullah, Bano A, Girmay S, Tan G. Total Phenolic Content, Antioxidant, Antimicobial and Anticancer Activities of Lespedeza Bicolor Turcz (Papilionaceae). The International Society for Applied Life Sciences (ISALS). 10-12 (2012)
20 Ryu IS, Lee SJ, Lee SW, Mun YJ, Woo WH, Kim YM, Lee JC, Lim KS. Dermal Bioactive Properties of the Ethanol Extract from Flowers of Lespedeza bicolor. J. Korean Med. Ophthalmol. Otolaryngol. Dermatol. 20: 1-9 (2007)