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http://dx.doi.org/10.9721/KJFST.2019.51.6.565

Anti-inflammatory effects of seed ethanolic extracts of the common buckwheat and tartary buckwheat are mediated through the suppression of inducible nitric oxide synthase and pro-inflammatory cytokines in LPS-induced RAW 264.7 macrophage cells  

Kim, Su Jeong (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration)
Sohn, Hwang Bae (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration)
Lee, Kyung-Tae (Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung Hee University)
Shin, Ji-Sun (Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung Hee University)
Kim, Suyeon (Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung Hee University)
Nam, Jung Hwan (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration)
Hong, Su Young (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration)
Suh, Jong Taek (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration)
Chang, Dong Chil (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration)
Kim, Yul Ho (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration)
Publication Information
Korean Journal of Food Science and Technology / v.51, no.6, 2019 , pp. 565-575 More about this Journal
Abstract
The ethanolic seed extracts of the common buckwheat (CB) and tartary buckwheat (TB) were examined for their anti-oxidant and anti-inflammatory effects on lipopolysaccharide (LPS)-induced RAW 264.7 cells. In this study, it was observed that the rutin content of TB extracts was 65-78 times higher than the CB extracts, while quercetin was only detected in the TB extracts. In addition, TB extracts were observed to have 1.8-2.0 times higher flavonoid and polyphenolic content than the CB extracts. Cytotoxicity was not observed when both the buckwheat extracts were evaluated at concentrations in the range of 6.25-400 ㎍/mL. The treatment with TB extracts significantly suppressed the LPS-induced nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) expression at the protein and mRNA levels. The TB extracts more potently inhibited the LPS-induced production of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 than the CB extracts. The mRNA levels of TNF-α, IL-1β, and IL-6 were also significantly inhibited both by the TB and CB extracts in a pattern similar to their production.
Keywords
Anti-inflammatory; cytokinin; lipopolysaccharide; rutin; quercetin;
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Times Cited By KSCI : 7  (Citation Analysis)
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