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http://dx.doi.org/10.9721/KJFST.2017.49.4.453

A comparative study of the immuno-modulatory activities of ethanol extracts and crude polysaccharide fractions from Annona muricata L.  

Kim, Yi-Eun (Department of Food Science and Technology, Kongju National University)
Lee, Joeng-Hee (Teodam)
Sung, Nak-Yun (Department of Food Science and Technology, Kongju National University)
Ahn, Dong-Hyun (Department of Food Science&Technology/Institute of Food Science, Pukyong National University)
Byun, Eui-Hong (Department of Food Science and Technology, Kongju National University)
Publication Information
Korean Journal of Food Science and Technology / v.49, no.4, 2017 , pp. 453-458 More about this Journal
Abstract
This study compared the immuno-modulatory effects of ethanol extracts (A. muricata L. ethanol extracts, ALE) and crude polysaccharide fraction (A. muricata L. crude polysaccharide fraction, ALP) from Annona muricata L. in macrophages. Immuno-modulatory activity was determined by assessing cell viability, nitric oxide (NO) production, inducible NO synthase (iNOS) expression and cytokine production in RAW 264.7 a macrophage cell line. Both ALE and ALP treatment did not affect cytotoxicity, and ALP treatment significantly increased NO production. Additionally, cytokine production [tumor necrosis factor ($TNF-{\alpha}$; $2909.04{\pm}4.1pg/mL$), interleukin (IL)-6; $662.84{\pm}5.3pg/mL$, and $IL-1{\beta}$; $852.37{\pm}2.2pg/mL$), was highly increased in the ALP ($250{\mu}g/mL$) treated group compared to the ALE ($250{\mu}g/mL$) treated group ($TNF-{\alpha}$; $1564.50{\pm}6.1pg/mL$, IL-6; $517.24{\pm}4.1pg/mL$ and $IL-1{\beta}$; $237.23{\pm}1.8pg/mL$). Moreover, ALP treatment considerably increased the expression of mitogen-activated protein kinase (MAPKs) and nuclear $factor-{\kappa}B$ ($NF-{\kappa}B$) in the macrophages. Therefore, ALP can induce macrophage activation through MAPK and $NF-{\kappa}B$ signaling and this can be a potential candidate for development of nutraceuticals.
Keywords
Annona muricata leaves; crude polysaccharide fraction; immuno-modulatory activity; macrophage activation; nitric oxide production; cytokine production; mitogen-activated protein kinase;
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Times Cited By KSCI : 8  (Citation Analysis)
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