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http://dx.doi.org/10.9721/KJFST.2017.49.3.258

Comparison and validation of rutin and quercetin contents according to the extraction method of tartary Buckwheat (Fagopyrum tataricum Gaertn.)  

Kim, Su Jeong (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration)
Sohn, Hwang Bae (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration)
Kim, Geum Hee (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration)
Lee, Yu Young (Department of Central Area, National Institute of Crop Science, Rural Development Administration)
Hong, Su Young (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration)
Kim, Ki Deog (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration)
Nam, Jeong Hwan (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration)
Chang, Dong Chil (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration)
Suh, Jong Taek (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration)
Koo, Bon Cheol (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration)
Kim, Yul Ho (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration)
Publication Information
Korean Journal of Food Science and Technology / v.49, no.3, 2017 , pp. 258-264 More about this Journal
Abstract
The stability and accuracy of ultra-performance liquid chromatography (UPLC) used for evaluating the contents of rutin and quercetin in tartary buckwheat (Fagopyrum tataricum Gaertn.) seeds extracted by seven different extraction methods were determined. The seven extraction methods were reflux extraction (RE), ultra-sonification extraction (UE), stirrer extraction (SE), RE after UE (UE+RE), RE after SE (SE+RE), UE after SE (SE+UE), and RE with UE after SE (SE+UE+RE). Among the seven extraction methods used, RE yielded comparatively higher contents of rutin (2,277 mg/ 100 g) and quercetin (158 mg/100 g) than those yielded by other six extraction methods. The intra-day repeatability and inter-day precision of RE was 0.4-3.2% considering relative standard deviation (RSD), while accuracy was 88.8-102.4%. Therefore, RE with UPLC would be a rapid, accurate, and stable method for analyzing rutin and quercetin contents in tartary buckwheat.
Keywords
reflux extraction; rutin; quercetin; tartary buckwheat;
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Times Cited By KSCI : 11  (Citation Analysis)
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