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http://dx.doi.org/10.9721/KJFST.2016.48.5.502

Anti-inflammatory activities of fermented Rhus verniciflua stem bark extract and its growth inhibitory effect on Helicobacter pylori  

Choi, Eun Yeong (Applied Plant Sciences Program, Kangwon National University)
Suk, Ki Tae (Department of Internal Medicine, Hallym University College of Medicine)
Choi, Han Seok (Fermented Food Science Division, National Academy of Agricultural Science, RDA)
Kim, Myung Kon (Department of Food Science and Technology, Chonbuk National University)
Kwon, Yong Soo (Department of Pharmacy, Kangwon National University)
Kim, Myong Jo (Bioherb Research Institute, Kangwon National University)
Publication Information
Korean Journal of Food Science and Technology / v.48, no.5, 2016 , pp. 502-507 More about this Journal
Abstract
This study was designed to investigate the beneficial effects of fermented Rhus verniciflua stem bark extract (RVSBE) on the stomach. We evaluated RVSBE for its antimicrobial activity against Helicobacter pylori (H. pylori), along with its ability to reduce the viability of human gastric cancer AGS cells. In addition, its anti-inflammatory effect was examined by evaluating nitric oxide (NO) production, and inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 mRNA expression in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. RVSBE showed antimicrobial activity, as 2.0 mg of the extract produced a clear inhibition zone of 4.0 mm. RVSBE inhibited the growth of AGS cells by 20% at concentrations ranging from 0.25-1.0 mg/mL. Regarding the anti-inflammatory effects of RVSBE, at 0.1-1.0 mg/mL, the extract showed more than 75% inhibition of NO production. In addition, cells treated with 0.25 mg/mL RVSBE showed a 25% decrease in iNOS mRNA levels compared to those in the LPS-treated cells. These results suggest that RVSBE may have significant inhibitory effects on inflammatory mediators, and therefore, may be a potential anti-inflammatory candidate.
Keywords
fermented Rhus verniciflua; Helicobacter pylori; AGS cell; anti-inflammatory response;
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Times Cited By KSCI : 10  (Citation Analysis)
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