Browse > Article
http://dx.doi.org/10.9721/KJFST.2016.48.3.284

Protective Effect of Ethyl Acetate Fraction from Hibiscus Sabdariffa L. Extract against High Glucose-induced Oxidative Stress  

Seung, Tae Wan (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University)
Park, Sang Hyun (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University)
Park, Seon Kyeong (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University)
Ha, Jeong Su (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University)
Lee, Du Sang (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University)
Kang, Jin Yong (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University)
Kim, Jong Min (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University)
Lee, Uk (Division of Special Purpose Tree, National Institute of Forest Science)
Heo, Ho Jin (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University)
Publication Information
Korean Journal of Food Science and Technology / v.48, no.3, 2016 , pp. 284-288 More about this Journal
Abstract
To investigate the physiological effect of Hibiscus sabdariffa, in vitro antioxidant activities and neuroprotective effects against high glucose-induced oxidative stress were examined. The ethyl acetate fraction (EtOAc-Fr) from H. sabdariffa contained high total phenolic contents compared with other fractions but total anthocyanin contents were lower than 80% Ethanol extract showed the highest 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt radical scavenging activity and malondialdehyde inhibitory effect. Furthermore, the EtOAc-Fr decreased the intracellular reactive oxygen species level, and protected the neuron-like PC12 cells from high glucose-induced cytotoxicity. The EtOAc-Fr also presented inhibitory effects against acetylcholinesterase as an acetylcholine hydrolase enzyme. Finally, chlorogenic acids as main phenolics by high performance liquid chromatography analysis.
Keywords
high glucose; chlorogenic acid; Hibiscus sabdariffa L.;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Hunt JV, Dean RT, Wolff SP. Hydroxy radical production and autoxidative glycosylation. glucose autoxidation as the cause of protein damage in the experimental glycation model of diabetes mellitus and ageing. Biochem J. 256: 205-212 (1988)   DOI
2 Edwards JL, Vincent AM, Cheng HT, Feldman EL. Diabetic neuropathy: Mechanisms to management. Pharmacol. Therapeut. 120: 1-34 (2008)   DOI
3 Shen Y, Sheng R, Zhang J, He Q, Yang B, Hu Y. 2-Phenoxyindan-1-one derivatives as acetylcholinesterase inhibitors: A study on the importance of modifications at the side chain on the activity. Bioorgan. Med. Chem. 16: 7464-7653 (2008)
4 Ali BH, Wabel NA, Blunden G. Phytochemical, pharmacological and toxicological aspects of Hibiscus sabdariffa L.: A review. Phytother. Res. 19: 369-375 (2005)   DOI
5 Gradinaru G, Biliaderis CG, Kallithraka S, Kefalas P, Garcia-Viguera C. Thermal stability of Hibiscus sabdariffa L. anthocyanins in solution and in solid state: Effects of copigmentation and glass transition. Food Chem. 83: 423-436 (2003)   DOI
6 Kim HY, Woo KS, Hwang IG, Lee YR, Jeong HS. Effects of heat treatments on the antioxidant activities of fruits and vegetables. Korean J. Food Sci. Technol. 40: 166-170 (2008)
7 Jeong CH, Choi GN, Kim JH, Kwak JH, Kim DO, Kim YJ, Heo HJ. Antioxidant activities from the aerial parts of Platycodon grandiflorum. Food Chem. 118: 278-282 (2010)   DOI
8 Ghosh D, Konishi T. Anthocyanins and anthocyanin-rich extracts: role in diabetes and eye function. Asia Pac. J. Clin. Nutr. 16: 200-208 (2007)
9 Cheng GW, Patrick JB. Activity of phenylalanine ammonia-lyase (PAL) and concentrations of anthocyanins and phenolics in developing strawberry fruit. J. Am. Soc. Hortic. Sci. 116: 865-869 (1991)
10 Liu MH, Yuan C, He J, Tan TP, Wu SJ, Fu HY, Liu J, Yu S, Chen YD, Le QF, Tian W, Hu HJ, Zhang Y, Tian W. Resveratrol protects PC12 cells from high glucose-induced neurotoxicity via PI3K/Akt/FoxO3a pathway. Cell Mol. Neurobiol. 35: 513-522 (2015)   DOI
11 Ellman GL, Courtney KD, Andres Vjr, Featherstone RM. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem. Pharmacol. 4: 88-95 (1961)
12 Peng CH, Chyau CC, Chan KC, Chan TH, Wang CJ, Huang CN. Hibiscus sabdariffa polyphenolic extract inhibits hyperglycemia, hyperlipidemia, and glycation-oxidative stress while improving insulin resistance. J. Agr. Food Chem. 59: 9901-9909 (2011)   DOI
13 Kim EO, Lee YJ, Leem HH, Seo IH, Yu MH, Kang DH, Choi SW. Comparison of nutritional and functional constituents, and physicochemical characteristics of mulberrys from seven different Morus alba L. cultivars. J. Korean Soc. Food Sci. Nutr. 39: 1467-1475 (2010)   DOI
14 Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Bio. Med. 26: 1231-1237 (1999)   DOI
15 Kim HY, Yeo SI, Lee JT. Antioxidant effects of solvent reaction from Sanguisorbae officinalis L. with acetone. J. Appl. Biol. Chem. 54: 89-93 (2011)   DOI
16 Farah A, de Paulis T, Trugo LC, Martin PR. Effect of roasting on the formation of chlorogenic acid lactones in coffee. J. Agr. Food Chem. 53: 1505-1513 (2005)   DOI