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http://dx.doi.org/10.9721/KJFST.2015.47.4.525

Anti-adipogenic Effects of the Water Extracts of Defatted Green Tea Seed Cake  

Wee, Ji-Hyang (Food Research Center, Jeonnam Bioindustry Foundation)
Sung, Hea Mi (Food Research Center, Jeonnam Bioindustry Foundation)
Jung, Kyung Ok (Food Research Center, Jeonnam Bioindustry Foundation)
Kim, Suk Jung (Food Research Center, Jeonnam Bioindustry Foundation)
Shin, Yu-Rim (Biofood Story Co., Ltd.)
Park, Ju-Hyun (Biofood Story Co., Ltd.)
Kim, Jong-Deog (Research Center on Anti-Obesity and Health Care, Chonnam National University)
Publication Information
Korean Journal of Food Science and Technology / v.47, no.4, 2015 , pp. 525-533 More about this Journal
Abstract
The effect of the hot water extract of defatted green tea seed cake (GTSE) on lipid metabolism and the underlying mechanisms of lipolysis in mature 3T3-L1 adipocytes were investigated. In this study, we found that the naringenin content of GTSE was 5.5 mg/g; however, catechins were not detected. The intracellular lipid droplets were stained with Oil Red O dye and quantified. Compared to the control, lipid accumulation was significantly decreased by 52%, and intracellular triglyceride (TG) level was reduced by 33% after treatment with GTSE at a concentration of $40{\mu}g/mL$. To determine the mechanism of reduction in TG content, we determined the level of fatty acid synthase (FAS), phosphorylation of adenosine monophosphate-activated protein kinase (AMPK), and acetyl-coenzyme A carboxylase (ACC) in the cell model. Incubation of the 3T3-L1 adipocytes with GTSE stimulated AMPK and ACC phosphorylation in a dose-dependent manner, and decreased the expression of FAS.
Keywords
Defatted green tea seed cake; naringenin; 3T3-L1 adipocyte; lipolysis;
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Times Cited By KSCI : 5  (Citation Analysis)
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