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http://dx.doi.org/10.9721/KJFST.2013.45.4.515

Denaturing Gradient Gel Electrophoresis and Culture-based Analysis of the Bacterial Community in Cheonggukjang, a Korean Traditional Fermented Soybean Food from Gangwon Province  

Hong, Sung Wook (National Institute of Animal Science, RDA)
Lim, In Kyu (Division of Biological Science and Technology, Yonsei University)
Kim, Yong Woo (Division of Biological Science and Technology, Yonsei University)
Shin, Seung-Mee (Department Hotel Culinary and Catering Management, Chungwoon University)
Chung, Kun Sub (Division of Biological Science and Technology, Yonsei University)
Publication Information
Korean Journal of Food Science and Technology / v.45, no.4, 2013 , pp. 515-520 More about this Journal
Abstract
Bacterial communities derived from cheonggukjang and raw rice straw collected from a Mireuksan farm and a Heungup cheonggukjang in Gangwon province were investigated using both culture-based method and denaturing gradient gel electrophoresis (DGGE) analysis. Pure cultures, which were isolated from raw rice straw and cheonggukjang and cultured on tryptic soy agar plates (53-76 colonies per plate), were identified by analysis of 16S rRNA sequences. The traditional culture-based method and analysis of PCR-amplified 16S rRNA by DGGE revealed that for samples collected from the Mireuksan farm, Pantoea agglomerans and Bacillus subtilis were the predominant species in the raw rice-straw and cheonggukjang, respectively. For samples collected from the Heungup cheonggukjang, Bacillus amyloliquefaciens was the predominant species in both raw rice straw and cheonggukjang. Other microorganisms, including members of Pantoea, Bacillus, Enterococcus, Enterobacter, Pseudomonas, Rhodococcus, and Acinetobacter, were also present in the raw rice-straw and cheonggukjang, as were bacteria that could not be cultured.
Keywords
bacterial community; cheonggukjang; pure culture; denaturing gradient gel electrophoresis (DGGE);
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Times Cited By KSCI : 3  (Citation Analysis)
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1 Shon MY, Seo KI, Lee SW, Choi SH, Sung NJ. Biological activities of chungkugjang prepared with black bean and changes in phytoestrogen content during fermentation. Korean J. Food Sci. Technol. 32: 936-941 (2000)   과학기술학회마을
2 Shin ZI, Ahn CW, Nam HS, Lee HJ, Lee HJ, Moon TH. Fractionation of angiotensin converting enzyme (ACE) inhibitory peptides from soybean paste. Korean J. Food Sci. Technol. 27: 230- 234 (1995)   과학기술학회마을
3 Kwon HY, Kim YS, Kwon GS, Kwon CS, Sohn HY. Isolation of immuno stimulating strain Bacillus pumilus JB-1 from chungkook- jang and fermentational characteristics of JB-1. Kor. J. Microbiol. Biotechnol. 32: 291-296 (2004)   과학기술학회마을
4 Hong SS, Chung KS, Yoon KD, Cho YJ. Antimutagenic effect of solvent extracts of Korean fermented soybean products. Food Biotechnol. 5: 263-267 (1996)   과학기술학회마을
5 Benjamin H, Storkson J, Nagahara A, Pariza MW. Inhibition of benzopyrene-induced mouse forestomach neoplasia by dietary soy sauce. Cancer Res. 51: 2940-2942 (1991)
6 Shon MY, Seo KI, Park SK, Cho YS, Sung NJ. Some biological activities and isoflavone content of chungkuhjang prepared with black beans and Bacillus strain. J. Korean Soc. Food Sci. Nutr. 30: 662-667 (2001)   과학기술학회마을
7 Youn HK, Choi HS, Hur SH, Hong JH. Antimicrobial activities of viscous substance from chongkukjang fermented with different Bacillus sp. J. Fd. Hyg. Safety 16: 188-193 (2001)   과학기술학회마을
8 Kim YT, Kim WK, Oh HI. Screening and identification of the fibrinolytic bacterial strain from chungkook-jang. Kor. J. Appl. Microbiol. Biotechnol. 23: 1-5 (1995)
9 Kameda Y, Oira S, Matsui K, Kanatomo S, Hase T. Antitumor activity of Bacillus natto. V. Isolation and characterization of surfactin in the culture medium of Bacillus natto KMD 2311. Chem. Pharm. Bull. 22: 938-994 (1974)   DOI   ScienceOn
10 Kim YS, Jung HJ, Park YS, Yu TS. Characteristics of flavor and functionality of Bacillus subtilis K-20 chungkukjang. Korean J. Food Sci. Technol. 35: 475-478 (2003)   과학기술학회마을
11 Lee MY, Park SY, Jung KO, Park KY, Kim SD. Quality and functional characteristics of chungkukjang prepared with various Bacillus sp. isolated from traditional chungkukjang. J. Food Sci. 70: M191-M196 (2005)
12 Kwon HY, Kim YS, Kwon GS, Kwon CS, Sohn HY. Isolation of immuno stimulating strain Bacillus pumilus JB-1 from chungkook- jang and fermentational characteristics of JB-1. Kor. J. Microbiol. Biotechnol. 32: 291-296 (2004)   과학기술학회마을
13 Kim SS, Lee JH, Ahn YS, Kim JH, Kang DK. A fibrinolytic enzyme from Bacillus amyloliquefaciens D4-7 isolated from chungkookjang; It's characterization and influence of additives on thermostability. Kor. J. Microbiol. Biotechnol. 31: 271-276 (2003)
14 Kwon GH, Lee HA, Park JY, Kim JS, Lim JK, Park CS, Kwon DY, Kim YS, Kim JH. Development of a RAPD-PCR method for identification of Bacillus species isolated from cheonggukjang. Int. J. Food Microbiol. 129: 282-287 (2009)   DOI   ScienceOn
15 Farnleitner AH, Zibuschka F, Burtscher MM, Lindner G, Reischer G, Mach RL. Eubacterial 16S-rDNA amplicon profiling: a rapid technique for comparsion and differentiation of heterotrophic plate count communities from drinking water. Int. J. Food Microbiol. 92: 333-375 (2004)   DOI   ScienceOn
16 Fischer SG, Lerman LS. DNA fragments differing by single basepair substitutions are separated in denaturing gradient gels: correspondence with melting theory. P. Natl. Acad. Sci. USA 80: 1579-1583 (1983)   DOI   ScienceOn
17 Walter J, Tannock GW, Tilsala-Timisjarvi A, Rodtong S, Loach DM, Munro K, Alatossava T. Detection and identification of gastrointestinal Lactobacillus species by using denaturing gradient gel electrophoresis and species specific PCR primers. Appl. Environ. Microb. 66: 297-303 (2000)   DOI   ScienceOn
18 Watanabe T, Asakawa S, Nakamura A, Nagaoka K, Kimura M. DGGE method for analyzing 16S rDNA of methanogenic archaeal community in paddy field soil. FEMS Microbiol. Lett. 232: 153-163 (2004)   DOI   ScienceOn
19 Kim MN, Bang HJ. Comparison of culture-dependent and DGGE based methods the analysis of marine bacterial community. Korean J. Environ. Biol. 24: 307-313 (2006)