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http://dx.doi.org/10.9721/KJFST.2013.45.4.508

T-lymphocyte Inactivation and Anti-atopic Effects of Diarylheptanoid Hirsutenone Isolated from Alnus japonica  

Lee, Do Ik (Department of Immunology, College of Pharmacy, Chung-Ang University)
Seo, Seong Jun (Department of Dermatology, College of Medicine, Chung-Ang University)
Joo, Seong Soo (Department of Marine Molecular Biotechnology, College of Life Science, Gangneung-Wonju National University)
Publication Information
Korean Journal of Food Science and Technology / v.45, no.4, 2013 , pp. 508-514 More about this Journal
Abstract
2Department of Marine Molecular Biotechnology, College of Life Science, Gangneung-Wonju National University Recently, we reported that diarylheptanoid hirsutenone (HST) effectively inactivated T lymphocytes. However, it has not been evaluated whether HST is involved in calcineurin or calmodulin inactivation. In the present study, cells were treated with T-cell inhibitors with or without HST. Our results revealed that HST successfully inhibited expression of T-helper type I (Th1) and Th2 cytokines. Co-treatment with HST and nuclear factor-activated T cell (NFAT) activation inhibitor III (INCA-6) showed a more sensitive effect than that with other inhibitors, suggesting that HST contributes to inhibition of dephosphorylation of NFAT in the cytosol. HST up-regulated cell cycle arrest genes and inhibited the growth of Staphylococcus aureus. These effects were confirmed in an NFAT electrophoretic-mobility shift assay via successful inhibition of NFAT translocation and in the histological recovery in a 2,4-dinitrochloro benzene-induced in vivo model. Taken together, HST was shown to effectively inhibit T-cell activation via inhibition of cytosolic NFAT dephosphorylation, similar to INCA-6.
Keywords
diarylheptanoid hirsutenone; calcineurin; calmodulin; T lymphocyte; nuclear factor-activated T cell;
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