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http://dx.doi.org/10.9721/KJFST.2012.44.4.452

Resveratrol Induces Apoptosis through PI3K/Akt and p53 Signal Pathway in MDA-MB-231 Breast Cancer Cells  

Kwon, Jung-Ki (Department of Companion and Laboratory Animal Science, Kongju National University)
Park, Young-Seok (Department of Companion and Laboratory Animal Science, Kongju National University)
Park, Byung-Kwon (Department of Companion and Laboratory Animal Science, Kongju National University)
Kim, Byeong-Soo (Department of Companion and Laboratory Animal Science, Kongju National University)
Kim, Sang-Ki (Department of Companion and Laboratory Animal Science, Kongju National University)
Jung, Ji-Youn (Department of Companion and Laboratory Animal Science, Kongju National University)
Publication Information
Korean Journal of Food Science and Technology / v.44, no.4, 2012 , pp. 452-459 More about this Journal
Abstract
This study was conducted in order to investigate the effect of resveratrol on the induction of apoptosis in MDA-MB-231 breast cancer cells. The result of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl terazolium bromide (MTT) assay shows that cell viability significantly decreased in a dose and time-dependent manner. 4',6-diamidino-2-phenylindole (DAPI) staining shows significantly increased chromatin condensation in a dose and time-dependent manner. Resveratrol increased the expression of p53, cleaved-caspase-3, and cleaved-caspase-9, whereas the expression of PI3K/Akt decreased in a time-dependent manner. We investigated the in vivo tumor growth inhibitory effect of resveratrol. Tumor volume was significantly decreased in the 50 mg/kg resveratrol-administration group compared to the control group. In the 50 mg/kg treated group. Apoptosis cells were frequently observed by terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) assay. Immunohistochemistry staining shows increased the expression of p53, cytochrome-C, and cleaved-caspase-3 in the 50 mg/kg treated group. These results indicate that resveratrol induced apoptosis through PI3K/Akt and p53 signal pathway in MDA-MB-231 cell.
Keywords
resveratrol; apoptosis; PI3K/Akt; breast cancer cell; p53;
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Times Cited By KSCI : 7  (Citation Analysis)
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