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http://dx.doi.org/10.9721/KJFST.2012.44.3.343

Immuno-stimulating Activities of Mannose-rich Polysaccharides Isolated from Korean Black Raspberry Wine  

Lee, Ho (Department of Food Science & Biotechnology, Kyonggi University)
Publication Information
Korean Journal of Food Science and Technology / v.44, no.3, 2012 , pp. 343-349 More about this Journal
Abstract
Polysaccharides isolated from Korean black raspberry wine were examined for their chemical properties and immuno-modulating activities. The molecular mass of RB-1b-I, the main polysaccharide in black raspberry wine, was estimated as 180 kDa and it contained a significant proportion of mannose (76.8%) and 4 different minor component sugars such as galactose (15.8%), arabinose (3.8%), glucose (2.6%) and rhamnose (1.2%). This indicated that RB-1b-I was mainly present as a mannan, which had originated from the cell walls of fermenting yeasts. On the other hand, RB-1b-I induced high levels of macrophage activation as well as mitogenicity regarding murine splenocytes in vitro. The intravenous administration of RB-1b-I significantly augmented NK cytotoxicity against YAC-1 tumor cells. RB-1b-I also showed potent anti-complementary activity in a dose-dependent manner via both alternative and classical pathways. Results indicated that Korean black raspberry wine contains peculiar polysaccharides which provide beneficial immuno-stimulating activities for human health.
Keywords
raspberry wine; polysaccharide; mannan; immuno-stimulating activity;
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1 Nathan CF, Murray HW, Cohen ZA. Current concepts: The macrophage as an effector cell. New Engl. J. Med. 303: 662-665 (1980)   DOI
2 Shida K, Suzuki T, Kiyoshima-Shibata J, Shimada S, Nanno M. Essential roles of monocytes in stimulating human peripheral blood mononuclear cells with Lactobacillus casei to produce cytokines and augment natural killer cell activity. Clin. Vaccine Immunol. 13: 997-1003 (2006)   DOI
3 Hunter CA, Chizzonite R, Remington JS. IL-1 beta is required for IL-12 to induce production of IFN-gamma by NK cells. A role for IL-1 beta in the T cell-independent mechanism of resistance against intracellular pathogens. J. Immunol. 155: 4347-4354 (1995)
4 Saito H, Tomioka H, Sato K. PSK, a polysaccharide from Coriolus versicolor, enhances oxygen metabolism of murine peritoneal macrophages and the host resistance to listerial infection. J. Gen. Microbiol. 134: 1029-1035 (1988)
5 Kwon MH, Sung HJ. Characteristics of immune response by polysaccharides with complement system activity. Food Sci. Indus. 30: 30-43 (1997)
6 Jung YJ, Chun H, Kim KI, An JH, Shin DH, Hong BS, Cho HY, Yang HC. Purified polysaccharide activating the complement system from leaves of Diospyos kaki L. Korean J. Food Sci. Technol. 34: 879-884 (2002)   과학기술학회마을
7 Whaley K. The complement system. pp. 1-35. In: Complement in Health and Disease. Whaley K (ed). MTP Press, Lancaster, PA, USA (1986)
8 Kim JH, Shin KS, Lee H. Characterization and action mode of anti-complementary substance prepared from Lactobacillus plantarum. Korean J. Food Sci. Technol. 34: 290-295 (2002)   과학기술학회마을
9 Hudson L, Hay FC. Two dimensional or crossed immunoelectrophoresis. pp. 244-246. In: Practical Immunology. Blackwell Scientific Publications, Oxford, England (1989)
10 Wang SY, Lin HS. Antioxidant activity in fruits and leaves of blackberry, raspberry, and strawberry varies with cultivar and developmental stage. J. Agr. Food Chem. 48: 140-146 (2000)   DOI
11 Wang SY, Jiao H. Scavenging capacity of berry crops on superoxide radicals, hydrogen peroxide, hydroxyl radicals, and singlet oxygen. J. Agr. Food Chem. 48: 5677-5684 (2000)   DOI
12 Seeram NP, Adams LS, Zhang Y, Lee R, Sand D, Scheuller HS, Heber D. Blackberry, black raspberry, blueberry, cranberry, red raspberry, and strawberry extracts inhibit growth and stimulated apoptosis of human cancer cells in vitro. J. Agr. Food Chem. 54: 9329-9339 (2006)   DOI
13 Chen T, Hwang HJ, Rose ME, Nines RG, Stoner GD. Chemopreventive properties of black raspberries in N-nitrosomethylbenzylamine-induced rat esophageal tumorigenesis: Down-regulation of cyclooxygenase-2, inducible nitric oxide synthase, and c-Jun. Cancer Res. 66: 2853-2859 (2006)   DOI
14 Jung JW, Son MY, Jung SW, Nam PW, Sung JS, Lee SJ, Lee KG. Antioxidant properties of Korean black raspberry wines and their apoptotic effects on cancer cells. J. Sci. Food Agr. 89: 970-977 (2009)   DOI
15 Lee SJ, Ahn B. Changes in physicochemical characteristics of black raspberry wines from different regions during fermentation. Korean J. Food Sci. Technol. 41: 662-667 (2009)   과학기술학회마을
16 Cho JY, Kim SJ, Lee HJ, Kim JY, Lym IJ, Kang SK, Park KH, Moon JH. Isolation and identification of low molecular volatile compounds from ethyl acetate layer of Korean black raspberry (Rubus coreanus Miq.) wine. Korean J. Food Sci. Technol. 43: 558-563 (2011)   과학기술학회마을   DOI
17 Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. Colorimetric method for determination of sugars and related substances. Anal. Chem. 28: 350-356 (1956)   DOI
18 Jones TM, Albersheim P. A gas chromatography method for the determination of aldose and uronic acid constituents of plant cell wall polysaccharide. Plant Physiol. 49: 926-936 (1972)   DOI
19 Blumenkrantz N, Asboe-Hansen G. New method for quantitative determination of uronic acid. Anal. Biochem. 54: 484-489 (1973)   DOI
20 Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254 (1976)   DOI
21 Suzuki I, Tanaka H, Kinoshita A, Oikawa S, Osawa M, Yadomae T. Effects of orally administered ß-glucan on macrophage function in mice. Int. J. Immunopharmacol. 12: 675-684 (1990)   DOI
22 Miyagawa S, Hirose H, Shirakura R, Naka Y, Nakata S, Kawashima Y, Seya T, Matsumoto M, Uenaka A, Kitamura H. The mechanism of discordant xenograft rejection. Transplantation 46: 825-830 (1995)
23 Kabat EA, Mayer MM. Complement and complement fixation. pp. 133-240. In: Experimental Immunochemistry. 2nd ed. Charles C (ed). Thomas Publisher, IL, USA (1971)
24 Shimura K, Ito H, Hibasami H. Screening of host-mediated antitumor polysaccharides by crossed immunoelectrophoresis using fresh human serum. Jpn. J. Pharmacol. 33: 403-408 (1983)   DOI
25 Wollin SD, Jones PJH. Alcohol, red wine, and cardiovascular disease. J. Nutr. 131: 1401-1404 (2001)   DOI
26 Estruch R. Wine and cardiovascular disease. Food Res. Int. 33: 219-226 (2000)   DOI
27 Kerry NL, Abbey M. Red wine and fractionated phenolic compounds prepared from red wine inhibit low density lipoprotein oxidation in vitro. Atherosclerosis 135: 93-102 (1997)   DOI
28 Fuhrman B, Lavy A, Aviram M. Consumption of red wine with meals reduces the susceptibility of human plasma and low-density lipoprotein to lipid peroxidation. Am. J. Clin. Nutr. 61: 549-554 (1995)   DOI
29 Tedesco I, Russo M, Russo P, Iacomino G, Russo GL, Carraturo A, Faruolo C, Moio L, Palumbo R. Antioxidant effect of red wine polyphenols on red blood cells. J. Nutr. Biochem. 11: 114-119 (2000)   DOI
30 Whitehead TP, Robinson D, Allaway S, Syms J, Hale A. 'Effect of red wine ingestion on the antioxidant capacity of serum. Clin. Chem. 41: 32-35 (1995)
31 Hager A, Howard LR, Prior RL, Brownmiller C. Processing and storage effects on monomeric anthocyanins, percent polymeric color, and antioxidant capacity of processed black raspberry products. J. Food Sci. 73: 134-140 (2008)
32 Seeram NP. Berry fruits: Compositional elements, biochemical activities, and the impact of their intake on human health, performance, and disease. J. Agr. Food Chem. 56: 627-629 (2008)   DOI
33 Tulio AZ Jr, Reese RN, Wyzogoski FJ, Rinaldi PL, Fu R, Scheerens JC, Miller AR. Cyanidin 3-rutinoside and cyanidin 3-xylosylrutinoside as primary phenolic antioxidants in black raspberry. J. Agr. Food Chem. 56: 1880-1888 (2008)   DOI
34 Ishii T. O-Acetylated oligosaccharides from pectins of potato tuber cell walls. Plant Physiol. 113: 1265-1272 (1997)   DOI
35 Klis FM, Mol P, Hellingwerf K, Brul S. Dynamics of cell wall structure in Saccharomyces cerevisiae. FEMS Microbiol. Rev. 26: 239-256 (2002)   DOI
36 Ridley BL, O'Neill MA, Mohnen D. Pectin: Structure, biosynthesis, and oligogalcturonide-related signaling. Phytochemistry 57: 929-67 (2001)   DOI
37 O'Neill M, Albersheim P, Darvill A. The pectic polysaccharides of primary cell walls. Vol. 2, pp. 415-441. In: Methods In Plant Biochemistry. Carbohydrates. Dey PM (ed). Academic, London, England (1990)
38 Engelsen SB, Cros S, Mackie W, Perez S. A molecular builder for carbohydrates: Application to polysaccharides and complex carbohydrates. Biopolymers 39: 417-433 (1996)   DOI
39 Male D, Brostoff J, Roth DB, Roitt I. Cells, tissues, and organs of the immune system. pp. 19-58. In: Immunology. 7th ed. Mosby, St. Louis, MO, USA (2006)
40 Keller R, Keist R, Wechsler A, Leist TP, van der Meide PH. Mechanism of macrophage-mediated tumor cell killing: A comparative analysis of the roles of reactive nitrogen intermediates and tumor necrosis factor. Int. J. Cancer 46: 682-686 (1990)   DOI