Browse > Article
http://dx.doi.org/10.9721/KJFST.2012.44.3.293

Analysis of Residual Fluoroquinolones Contents in Fish  

Park, Young-Ae (Gangnam Agro-marine Products Inspection Center, Seoul Metropolitan Government Research Institute of Public Health and Environment)
Yuk, Dong-Hyun (Gangnam Agro-marine Products Inspection Center, Seoul Metropolitan Government Research Institute of Public Health and Environment)
Kim, Su-Un (Gangnam Agro-marine Products Inspection Center, Seoul Metropolitan Government Research Institute of Public Health and Environment)
Kim, Jin-Ah (Gangnam Agro-marine Products Inspection Center, Seoul Metropolitan Government Research Institute of Public Health and Environment)
Park, Ae-Sook (Gangnam Agro-marine Products Inspection Center, Seoul Metropolitan Government Research Institute of Public Health and Environment)
Kim, Youn-Cheon (Gangnam Agro-marine Products Inspection Center, Seoul Metropolitan Government Research Institute of Public Health and Environment)
Kim, Moo-Sang (Gangnam Agro-marine Products Inspection Center, Seoul Metropolitan Government Research Institute of Public Health and Environment)
Publication Information
Korean Journal of Food Science and Technology / v.44, no.3, 2012 , pp. 293-299 More about this Journal
Abstract
The residual contents of fluoroquinolones in fish and shrimp were analyzed by using HPLC-FLD and LC-MS. The limit of detection (LOD) and limit of quantification (LOQ) for fluoroquinolones by HPLC-FLD were 0.0030-0.0083 and 0.0090-0.0252 mg/kg, respectively. Those by LC-MS were 0.0019-0.0040 and 0.0059-0.0122 mg/kg, respectively. Recoveries regarding ofloxacin, norfloxacin, pefloxacin, ciprofloxacin and enrofloxacin ranged from 76.4-104.6%. The detection rate of fluoroquinolones was 7.5% from a total of 268 samples. In only one Korean bullhead sample, the sum of ciprofloxacin and enrofloxacin contents was detected at a higher level than its maximum residue limits (MRL) of 0.1 mg/kg. Ofloxacin, norfloxacin and pefloxacin were not detected in any samples and detection rates of ciprofloxacin and enrofloxacin were 3.4% and 6.7%, respectively. In detection rate by species eel (2.6%), loach (2.2%), Korean bullhead (1.5%), carp (0.7%) and snakehead (0.4%) were in order.
Keywords
fluoroquinolones; HPLC-FLD; LC-MS;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Cun L, Zhanhui W, Xingyuan C, Ross CB, Suxia Z, Shuangyang D, Xiaowei L, Jianzhong S. Development of an immunoaffinity column method using broad-specificity monoclonal antibodies for simultaneous extraction and cleanup of quinolone and sulfona-mide antibiotics in animal muscle tissues. J. Chromatogr. A 1209: 1-9 (2008)   DOI
2 Kim HY, Shin MS, Choi HJ, Park SJ, Song JS, Cheong SY, Choi SH, Lee HJ, Kim YS, Choi JC. Analysis of fluoroquinolone antibiotics in foods. Korean J. Food Sci. Technol. 41: 636-643 (2009)   과학기술학회마을
3 Kim HY, Chung SY, Choi SH, Lee JS, Choi IS, Cho MJ, Shin MS, Song JS, Choi JC, Park HO, Ha SC, Shin IS, Seo EC. Monitoring of veterinary drug residues in foods produced in Korea. Korean J. Food Sci. Technol. 42: 653-663 (2010)   과학기술학회마을
4 KFDA. Korea Food Code. Korea Food and Drug Administration, Seoul, Korea. pp. 10-5-82 (2009)
5 Roger C. Validation of chromatographic methods in biomedical analysis viewpoint and discussion. J. Chromatogr. A 689: 175- 180 (1997)   DOI
6 Wolfson JS, Hooper DC. The fluoroquinolones: Structures, mechanism of action, and resistance and spectra of activity in vitro. Antimicrob. Agents Ch. 28: 581-586 (1985)   DOI
7 Blondeau JM. Fluoroquinolones: Mechanism of action classification and development of resistance. Surv. Ophthalmol. 49: S73-S78 (2004)   DOI
8 Koga H, Itoh A, Murayama S, Suzue S, Irikura T. Structure activity relationships of antibacterial 6,7-and 7,8-distributed 1-alkyl-1,4-dihydro-4-oxoquinoline-3-carboxylic acids. J. Med. Chem. 23: 1358-1363 (1980)   DOI
9 Kasuga Y, Sugitani A, Yamada F, Arai M, Morikawa S. Oxolinic acid residues in tissues of cultured rainbow trout and ayu fish. J. Food Hyg. Soc. Jpn. 25: 512-615 (1984)   DOI
10 Herikstad H, Hayers P, Mokhtar M, Fracaro ML, Threlfall EJ, Angulo FJ. Emerging quinolone-resistant Salmonella in the USA. Emerg. Infect. Dis. 3: 371-372 (1997)   DOI
11 Smith KE, Besser JM, Hedberg CW, Leano FT, Bender JB, Wicklund JH, Johnson BP, Moore KA, Osterholm MT. Quinolone-resistant Campylobacter jejuni infections in Minnesota. New Engl. J. Med. 340: 1525-1532 (2003)
12 Choi YH, Kim YJ, Lee KH, Kang YI, Lee JH. Determination and survey of fluoroquinolones in meats and eggs. Korean J. Vet. Serv. 32: 281-286 (2009)   과학기술학회마을
13 Choi YJ, Yun IR, Nam SY, Park YH, Kim BH, Son SG. Study on analytical method of fluoroquinolone residues in eggs by LC/MS/MS. Korean J. Vet. Serv. 30: 13-21 (2007)
14 Ralph K, Franz JH, Frank R. Simple and rapid determination of enrofloxacin and ciprofloxacin in edible tissues by turbulent flow chromatography/tandem mass spectrometry(TFC-MS/MS). Anal. Chim. Acta 637: 208-213 (2009)   DOI