Browse > Article
http://dx.doi.org/10.9721/KJFST.2012.44.2.173

Preparation and Characterization of Urushiol Free Fermented Rhus verniciflua Stem Bark (FRVSB) Extracts  

Choi, Han-Seok (Fermentation and Food Processing Division, National Academy of Agricultural Science, RDA)
Yeo, Soo-Hwan (Fermentation and Food Processing Division, National Academy of Agricultural Science, RDA)
Jeong, Seok-Tae (Fermentation and Food Processing Division, National Academy of Agricultural Science, RDA)
Choi, Ji-Ho (Fermentation and Food Processing Division, National Academy of Agricultural Science, RDA)
Park, Hyo-Suk (Department of Agricultural Chemistry, Wonkwang University)
Kim, Myung-Kon (Department of Food Science and Technology, Chonbuk National University)
Publication Information
Korean Journal of Food Science and Technology / v.44, no.2, 2012 , pp. 173-178 More about this Journal
Abstract
The water extract of the fermented $Rhus$ $verniciflua$ stem bark (FRVSB) was prepared by hot water extracting at $100^{\circ}C$ for 8 h. The urushiol content of the FRVSB water extract was determined by HPLC. The urushiol was not contained in FRVSB water extract, whereas $Rhus$ $verniciflua$ stem bark (RVSB) water extract contained 3.4 mg%. At the lab scale size, suitable water extraction condition for a total solid, polyphenol and flavonoid from FRVSB was at over $100^{\circ}C$ for 6-8 h. The total solid contents was reduced in pilot scale processing system, with 5.7% of the extraction yield. The proximate composition (%) of FRVSB water extract obtained from industrial installation was moisture 4.34, crude fat 1.69, crude protein 10.21, and crude ash 15.80. Gallic acid (1,090.5 mg%) was the most abundant compound in phenolic acids, while fisetin (135.7 mg%) was the predominant flavonoid. The free sugar content was mannitol of 3.48%, glycerol of 0.19%, and glucose of 0.19%. Alanine (244 ppm), serine (231 ppm), and leucine (218 ppm) were predominant amino acids.
Keywords
urushiol; Rhus verniciflua; FRVSB; preparation; physicochemical properties;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
연도 인용수 순위
1 Choi HS, Kim MK, Park HS, Yun SE, Mun SP, Kim JS, Sapkota K, Kim S, Kim TY, Kim SJ. Biological detoxification of lacquer tree (Rhus verniciflua Stokes) stem bark by mushroom species. Food Sci. Biotechnol. 16: 935-942 (2007)
2 Moreno MIN, Isla MI, Sampietro AR, Vattuone MA. Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. J. Ethnopharmacol. 71: 109-114 (2000)   DOI
3 KFDA. Analytical Methods of Korean Food Code. Korea Food & Drug Administration, Cheongwon, Korea (2010)
4 Draper WM, Wijekoon D, Makinney M, Behniwal P, Perera SK, Flessel CP. Atmospheric pressure ionization LC-MS-MS determination of urushiol congeners. J. Agr. Food Chem. 50: 1852-1858 (2002)   DOI
5 Kim MJ, Hyun JO. Genetic variation in urushiol components of Rhus verniciflua Stokes. Korean J. Breed. 29: 115-123 (1997)
6 Korea Forest Service. Information of forest. Available from: http:/ /www.forest.go.kr/kfsweb/search2/index.jsp?kwd=%EC%98%BB& pageNum=1&category=KNOWLEDGE&reSearchFlg=false&subcategory=& sortName=&tagFlg=&sitepageNum=1&preKwd= %EC%98%BB. Accessed Nov. 11, 2011.
7 Choi HS, Kim BH, Yeo SH, Jeong ST, Choi JH, Park HS, Kim MK. Physicochemical properties and physiological activities of Rhus verniciflua Stem Bark Cultured with Fomitella fraxinea. Korean J. Mycol. 38: 172-178 (2010)   과학기술학회마을   DOI
8 Buswell JA, Cai YJ, Chang ST. Fungal and substrate associated factors affecting the ability of individual mushroom species to utilize different lignocellulosic growth substrates. pp. 141-150. In: Mushroom Biology and Mushroom Products. Chang ST, John AB, Chiu SW (eds). The Chinese University Press, Hong Kong, China (1993)
9 Jang HS, Kook SH, Son YO, Kim JG, Jeon YM, Jang YS, Choi KC, Kim J, Han SK, Lee KY, Park BK, Cho NP, Lee JC. Flavonoids purified from Rhus verniciflua Stokes actively inhibit cell growth and induce apoptosis in human osteosarcoma cells. Biochim. Biophys. Acta 1726: 309-316 (2005)   DOI
10 Lee JC, Lee KY, Kim J, Na CS, Jung NC, Chung GH, Jang YS. Extract from Rhus verniciflua Stokes is capable of inhibiting the growth of human lymphoma cells. Food Chem. Toxicol. 42: 1383-1388 (2004)   DOI
11 Kim SG, Rhyu DY, Kim DK, Ko DH, Kim YK, Lee YM, Jung HJ. Inhibitory effect of heartwood of Rhus verniciflua Stokes on lipid accumulation in 3T3-L1 cells. Korean J. Pharmacogn. 41: 21-25 (2010)   과학기술학회마을
12 Jeon WK, Lee JH, Kim HK, Lee AY, Lee SO, Kim YS, Ryu SY, Kim SY, Lee YJ, Ko BS. Anti-platelet effects of bioactive compounds isolated from the bark of Rhus verniciflua Stokes. J. Ethnopharmacol. 106: 62-69 (2006)   DOI
13 Kim MO, Kim JS, Sa YJ, Jeong HJ, Chun WJ, Kwon YS, Kim TY, Choi HS, Yu CY, Kim MJ. Screening of extraction solvent condition of fermented Rhus verniciflua stem bark by antioxidant activities. Korean J. Medicinal Crop Sci. 18: 217-223 (2010)   과학기술학회마을
14 Jung NC. Biological activity of urushiol and flavonoids from Lac tree (Rhus verniciflua Stokes). PhD thesis, Chonnam National University, Gwangju, Korea (1998)
15 Son YO, Lee KY, Lee JC, Jang HS, Kim JG, Jeon YM, Jang YS. Selective antiproliferative and apoptotic effects of flavonoids purified from Rhus verniciflua Stokes on normal versus transformed hepatic cell lines. Toxicol. Lett. 135: 115-125 (2005)
16 Yun SK, Ko KB, Song IM, Choi SP, Ihm CW. Epidemiologic study on systemic contact dermatitis due to ingestion of Rhus. Korean J. Dermatol. 40: 253-257 (2002)   과학기술학회마을
17 Kim JB. Identification of antioxidative component from stem bark of Rhus verniciflua. Korean J. Food Nutr. 16: 60-65 (2003)   과학기술학회마을
18 Lee JC, Kim J, Lim, KT, Jang YS. Identification of Rhus verniciflua Stokes compounds that exhibit free radical scavenging and anti-apoptotic properties. Biochim. Biophys. Acta 1570: 181-191 (2002)   DOI
19 Park KY, Jung GO, Lee KT, Choi JW, Choi MY, Kim GT, Jung JJ, Park HJ. Antimutagenic activity of flavonoids from the heartwood of Rhus verniciflua. J. Ethnopharmacol. 90: 73-79 (2004)   DOI
20 Lee JD, Huh JE, Jeon GS, Yang HR, Woo HS, Choi DY, Park DS. Flavonol-rich RVHxR from Rhus verniciflua Stokes and its major compound fisetin inhibits inflammation-related cytokines and angiogenic factor in rheumatoid arthritic fibroblast-like synovial cells and in vivo models. Int. Immunopharmacol. 9: 268- 276 (2009)   DOI
21 Lee DS, Jeong GS, Li B, Park H, Kim YC. Anti-inflammatory effects of sulfuretin from Rhus verniciflua Stokes via the induction of heme oxygenase-1 expression in murine macrophages. Int. Immunopharmacol. 10: 850-858 (2010)   DOI
22 Kim JS, Kwon YS, Chun WJ, Kim TY, Sun J, Yu CY, Kim MJ. Rhus verniciflua Stokes flavonoid extracts have anti-oxidant, antimicrobial and $\alpha$-glucosidase inhibitory effect. Food Chem. 120: 539-543 (2010)   DOI
23 Kim BH. The effect of irradiation and peroxyacetic acid treatment on the reduction of urushiol of Rhus verniciflua Stokes and the physiological activities. MS thesis, Chonbuk National University, Gwangju, Korea (2010)
24 KFS. 2007 the investigation of forestry management of Korea. Korea Forest Service, Daejeon, Korea (2008)
25 Choi WC, Park SJ, Kwon SP. Process for the preparation of Rhus verniciflua extract having excellent anti-cancer activity and anticancer pharmaceutical composition containing same. Int. Patent WO/2007/049846 (2007)