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http://dx.doi.org/10.9721/KJFST.2012.44.1.082

Effects of Chrysanthemum indicum L. Extract on the Function of Osteoblastic MC3T3-E1 Cells under Oxidative Stress Induced by Hydrogen PeroxideJee  

Yun, Jee-Hye (Department of Food and Nutrition, Duksung Women's University)
Hwang, Eun-Sun (Department of Nutrition and Culinary Science, Hankyong National University)
Kim, Gun-Hee (Department of Food and Nutrition, Duksung Women's University)
Publication Information
Korean Journal of Food Science and Technology / v.44, no.1, 2012 , pp. 82-88 More about this Journal
Abstract
Chrysanthemum indicum L. (Asteraceae) is a traditional herbal medicine that has been used for the treatment of inflammation, hypertension, and respiratory diseases due to its strong antagonistic activity against inflammatory cytokines. The effects of Chrysanthemum indicum L. Extract (CIE) for increasing cell growth, alkaline phosphatase (ALP) activity, and collagen content were totally inhibited, suggesting that the effect of CIE might be partly involved with estrogen activity. Furthermore, the protective effects of CIE on the response of osteoblasts to oxidative stress were evaluated. Osteoblastic MC3T3-E1 cells were incubated with hydrogen peroxide and/or CIE, and markers of osteoblast function and oxidative damage were examined. CIE significantly increased cell survival, ALP activity, and calcium deposition, and decreased the production of Reactive Oxygen Species (ROS) and Tumor Necrosis Factor-${\alpha}$ (TNF-${\alpha}$) in osteoblasts. Taken together, these results indicate that the enhancement of osteoblast function by CIE may prevent osteoporosis and inflammatory bone diseases.
Keywords
Chrysanthemum indicum L; osteoblast; MC3T3-E1 cell; oxidative stress; hydrogen peroxide;
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