Browse > Article
http://dx.doi.org/10.9721/KJFST.2012.44.1.076

A Study on the Depigmenting Effect of Carthamus tinctorius Seed, Cyperus rotundus and Schizonepeta tenuifolia Extracts  

Hwang, Eun-Young (Korea Promotion Institute for Oriental Medicine Industry)
Kim, Dong-Hee (Korea Promotion Institute for Oriental Medicine Industry)
Hwang, Jo-Young (Korea Promotion Institute for Oriental Medicine Industry)
Kim, Hui-Jeong (Korea Promotion Institute for Oriental Medicine Industry)
Park, Tae-Soon (Korea Promotion Institute for Oriental Medicine Industry)
Lee, In-Sun (Department of Food and Technology, Keimyung University)
Son, Jun-Ho (Korea Promotion Institute for Oriental Medicine Industry)
Publication Information
Korean Journal of Food Science and Technology / v.44, no.1, 2012 , pp. 76-81 More about this Journal
Abstract
The objective of the present study was to evaluate the skin depigmentation effect of the extracts of three herbs, Carthamus tinctorius seed, Cyperus rotundus and Schizonepeta tenuifolia. Their effects on tyrosinase and melanin synthesis inhibitory action were assessed. We found that the C. tinctorius seed ethanol extracts reduced the tyrosinase activity and melanin formation of B16F10 melanoma cells. The C. tinctorius seed suppressed the expression in microphthalmia associated transcription factor (MITF), tyrosinase, tyrosinase related protein 1 (TRP-1), and tyrosinase related protein 2 (TRP-2) in B16F10 melanoma cells. These results show that C. tinctorius seed inhibited melanogenesis on the B16F10 melanoma cell. The underlying mechanism of C. tinctorius seed whitening activity may be the inhibition of tyrisinase, MITF, tyrosinase, TRP-1, and TRP-2 expression. The results suggested that C. tinctorius seed has considerable potential as a natural functional ingredient with a depigmentation effect.
Keywords
Carthamus tinctorius seed; tyrosinase; B16F10 melanoma cell; melanin synthesis; depigmenting effect;
Citations & Related Records
Times Cited By KSCI : 7  (Citation Analysis)
연도 인용수 순위
1 Oh HC, Lim KS, Hwang CY, Youn IH, Kim NK. A Study on the melanin synthesis inhibition and whitening effect of bombysis corpus. Korean J. Oriental Med. 20: 1-13 (2007)   과학기술학회마을
2 Park SH, Lee BY, Han CS, Kim JG, Kim KT, Kim KH, Kim YH. Whitening effect of Angelica keiskei koidzumi extract by inhibition of endothelin-1 production and melanogenesis. Korean J. Soc. Cosmet. Sci. 34: 101-107 (2008)   과학기술학회마을
3 Kim TH, You JK, Kim JM, Baek JM, Kim HS, Park JH, Choe M. Antioxidant and whitening effects of Sorbus commixta hecl cortex Extract. Korean J. Soc. Food Sci. Nutr. 39: 1418-1424 (2010)   DOI
4 Seung KR, Jung KH. Effect of Carthamus tinctorius L. semen on endotoxin-induced thrombosis in rats. J. Pharmacol. 9: 258-262 (2001)
5 Jung KW, Jeong CS, Jeong JS. Effect of subfractions of Carthamus tinctorius L. semen on the lipid peroxidation and oxygen free radical scavenging enzyme activities in CCI4-induced hepatotoxic rats. J. Food Hyg. Safety 14: 179-185 (1999)
6 Park JY. Comparative studies on the physiological activities of processed and unprocessed cyperi rhizomata. PhD thesis, Sookmyung Women's University, Seoul, Korea (1996)
7 Hikino H. Apta K, Takemoto T. Structure and absolute configuration of Cyperotundone. Chem Pharm. Bull. 14: 890-896 (1966)   DOI
8 Gupta MB, Palit TK, Singh N, Bhargava KP. Pharmacological studies to isolate the active constituents from Cyperus rotundus possing anti-inflammatory, anti-pyretic, and analgesic activities. Indian J. Med. Res 59: 76-82 (1970)
9 Moon JY. Distinction and phylogenetic analysies of Citri unshiu semen, Schizonepetae spike and Prunella spike on basis of DNA sequences. PhD thesis, Kyunghee University, Seoul, Korea (2011)
10 Yagi AT, Kanbara T, Morinobu N. The effect of tyrosinase inhibition for aloe. Planta Med. 3981: 517-519 (1986)
11 Michikawa M, Lim KT, McLarnon JG, Kim SU. Oxygen radicalinduced neurotoxicity in spinal cord neuron cultures. J. Neurosci. Res. 37: 62-70 (1994)   DOI
12 Carmichael J, Defraff WG, Gazdar AF, Minna JD, Mitchell JB. Evaluation of a tetrazolium based semiautomated colorimetric assay: Assessment of chemosensitivuty tesing. Cancer Res. 47: 936-942 (1987)
13 Hosoi JE, Abe T, Suda T, Kuroki. Regulation of melanin synthesis of B16 melanoma cells by 1 alpha, 25-dihydroxyvitamin D3 and retinoic acid. J. Cancer. Res. 45: 1474-1478 (1985)
14 Cho YJ. Study om the tyrosinase activity inhibition and free radical scavenging activity of natural materials. MS thesis, Sookmyung Womans University, Seoul, Korea. (2004)
15 Lee NH, Yang HC, Bu HJ, Jung DS, Lee SJ, Riu KZ. Screening of the tyrosinase inhibition and hyaluronidase inhibition activities and radical scavenging effects using plants in Jeju. Korean J. Pharmacogn. 32:175-180 (2001)
16 Carmichael J, Degraff WG, Gadzar AF. Evaluation of a tetrazolium based semiautomated colorimetric assay: Assessment of chemosensitivity testing. Cancer Res. 477: 936-942 (1987)
17 Yu YG, Jeong MS, Choe JY, Kim JY. A study on whitening effect of Ephedra sinica extracts. Korean J. Design Cult. Soc. 31: 153-159 (2005)
18 Sapkota K, Park SE, Kim JE, Kim S, Choi HS, Chun HS, Kim SJ. Antioxidant and antimelanogenic properties of chestnut flower extract. Biosci. Biotech. Bioch. 74: 1527-1533 (2010)   DOI
19 Ha JH, Kwon MC, Kim SS, Jeong MH, Hwang B, Lee HY. Enhancement of skin-whitening and UV-protective effects of Centella asiatica L. Urban by Utrasonification Pross. Korean J. Medicinal Crop Sci. 18: 79-85 (2010)
20 Han GS, Kim DS, Woo WH, Mun YJ. Inhibitory effect of fructus Ligustri Lucidi on tyrosinase and MITF expressions. Korean J. Orient. Physiol. Pathol. 24: 296-301 (2010)   과학기술학회마을
21 Jeong MH, Kim SS, Kim JS, Lee HJ, Chio GP, Lee HY. Jour. Skin whitening and skin immune activities of different parts of Acer mono and Acer okamotoanum. Korean For. Soc. 99: 470- 478 (2005)
22 Chen JS, Wei C, Marxhall MR. Inhibition mechanism of koji acid on polyphenol oxidase. J. Agr. Food Chem. 58: 79-110 (1991)
23 Urabe K, Aroca P, Tsukamoto K, Mascagna D, Paulumbo A, Prota G, Hering VJ. The inherent cytotoxicty of melanin precursors. Biochim. Biophys. Acta 1221: 272-278 (1994)   DOI
24 Hearing VJ, Tsukamoto K. Enzymatic control of pigmentation in mammals. FASEB J. 5: 2902-2909 (1991)
25 Jimenez-Cervantes C, Solano F, Kobayashi T, Urabe K, hearing V, Lezano J, Garcia-Gorreni JC. A new enzymatic function in the melanogenic pathway. J. Biol. Chem. 269: 17993-18000 (1994)
26 Parvez S, Malik K, Ah KS, Kim HY. Probiotics and their fermented food products are beneficial for health. J. Appl. Microbiol. 100: 1171-1185 (2006)   DOI
27 Maeda K, Fukuda M. Arbutin: Mechanism of its depigmenting action in human melanocyte culture. J. Phamacol. Exp. Ther. 276: 765-769 (1996)
28 Busca R, Ballotti R. Cyclic AMP a key messenger in the regulation of skin pigmentation. Pigm. Cell Res. 13: 60-69 (2000)   DOI
29 Sassone CP. Coupling gene expression to cAMP signalling: Role of CREB and CREM. Int. J. Biochem. Cell B. 30: 27-38 (1998)   DOI
30 Saha B, Singh SK, Sarkar C, Bera R, Ratha J, Tobin DJ, Bhadra R. Activation of the Mitf promoter by lipid-stimulated activation of p38-stress signalling to CREB. Pigm. Cell Res. 29: 595-605 (2006)
31 Battaini GE, Monzani L, Casella L, Santagostini R, Pagliarin. Inhibition of the catecholase activity of biomimetic dinuclear copper complexes by kojic acid. J. Biol. Inorg. Chem. 5: 262-268 (2000)   DOI
32 Chun HJ, Chli WH, Baek SH, Woo WH. Effect of quercetin on melanogenesis in melan-a melanocyte cells. Korean J. Pharmacogn. 33: 245-251 (2002)   과학기술학회마을
33 Curto EV, Kwong C, Hermersdorfer H, Glatt C, Santis V, Virador VJ and Dooley TP. Inhibitions of mammalian melanocytes tyrosinase: In vitro comparisons of alkyl esters of gentiic acid with other putative inhibitors. Biochem. Pharmacol. 57: 663-672 (1999)   DOI
34 Seo BI, Lee ES, Park JH, Kim SC, Byun BH, Choi HY. A efficacy of Korean and Chinese Carthami Semen on analgesic effect, anti-inflammation and arthritis. Korean J. Herbol. 16: 1-10 (2001)   과학기술학회마을
35 Hur TY, Yun MY, Cho EH, Lee OJ, Kim KS, Cho NG. Clinical study on dffect of carthmi-Flos Herbal acupuncture therapy on osteoarthritis in knee joint. Korean J. Acupunct. Moxibustion Soc. 19: 189-200 (2002)