Browse > Article
http://dx.doi.org/10.9721/KJFST.2011.43.6.689

Optimization of Lipase-Catalyzed Interesterification for Production of Human Milk Fat Substitutes by Response Surface Methodology  

Son, Jeoung-Mae (Department of Food Science and Technology, Chungnam National University)
Lee, Jeung-Hee (Department of Food Science and Technology, Chungnam National University)
Xue, Cheng-Lian (Department of Food Science and Technology, Chungnam National University)
Hong, Soon-Taek (Department of Food Science and Technology, Chungnam National University)
Lee, Ki-Teak (Department of Food Science and Technology, Chungnam National University)
Publication Information
Korean Journal of Food Science and Technology / v.43, no.6, 2011 , pp. 689-695 More about this Journal
Abstract
1,3-Dioleoyl-2-palmitoylglycerol (OPO)-rich human milk fat substitute (HMFS) was synthesized from tripalmitin (PPP)-rich fraction and oleic ethyl ester by a lipase-catalyzed interesterification. Response surface methodology (RSM) was employed to optimize the presence of palmitic acid at sn-2 position ($Y_1$, %) and of oleic acid at sn-1,3 ($Y_2$, %), with the reaction factors as substrate molar ratio of PPP-rich fraction to oleic ethyl ester ($X_1$, 1:4, 1:5 and 1:6), reaction temperature ($X_2$, 50, 55 and $60^{\circ}C$), and time ($X_3$, 3, 7.5 and 12 h). The optimal conditions for HMFS synthesis were predicted at the reaction combination of $55^{\circ}C$, 3 h and 1:6 substrate ratio. HMFS re-synthesized under the same conditions displayed 70.70% palmitic acid at the sn-2 position and 69.58% oleic acid at the sn-1,3 position. Reaction product was predominantly (90.35%) triacylglycerol (TAG) was observed in which the major TAG species, OPO, comprised 31.24%.
Keywords
human milk fat substrate (HMFS); tripalmitin; oleic ethyl ester; response surface methodology;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Park CW, Bahn KN, Lee YN, Kim JO, Kim MS, Ha YL. Conjugated linoleic acid in Korean mothers' milk and infant formula. J. Korean Soc. Food Sci. Nutr. 36: 371-375 (2007)
2 AOCS. Official Methods and recommended practices of the AOCS. 4thed. Method Ca 5a-40, Ce 2-66. American Oil Chemists' Society Press, Champaign, IL, USA (1990)
3 Carroll KK. Separation of lipid classes by chromatography on florisil. J. Lipid Res. 2: 135-141 (1961)
4 Alim, MA, Lee JH, Shin JA, Lee YJ, Choi MS, Akoh CC, Lee KT. Lipase-catalyzed production of solid fat stock from fractionated rice bran oil, palm stearin, and conjugated linoleic acid by response surface methodology. Food Chem. 106: 712-719 (2008)   DOI   ScienceOn
5 Lee JH, Kim MR, Kim IH, Kim H, Shin JA, Lee KT. Physicochemical and volatile characterization of structured lipids from olive oil produced in a stirred-tank batch reactor. J. Food Sci. 69: 89-95 (2004)
6 Harfmann RG, Julka S, Cortes HJ. Instability of hexane-acetonitrile mobile phases used for the chromatographic analysis of triacylglycerides. J. Sep. Sci. 31: 915-920 (2008)   DOI   ScienceOn
7 Srivastava A, Akoh CC, Chang SW, Lee GC, Shaw JF. Candida rugosa lipase LIP1-catalyzed transesterification to produce human milk fat substitute. J. Agr. Food Chem. 54: 5175-5181 (2006)   DOI   ScienceOn
8 Neff WE, Adlof RO, List GR, EL-Agaimy M. Analysis of vegetable oil triacylglycerols by silver ion high performance liquid chromatography with flame ionization detection. J. Liquid Chromatogr. 17: 3951-3968 (1994)   DOI   ScienceOn
9 Macrae AR. Lipase-catalyzed interesterification of oils and fats. J. Am. Oil Chem. Soc. 60: 291-294 (1983)   DOI   ScienceOn
10 Jin HS. Development of Korean dairy industry-infant formula. J. Korean Dairy Technol. Sci. 23: 115-160 (2005)
11 Chen ML, Vali SR, Lin JY, Ju YH. Synthesis of the structured lipid 1,3-dioleoyl-2-palmitoyl glycerol from palm oil. J. Am. Oil Chem. Soc. 81: 525-532 (2004)   DOI   ScienceOn
12 Aoe S, Yamamura J, Matsuyama H, Hase M, Shiota M, Miura S. The positional distribution of dioleoyl-palmitoyl glycerol influences lymph chylomicron transport, composition, and size rats. J. Nutr. 1269-1273 (1997)
13 Akoh CC. pp. 261-277. In: Clinical benefits of a structured lipid ($Betapol^{TM}$) in infant formula. Handbook of Functional Lipid. Taylor & Francis A CRC Press, USA (2006)
14 Carnielli VP, Luijendijk, IHT, Van Goudoever JB, Sulkers EJ, Boerlage AA, Degenhart HJ, Sauer PJJ. Structure position and amount of palmitic acid in infant formula: Effects on fat, fatty acid, and mineral balance. J. Pediatr. Gastr. Nutr. 23: 553-560 (1996)   DOI   ScienceOn
15 Kennedy K, Fewtrell MS, Morley R, Abbott R, Quinlan PT, Wells JCK, Bindels JG, Lucas A. Double-blind, randomized trial of a synthetic triacylglycerol in formula-fed term infants: Effects on stool biochemistry, stool characteristics, and bone mineralization. Am. J. Clin. Nutr. 70: 920-927 (1999)
16 Nelsom CM, Innis SM. Plasma lipoprotein fatty acids are altered by the positional distribution of fatty acids in infant formula triacylglycerols and human milk. Am. J. Clin. Nutr. 70: 62-69 (1999)
17 Lee KT, Akoh CC. Structured lipids: Synthesis and applications. Food Rev. Int. 14:17-34 (1998)   DOI   ScienceOn
18 Akoh CC. Structured lipid: Enzymatic approach. Inform 6: 1055-1061 (1995)
19 Sahin N, Akoh CC, Karaali A. lipase-catalyzed acidolysis of tripalmitin with hazelnut oil fatty acids and stearic acid to produce human milk fat substitutes. J. Agr. Food Chem. 53: 5779-5783 (2005)   DOI   ScienceOn
20 Yang T, Xu X, He C, Li L. lipase-catalyzed modification of lard to produce human milk fat substitutes. Food Chem. 80: 473-481 (2003)   DOI   ScienceOn
21 Maduko CO, Akoh CC, Park YW. Enzymatic interesterification of tripalmitin with vegetable oil blends for formulation of caprine milk infant formula analogs. J. Dairy Sci. 90: 594-601 (2007)   DOI   ScienceOn