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http://dx.doi.org/10.9721/KJFST.2011.43.1.072

Mucosal Immunoadjuvant Activity of Korean Mistletoe Lectin-C  

Kim, Jin-Chul (Institute for Biomedical Research, Handong University)
Yoon, Taek-Joon (Department of Food & Nutrition, Yuhan University)
Song, Tae-Jun (Institute for Biomedical Research, Handong University)
Kim, Young-Hoon (Institute for Biomedical Research, Handong University)
An, Hyo-Sun (Institute for Biomedical Research, Handong University)
Kim, Jong-Bae (Institute for Biomedical Research, Handong University)
Publication Information
Korean Journal of Food Science and Technology / v.43, no.1, 2011 , pp. 72-76 More about this Journal
Abstract
The adjuvant effects of Korean mistletoe lectin-C (KML-C) were investigated following the oral administration of KML-C with ovalbumin (OVA) as an antigen. Mice were orally immunized with OVA alone or admixed with various doses of KML-C or cholera toxin (CT), and the titer of OVA-specific antibody in the serum and mucosal secretions were determined. OVA+KML-C-treated mice showed high titers of IgA specific to CT in mucosal secretions. The antibody titers in the serum of OVA+KML-C-treated mice were comparable to those in the serum of OVA+CT-treated mice. When mice were immunized with OVA+KML-C or with CT alone and subsequently injected with OVA on the footpads after the primary immunization, they showed a more significant increase in delayed-type hypersensitivity reactions than when they were administered CT alone. These results suggest that KML-C is a potent immunoadjuvant that enhances both humoral and cellular immunity by the mucosal immune system.
Keywords
mistletoe lectin-c; mucosal immune response; immunoadjuvant; oral administration; IgA;
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1 Pelletier RP, Bickerstaff AA, Adams PW, Orosz CG. Evaluation of immune regulation in transplant patients using the trans vivo delayed type hypersensitivity assay. Hum. Immunol. 68: 514-522 (2007)   DOI   ScienceOn
2 Kim HW, Chew BP, Wong TS, Park JS, Weng BB, Byrne KM, Hayek MG, Reinhart GA. Modulation of humoral and cell-mediated immune responses by dietary lutein in cats. Vet. Immunol. Immunop. 73: 331-341 (2000)   DOI   ScienceOn
3 Phumiamorn S, Sato H, Kamiyama T, Kurokawa M, Shiraki K. Induction of humoral and cell-mediated immunity to hepatitis B surface antigen by a novel adjuvant activity of Oka varicella vaccine. J. Gen. Virol. 84: 287-291 (2003)   DOI   ScienceOn
4 Lyu SY, Park WB. Mistletoe lectin modulates intestinal epithelial cell-derived cytokines and B cell IgA secretion. Arch. Pharm. Res. 32: 443-451 (2009)   DOI
5 Yoo YC, Yoshimatsu K, Koike Y, Hatsuse R, Yamanishi K, Tanishita O, Arikawa J, Azuma I. Adjuvant activity of muramyl dipeptide derivatives to enhance immunogenicity of a hantavirusinactivated vaccine. Vaccine 16: 216-224 (1998)   DOI   ScienceOn
6 Sjoblom-Hallen A, Marklund U, Nerstedt A, Schon K, Ekman L, Bergqvist P, Lowenadler B, Lycke NY. Gene expression profiling identifies STAT3 as a novel pathway for immunomodulation by cholera toxin adjuvant. Mucosal Immunol. 3: 374-386 (2010)   DOI   ScienceOn
7 Eriksson K, Fredriksson M, Nordstrom I, Holmgren J. Cholera toxin and its B subunit promote dendritic cell vaccination with different influences on Th1 and Th2 development. Infect. Immun. 71: 1740-1747 (2003)   DOI   ScienceOn
8 Su SB, Silver PB, Wang P, Chan CC, Caspi RR. Cholera toxin prevents Th1-mediated autoimmune disease by inducing immune deviation. J. Immunol. 173: 755-761 (2004)   DOI
9 Boneberg EM, Hartung T. Mistletoe lectin-1 increases tumor necrosis factor-alpha release in lipopolysaccharide-stimulated whole blood via inhibition of interleukin-10 production. J. Pharmacol. Exp. Ther. 298: 996-1000 (2001)
10 Li L, Elliott JF, Mosmann TR. IL-10 inhibits cytokine production, vascular leakage, and swelling during T helper 1 cell-induced delayed-type hypersensitivity. J. Immunol. 153: 3967-3978 (1994)
11 Lyu SY, Park WB. Effects of Korean mistletoe lectin (Viscum album coloratum) on proliferation and cytokine expression in human peripheral blood mononuclear cells and T-lymphocytes. Arch. Pharm. Res. 30: 1252-1264 (2007)   DOI
12 Yoon TJ, Yoo YC, Kang TB, Baek YJ, Huh CS, Song SK, Lee KH, Azuma I, Kim JB. Prophylactic effect of Korean mistletoe (Viscum album coloratum) extract on tumor metastasis is mediated by enhancement of NK cell activity. Int J. Immunopharmaco. 20: 163-172 (1998)   DOI   ScienceOn
13 Yoon TJ, Yoo YC, Kang TB, Song SK, Lee KB, Her E, Song KS, Kim JB. Antitumor activity of the Korean mistletoe lectin is attributed to activation of macrophages and NK cells. Arch. Pharm. Res. 26: 861-867 (2003)   DOI
14 Hajto T, Hostanska K, Frei K, Rordorf C, Gabius HJ. Increased secretion of tumor necrosis factors alpha, interleukin 1, and interleukin 6 by human mononuclear cells exposed to beta-galactoside- specific lectin from clinically applied mistletoe extract. Cancer Res. 50: 3322-3326 (1990)
15 Lyu SY, Park WB. Transport of mistletoe lectin by M cells in human intestinal follicle-associated epithelium (FAE) in vitro. Arch. Pharm. Res. 31: 1613-1621 (2008)   DOI
16 Mueller EA, Anderer FA. Chemical specificity of effector cell/ tumor cell bridging by a Viscum album rhamnogalacturonan enhancing cytotoxicity of human NK cells. Immunopharmacology 19: 69-77 (1990)   DOI   ScienceOn
17 Mueller EA, Anderer FA. A Viscum album oligosaccharide activating human natural cytotoxicity is an interferon gamma inducer. Cancer Immunol. Immun. 32: 221-227 (1990)   DOI   ScienceOn
18 Kang TB, Song SK, Yoon TJ, Yoo YC, Lee KH, Her E, Kim JB. Isolation and characterization of two Korean mistletoe lectins. J. Biochem. Mol. Biol. 40: 959-965 (2007)   DOI
19 Metzner G, Franz H, Kindt A, Fahlbusch B, Suss J. The in vitro activity of lectin I from mistletoe (ML I) and its isolated A and B chains on functions of macrophages and polymorphonuclear cells. Immunobiology 169: 461-471 (1985)   DOI
20 Debray H, Montreuil J, Franz H. Fine sugar specificity of the mistletoe (Viscum album) lectin I. Glycoconjugate J. 11: 550-557 (1994)   DOI   ScienceOn
21 Kuhara T, Iigo M, Itoh T, Ushida Y, Sekine K, Terada N, Okamura H, Tsuda H. Orally administered lactoferrin exerts an antimetastatic effect and enhances production of IL-18 in the intestinal epithelium. Nutr. Cancer 38: 192-199 (2000)   DOI   ScienceOn
22 Ghose C, Kalsy A, Sheikh A, Rollenhagen J, John M, Young J, Rollins SM, Qadri F, Calderwood SB, Kelly CP, Ryan ET. Transcutaneous immunization with Clostridium difficile toxoid A induces systemic and mucosal immune responses and toxin Aneutralizing antibodies in mice. Infect. Immun. 75: 2826-2832 (2007)   DOI   ScienceOn
23 Lavelle EC, Grant G, Pusztai A, Pfuller U, O'Hagan DT. The identification of plant lectins with mucosal adjuvant activity. Immunology 102: 77-86 (2001)   DOI   ScienceOn
24 Holmgren J, Adamsson J, Anjuere F, Clemens J, Czerkinsky C, Eriksson K, Flach CF, George-Chandy A, Harandi AM, Lebens M, Lehner T, Lindblad M, Nygren E, Raghavan S, Sanchez J, Stanford M, Sun JB, Svennerholm AM, Tengvall S. Mucosal adjuvants and anti-infection and anti-immunopathology vaccines based on cholera toxin, cholera toxin B subunit and CpG DNA. Immunol. Lett. 97: 181-188 (2005)   DOI   ScienceOn
25 Stern ME, Schaumburg CS, Dana R, Calonge M, Niederkorn JY, Pflugfelder SC. Autoimmunity at the ocular surface: Pathogenesis and regulation. Mucosal Immunol. 3: 425-442 (2010)   DOI   ScienceOn
26 Anosova NG, Chabot S, Shreedhar V, Borawski JA, Dickinson BL, Neutra MR. Cholera toxin, E. coli heat-labile toxin, and nontoxic derivatives induce dendritic cell migration into the follicleassociated epithelium of Peyer's patches. Mucosal Immunol. 1: 59-67 (2008)   DOI   ScienceOn
27 Elmore SA. Enhanced histopathology of mucosa-associated lymphoid tissue. Toxicol. Pathol. 34: 687-696 (2006)   DOI   ScienceOn
28 Gong Z, Jin Y, Zhang Y. Oral administration of a cholera toxin B subunit-insulin fusion protein produced in silkworm protects against autoimmune diabetes. J. Biotechnol. 119: 93-105 (2005)   DOI   ScienceOn
29 Kelsall BL. A focus on dendritic cells and macrophages as key regulators of mucosal immunity. Mucosal Immunol. 1: 423-424 (2008)   DOI   ScienceOn
30 Flo J, Elias F, Benedetti R, Massouh E. Reversible effects on B and T cells of the gut-associated lymphoid tissues in rats malnourished during suckling: Impaired induction of the immune response to intra-Peyer patches immunization with cholera toxin. Clin. Immunol. Immuno. 80: 147-154 (1996)   DOI   ScienceOn
31 Hibi M, Hachimura S, Ise W, Sato A, Yoshida T, Takayama T, Sasaki K, Senga T, Hashizume S, Totsuka M, Kaminogawa S. Dendritic cells from spleen, mesenteric lymph node, and Peyer's patch can induce the production of both IL-4 and IFN-gamma from primary cultures of naive CD4(+) T cells in a dose-dependent manner. Cytotechnology 43: 49-55 (2003)   DOI
32 Lavelle EC, Grant G, Pusztai A, Pfuller U, O'Hagan DT. Mucosal immunogenicity of plant lectins in mice. Immunology 99: 30-37 (2000)   DOI   ScienceOn