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http://dx.doi.org/10.9721/KJFST.2011.43.1.001

Analysis and Monitoring of Residues of Aminoglycoside Antibiotics in Livestock Products  

Kang, Young-Woon (Food Contaminants Division, Food Safety Evaluation Department, NIFDS)
Joo, Hyun-Jin (Hazardous Substances Analysis Division, Gyeongin Regional KFDA)
Kim, Yang-Sun (Imported food Analysis Division, Gyeongin Regional KFDA)
Cho, Yu-Jin (Imported food Analysis Division, Gyeongin Regional KFDA)
Kim, Hee-Yun (Hazardous Substances Analysis Division, Seoul Regional KFDA)
Lee, Gwang-Ho (Food Contaminants Division, Food Safety Evaluation Department, NIFDS)
Kim, Mee-Hye (Food Contaminants Division, Food Safety Evaluation Department, NIFDS)
Publication Information
Korean Journal of Food Science and Technology / v.43, no.1, 2011 , pp. 1-5 More about this Journal
Abstract
It is possible that veterinary medicines remain in livestock food products, according to the use of many and various veterinary medicines to protect against disease when livestock animals are breed in limited space. Concentrated and continuous monitoring of residues is needed due to increases in resistance to antibiotics and side effects by eating livestock food products. We developed an analysis method for detecting streptomycin, dihydrostreptomycin, neomycin, gentamicin and spectinomycin in meat using LC/MS/MS and measured sensitivity, precision, accuracy, linearity and recovery according to CODEX guidelines to acquire confidence in the analysis method. Based on the results, we acquired good sensitivity compared to the maximum residue limit (MRL) as limits of detection (LOD) were 0.002-0.016 mg/kg and limits of quantification (LOQ) were 0.006-0.050 mg/kg. The analysis method satisfied the CODEX guidelines. The linearity ($r^2$) values of aminoglycoside antibiotics were 0.9936-0.9980, recoveries were 60-110% and relative standard deviations (RSD) were within 15%. As a result of monitoring for residues in a total 250 samples of livestock foods such as pork, chicken, and beef by the confirmed method, dihydrostreptomycin and gentamicin were detected in 5 pork samples. The residues of these antibiotics were within the MRLs. Thus, the detection ratio was 2% as 5 samples were identified from 250 samples.
Keywords
livestock; antibiotics; residues; aminoglycoside; mass spectrometer;
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