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Inhibitory Effects of Punica granatum L. Extracts on Degranulation in Human Basophilic KU812F Cells  

Park, Kyong-Tae (Department of Culinary Arts & Nutrition, Kaya University)
Shim, Sun-Yup (Institute of Marine Life Science, Pukyong National University)
Chun, Soon-Sil (Department of Food and Nutrition, Sunchon National University)
Publication Information
Korean Journal of Food Science and Technology / v.40, no.6, 2008 , pp. 702-706 More about this Journal
Abstract
Punica granatum (PG) evidences a variety of physiological properties, including anti-diabetic, anti-cancer, antiinflammatory, anti-microbial, and anti-oxidative activities. Using the human basophilic KU812F cells, the inhibitory effects of the methanolic extract of PG seed, shell, and juice on calcium ionophore, A23187-induced degranulation were assessed. All of the PG extracts inhibited A23187-induced intracellular $Ca^{2+}$ levels, ${\beta}$-hexosaminidase, and histamine release in a dose-dependent manner. These results showed that all of the PG extracts are potent inhibitors of degranulation in allergic reactions, via the suppression of $Ca^{2+}$ influx.
Keywords
Punica granatum L.; calcium influx; histamine; $\beta$-hexosaminidase; KU812F;
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