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Skin Whitening and Skin Immune Activities of the Peptides Isolated from Crassostrea gigas by Ultrasonification Processes  

Han, Jae-Gun (Bio Materials Engineering, College of Bioscience and Biotechnology, Kangwon National University)
Kim, Hyo-Sung (Bio Materials Engineering, College of Bioscience and Biotechnology, Kangwon National University)
Kwon, Min-Chul (Bio Materials Engineering, College of Bioscience and Biotechnology, Kangwon National University)
Kim, Jin-Chul (Bio Materials Engineering, College of Bioscience and Biotechnology, Kangwon National University)
Hwang, Bo-Young (SKY007 Co.)
Lee, Hyeon-Yong (College of Bioscience and Biotechnology, Kangwon National University)
Publication Information
Korean Journal of Food Science and Technology / v.40, no.4, 2008 , pp. 394-399 More about this Journal
Abstract
In this study, peptides were isolated from Crassostrea gigas using an ultrasonification process at $40^{\circ}C$. The yield of the peptides was greater than 34%, and their cytotoxicity was found to be less than 22.8% against several cell lines that were treated with the extracts at a dose of 1.0 mg/mL. In addition, the tyrosinase inhibitory and melanin synthesis of the peptides isolated from Crassostrea gigas were also evaluated to determine if they could be used as a potential cosmetic agent. The peptides were found to significantly inhibit the melanin synthesis of the clone M-3 cell line by up to 62.7%. The inhibitory activities of the tyrosinase were observed 34.51% in ascorbic acid, 42.49% in extract with the ultrasonification at $40^{\circ}C$ and 35.37% in $40^{\circ}C$ extract at 1.0 mg/mL concentration, respectively. Finally, when samples were treated with the peptide extracts at a concentration of 0.6 mg/mL, PGE2 expression was significantly decreased. Taken together, these results indicate that Crassostrea gigas may be a source of cosmetic agents capable of improving physiological hyperpigmenting and immuno-modulating skin disorders.
Keywords
Crassostrea gigas; skin immuno-modulating; skin whitening effect; melanin; ultrasonification process;
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