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In vitro Anti-diabetic Effects of Crude Extracts of Platycodi Radix  

Ko, Byoung-Seob (Herbal Quality Control Team, Korea Institute of Oriental Medicine)
Kwon, Dae-Young (Food Functional Research Division, Korea Food Research Institutes)
Hong, Sang-Mee (Department of Food and Nutrition)
Park, Sun-Min (Institute of Basic Sciences, Hoseo University)
Publication Information
Korean Journal of Food Science and Technology / v.39, no.6, 2007 , pp. 701-707 More about this Journal
Abstract
Anti-diabetic effect of Platycodi radix (PR) extract fractions was determined if vitro by investigating insulin-like action, insulin sensitizing action, glucose-stimulated insulin secretion, gene expression related to ${\beta}-cell$ function and mass, and ${\alpha}$-glucoamylase suppressing action. Insulin-like activity was not promoted by the treatment of PR methanol factions in 373-L1 fibroblast. However, treatment with 0, 20 and 100% PR methanol fractions along with 1 ng/mL insulin increased insulin-stimulated glucose uptake in 373-L1 adipocytes. In addition, the treatment of 0% and 100% methanol fractions along with differentiation inducers significantly increased the differentiation of 373-L1 fibroblasts to adipocytes. These fractions may contain insulin sensitizer. The 20%, 80% and 100% methanol fractions enhanced glucose-stimulated insulin secretion in Min6 cells, insulin secreting cell line. This was related to the mechanism to promote glucose sensing and ${\beta}-cell$ proliferation, which was regulated by the induction of IRS-2, glucokinase and PDX-1 genes. As expected, 20, 80 and 100% methanol fractions increased mRNA levels of IRS-2, glucokinase and PDX-1 genes. However, PR fractions did not affect the ${\alpha}-glucoamylase$ activity in vitro. These data suggested that PR extract fractions have anti-diabetic actions through improving insulin sensitization, glucose-stimulated insulin secretion, and ${\beta}-cell$ proliferation. Therefore, PR extracts can be beneficial for anti-diabetic treatment in lean diabetic patients.
Keywords
373-L1 adipocyte; insulin sensitizer; IRS2; PDX-1; glucokinase;
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Times Cited By KSCI : 2  (Citation Analysis)
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