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In vitro Anti-inflammatory Activity of the Artemisia fukudo Extracts in Murine Macrophage RAW 264.7 Cells  

Yoon, Weon-Jong (Jeju Biodiversity Research Institute, Jeju Hi-Tech Industry Development Institute)
Lee, Jung-A (Jeju Biodiversity Research Institute, Jeju Hi-Tech Industry Development Institute)
Kim, Kil-Nam (Jeju Biodiversity Research Institute, Jeju Hi-Tech Industry Development Institute)
Kim, Ji-Young (Jeju Biodiversity Research Institute, Jeju Hi-Tech Industry Development Institute)
Park, Soo-Yeong (Jeju Biodiversity Research Institute, Jeju Hi-Tech Industry Development Institute)
Publication Information
Korean Journal of Food Science and Technology / v.39, no.4, 2007 , pp. 464-469 More about this Journal
Abstract
The present study describes the preliminary evaluation of the anti-inflammatory activities of Artemisia fukudo extracts. The 80% ethanol extract of A. fukudo was sequentially fractionated with n-hexane, dichloromethane, ethylacetate, and butanol. In order to effectively screen for anti-inflammatory agents, we first examined the extracts’ inhibitory effects on the production of pro-inflammatory cytokines activated with lipopolysaccharide. Moreover, we examined the inhibitory effects of the A. fukudo extracts on pro-inflammatory factors (NO, iNOS, COX-2, and $PGE_{2}$) in murine macrophage RAW 264.7 cells. The protein levels were determined by immunoblotting. Of the sequential solvent fractions, the n-hexane and dichloromethane fractions inhibited the mRNA expression of pro-inflammatory cytokines (TNF-${\alpha}$, IL-$1{\beta}$, and IL-6), production of NO and $PGE_{2}$, and the protein levels of iNOS and COX-2. These results suggest that A. fukudo may have signifIcant effects on inflammatory factors, and may be a potential anti-inflammatory therapeutic plant.
Keywords
Artemisia fukudo; pro-inflammatory cytokines; NO; $PGE_{2}$; iNOS; COX-2;
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