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Anti-diabetic Effect of Crude Polysaccharides from Grifola frondosa in $KK-A^{y}$ Diabetic Mouse and 3T3-L1 Adipocyte  

Park, Kum-Ju (Institute of Food & Culture R&D Center for Functional Food, Pulmuone Co., Ltd.)
Oh, Young-Joo (Institute of Food & Culture R&D Center for Functional Food, Pulmuone Co., Ltd.)
Lee, Sang-Yun (Institute of Food & Culture R&D Center for Functional Food, Pulmuone Co., Ltd.)
Kim, Hyun-Su (Division of Life Sciences, Kyungnam University)
Ha, Hyo-Cheol (Institute of Food & Culture R&D Center for Functional Food, Pulmuone Co., Ltd.)
Publication Information
Korean Journal of Food Science and Technology / v.39, no.3, 2007 , pp. 330-335 More about this Journal
Abstract
This study was performed to investigate the anti-diabetic mechanism of crude polysaccharides isolated from the fruiting bodies of Grifola frondosa. We treated 3T3-L1 adipocyte cells to observe whether the crude polysaccharides isolated from Grifola frondosa would stimulate insulin sensitivity. Significant insulin sensitizing activity was observed in the 3T3-L1 adipocytes, and giving the crude polysaccharide of Grifola frondosa with 1 nM of insulin caused glucose uptake to increase to a similar level as giving 50 nM of insulin alone. To confirm the mechanism for the anti-diabetic effect of the crude polysaccharides, we performed further examinations within $KK-A^{y}$ mice, an animal model of type 2 diabetes. The crude polysaccharides reduced blood glucose levels in the $KK-A^{y}$ mice for 2 weeks after feeding, and also significantly lowered plasma insulin levels. These results suggest that the anti-diabetic mechanism of the crude polysaccharide of Grifola frondosa is related to the enhancement of insulin sensitivity.
Keywords
Grifola frondosa; Crude polysaccharide; 3T3-L1 adipocyte; Insulin sensitivity; $KK-A^{y}$ mice;
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Times Cited By KSCI : 3  (Citation Analysis)
Times Cited By SCOPUS : 2
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