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Induction of Quinone Reductase Activity in Hepatoma Cells by Paprika (Capsicum annuum L.)  

Yu, Mi-Hee (Department of Food Science and Technology, Keimyung University,The Center for Traditional Microorganism Resources, Keimyung University)
Lee, Hyo-Jung (Department of Food Science and Technology, Keimyung University,The Center for Traditional Microorganism Resources, Keimyung University)
Im, Hyo-Gwon (Department of Food Science and Technology, Keimyung University,The Center for Traditional Microorganism Resources, Keimyung University)
Lee, Syng-Ook (Department of Food Science and Technology, Keimyung University,The Center for Traditional Microorganism Resources, Keimyung University)
Lee, In-Seon (Department of Food Science and Technology, Keimyung University,The Center for Traditional Microorganism Resources, Keimyung University)
Publication Information
Korean Journal of Food Science and Technology / v.38, no.5, 2006 , pp. 707-711 More about this Journal
Abstract
Phase 2 enzymes are transcriptionally induced by a wide variety of chemical agents and natural products, and their induction plays a critical role in protection against chemical carcinogens and other toxic xenobiotics. The activity of the methanol extract and fractions of paprika (Capsicum annuum L.) was examined in murine Hepa1c1c7 cells for the induction of nicotinamide adenine dinucleotide (phosphate) NAD(P)H/quinone reductase (QR). The ethyl acetate (EtOAc) fraction induced QR activity in a dose-dependent manner in the concentration range of 10 to $500\;{\mu}g/mL$ with a maximum of a 3.3-fold increase in induction. The EtOAc fraction also showed high QR induction potency in Ah-receptor-defective mutant of Hepa 1c1c7 cells ($BP^rcl$ cells), which indicates that this fraction is a monofunctional inducer of QR. These results suggest that useful cancer chemopreventive materials could be isolated from EtOAc fraction of Paprika.
Keywords
paprika (Capsicum annuum L.); chemoprevention; quinone reductase; monofunctional inducer; Hepa1c1c7;
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Times Cited By KSCI : 2  (Citation Analysis)
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1 Kang Gl. The conception by drugs of medical action and activity. Hiseongchulpansa, Seoul, Korea. pp. 245, 286-302 (1993)
2 Miranda CL, Aponso GLM, Stevens JF, Deinzer ML, Buhler DR. PrenyIated chalcones and flavanones as inducers of quinone reductase in mouse Hepalc1c7 cells. Cancer Lett. 149: 21-29 (2000)   DOI   ScienceOn
3 Zhang Y, Talalay P, Cho CG, Posner GH. A major inducer of anticarcinogenic protective enzymes from broccoli: Isolation and elucidation of structure. Proc. Natl. Acad. Sci. USA. 89: 2399-2403 (1992)
4 Biacs PA, Daood HG, Huszka TT, Biacs PK. Carotenoids and carotenoid esters from new cross cultivars of paprika. J. Agric. Food Chem. 41: 1864-1867 (1993)   DOI   ScienceOn
5 Krinsky Nl. The biological properties of carotenoids. Pure Appl. Chem. 66: 1003-1010 (1994)   DOI   ScienceOn
6 Matsufuji H, Nakamura H, Chino M, Takeda M. Antioxidant activity of capsantin and the fatty acid esters in paprika (Capsicum annuum). J. Agric. Food Chem. 46: 3468-3472 (1998)   DOI   ScienceOn
7 Benson A, Hunkeler MJ, Talay P. Increase of NAD(P)H:qunone reductase by dietary antioxidants. Possible role in protection against carcinogenesis and toxicity. Proc. Natl. Acad. Sci. 77: 5216-5220 (1980)
8 Drysdale BE, Zacharchuk CM, Okajima M, Shin HS. Assay of a cytocidal protein excreted by activated marcophages. pp. 549-555. In: Methods in Enaymology. Sabato D, Eberse T (eds). Academic Press, Orlando, FL, USA (1986)
9 Gerhauser C, You M, Liu J, Moriarty RM, Hawthorne M, Mehta RG, Moon RC, Pezzuto JM. Cancer chemopreventive potential of sulforamate, a novel analogue of sulforaphane that induces phase 2 drug-metabolizing enzymes. Cancer Res. 57: 272-278 (1997)
10 Jeong CH, Ko WH, Cho JR, Ahn CG, Shim KH. Chemical components of Korean paprika according to cultivars. Korean J. Food Preserv. 13: 43-49 (2006)   과학기술학회마을
11 Talalay P, Benson AM. Elvation of quinone reductase activity by anticarcinogenic antioxidants. Adv. Enzyme Regul. 20: 287-300 (1982)   DOI   ScienceOn
12 Prochaska HJ, Santamaria AB. Direct measurement of NAD(P)H: quinone reductase from cells cultured in microtiter well: A screening assay for anticaecinogenic enzyme inducers. Anal. Biochem. 169: 328-336 (1988)   DOI   ScienceOn
13 Choi YM, Kim MH, Shin JJ, Park JM. Lee JS. The antioxidant activities of the some commercial teas. J. Korean Soc. Food Sci. Nutr. 32: 723-727 (2003)   과학기술학회마을   DOI
14 Palevitech D, Craker LE. Nutritional and medicinal importance of red pepper (Capsicum spp.). J. Herbs Spices Med. Plants 3: 5583 (1995)
15 Blois MS. Antioxidant determinations by the use of a stable free radical. J. Agric. Food Chem. 25: 103-107 (1977)   DOI
16 Miliauskas G, Venskutonis PR, Van Beek TA. Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food Chem. 85: 231-237 (2004)   DOI   ScienceOn
17 Mehta RG, Liu J, Constantinou A, Thomas CF, Hawthorne M, Gerhuser C, Pezzuto JM, Moon RC, Moriarty RM. Cancer chemopreventive activity of brassinin, a phytoalexin from cabbage. Carcinogenesis 16: 399-404 (1995)   DOI   ScienceOn
18 Kim SM, Ryu SH, Choi HD, Kim SS, Kim JH, Kim JS. Screening for Korean vegetables with anti carcinogenic enzyme inducing activity using cell culture system. Korean J. Food Sci. Nutr. 27: 277-281 (1998)
19 Spencer SR, Wilczak CA, Talalay P. Induction of glutathione transferases and NAD(P)H: Quinone reductase by fumaric acid derivatives in rodent cells and tissues. Cancer Res. 50: 7871 (1990)
20 Folin O, Denis W. On phosphotungstic-phosopho-molybdic compounds as color reagents. J. Biol. Chem. 12: 239-249 (1912)
21 Talalay P, De Long MJ, Prochaska HJ. Identification of a common chemical signal regulating the induction of enaymes that protect against chemical carcinogenesis. Proc. Natl. Acad. Sci. 85: 8261 (1988)
22 Kim HJ, Jun BS, Kim SK, Cha JY, Cho YS. Polyphenolic compound content and antioxidative activities by extracts from seed, sprout and flower of safflower (Carthamus tinctorius L.). J. Korean Soc. Food Sci. Nutr. 29: 1127-1132 (2000)   과학기술학회마을
23 Wefers H, Komai T, Talalay P, Sies H. Protection against reactive oxygen species by NAD(P)H: quinone recuctase induced by the dietary antioxidant butylated hydroxyanisole (BHA). FEBS Lett. 169: 63-66 (1984)   DOI   ScienceOn
24 Ittah Y, Kanner J, Granity R. Hydrolysis study of carotenoid pigments paprika by HPLC/photodiode array detection. J. Agric. Food Chem. 41: 899-901 (1993)   DOI   ScienceOn
25 Fisher C, Kocis JA. Separation of paprika pigments by HPLC. J. Agric. Food Chem. 35: 55-57 (1987)   DOI
26 Daood HG, Vinkler M, Markus F, Hebshi EA, Biaes PA. Antioxidant vitamin content of spice red pepper (paprika) as affected by technological and variental factors. Food Chem. 55: 365-372 (1996)   DOI   ScienceOn
27 Wachtel RE. Capsaicin. Regist. Anest. Pain Med. 24: 361-363 (1999)   DOI
28 Moreno MIN, Sampietro AR, Vattuone MA. Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. J. Ethnopharmacol. 71: 109-114 (2000)   DOI   ScienceOn
29 Krinsky Nl. Carotenoids as antioxidants. Nutrition 17: 815-817 (2001)   DOI   ScienceOn
30 Maoka T, Mochida K, Kozuka M, Ito Y, Fujiwara Y, Hashimoto K, Enjo F, Ogata M, Nobukuni H, Nishino H. Cancer chemopreventivc activity of carotenoids in the fruits of red paprika Capsicum annuum L. Cancer Lett. 172: 103-109 (2001)   DOI   ScienceOn