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Characteriatics of Single Cell Suspension of Garlic, Red Pepper and Cucumber Prepared by Protopectin Hydrolytic Enzymes  

Baek, Ku-Hyoun (Korea Food Research Institute)
Kim, Sung-Soo (Korea Food Research Institute)
Lee, Young-Chun (Department of Food Science and Technology, Chung-Ang University)
Publication Information
Korean Journal of Food Science and Technology / v.38, no.3, 2006 , pp. 369-377 More about this Journal
Abstract
Protopectinase (PPase) from Bacillus subtilis was used to investigate enzymatic maceration of vegetable tissues. Optimum concentration and pH of PPase were 0.75, 0.75, and 0.5%, and 5.0, 8.0, 7.0 for red pepper, garlic, and cucumber, respectively. Optimum shaking-rate, reaction time, and temperature of PPase were 250 rpm, 150 min, and $37^{\circ}C$, respectively. Yields of mechanically macerated red pepper, garlic, and cucumber were 45.8, 47.5, and 82.1%, whereas those treated with PPase were 81.8, 84, and 98%. Over 40% Vitamin C, the most unstable component during mechanical maceration, remained intact for 12 days after enzymatic treatment. Color differences $({\Delta}E)$ of mechanically macerated red pepper, garlic, and cucumber were 1.16, 2.86, and 3.27, whereas those of PPase-treated ones were 2.87, 7.68, and 5.22 after heat treatment at $100^{\circ}C$ for 20 min. Capsaicin content of mechanically macerated red pepper was 0.4 mg/100 g, whereas that treated with PPase was 1.32 mg/100 g. Viscosity of PPase-treated vegetable decreased slowly with increasing storage period, whereas that of mechanically macerated vegetable sharply decreased. These results indicate PPase treatment of vegetable could be better choice for preparation of high-values and functionally processed food and for extending preservation period.
Keywords
protopectinase; maceration; red pepper; capsaicin; single cell;
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1 Chae SK, Oh MH. Standard Food Analysis. Ji-gu Publishing. Co., Seoul, Korea pp. 536-540 (2000)
2 Park YK, Kang YH. Macerating properities of fruits and vegetables for suspensions containing single cells. Korean J. Food Sci. Technol. 36: 64-68 (2004)
3 Jang GS, Development of food processing technology. Food Technol. Ind. 30: 87.94 (1997)
4 Kim DH, Beun MW. New processing technology of traditional fermented food using radical. Food Ind. Nutr. 6: 38-44 (2001)
5 Rombouts FM, Pilnik W. Utilization of pectic enzymes in food production. Dev. Food Sci. 2: 264-268 (1979)
6 Lee SC, Hwang Yl. Recovery yields of protopectinase depending on treatments of organic solvents. Agr. Chem. Biotechnol. 40: 107-111 (1997)   과학기술학회마을
7 Park YK, Kang YH. Enzymatic maceration of vegetables with cell separating enzymes. Korean J. Postharvest. Sci. Technol. 7: 184-188 (2000)
8 Shin HH, Pyun YR. Inactivation of lactobacillus plantarum by high valtage pulsed electronic fields treatment. Korean J. Food Sci. Technol. 29: 1175-1183 (1997)
9 Biological active substances of food industry new processing technology - Focused on the membrane separation process. Food Technol. Ind. 31: 18..29 (1998)
10 Park YK, Kang YH. Effect of single cells of carrot and radish on the fecal excretion properties, mineral absorption rate and structure of small intestine and colon in rats. J. Korean Soc. Food Sci. Nutr. 33: 505-511 (2004)   DOI
11 Byun MW, Lee SH. Effect of ozone treatment and gamma irradiation on the quality properties of dried-spirullina and dried-sea, tangle powders. Korean J. Food Sci. Technol. 29: 764-770 (1997)
12 Shin JK, Byeon YL. Pasteurization of food by high intensity light pulse. Food Technol. Ind. 33: 27-35 (2000)
13 Yang IS, Lee JM, Lee YE, Yoon S. Trends and feasibility of health-oriented con venience foods of Korean food industry. Korean J. Dietary Culture 13: 215-225 (1998)   과학기술학회마을
14 Jung SJ, Kim GE, Kim SH. The changes of ascorbic acid and chlorophylls content in gochu-jangachi during fermentation. J. Korean Soc. Food Sci. Nutr. 30: 814-818 (2001)   과학기술학회마을
15 Park YK, Kang YH. Characteristics of suspension containing single cells from watermelon and muskmelon treated with cell separating enzymes. Korean J. Food Sci. Technol. 36: 58-63 (2004)
16 Lee SC, Hwang YI Effect of medium composition on protopectinase production from Bacillus subtilis EKII. Korean J. Appl. Microbiol. Biotechnol. 27: 378-384 (1999)
17 Lee SC, Hwang YI. Cell separation of vegetable tissue by protopectinase. J. Korean Soc. Food Sci. Nutr. 26: 430-435 (1997)   과학기술학회마을
18 KFDA. Food Index. Munyoung press. Korea. pp. 869-872 (2002)
19 Jung EY, Lim YH, Park MS, Kim MW. A survey of the consumption of convenience foods. Korean J. Community Nutr. 7: 149-155 (2002)
20 Lee DH, Lee SC, Hwang Yl. Processing properties of kiwifruit freated with protopectinase. J. Korean Soc. Food Sci. Nutr. 29: 410-416 (2000)
21 Vincent KA, Ken AB. Rapid sample preparation method for HPLC analysis of capsaicinoids in capsaicum fruits and oleoresions. J. Agric. Food Chem. 35: 777-779 (1987)   DOI
22 Cheigh HS, Song ES, Jeon YS. Changes of chemical and antioxidative characteristics of chlorophylls in the model system of mustard leaf kimchi during fermentation. J. Korean Soc. Food Sci. Nutr. 28: 520-525 (1999)   과학기술학회마을
23 Takashi N, Roque AH, Takuo S. Enzymatic maceration of vegetables with protopectinases. J. Food Sci. 60: 468-472 (1995)   DOI   ScienceOn