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The Investigation on the Optimum Culture Conditions and the Ice Nucleating Activity of Bacterium Xanthomonas translucens KCTC 2751  

Kim, Young-Mun (Department of Biotechnology & Bioengeering, Pukyong National University)
Kang, Sung-Il (Department of Biotechnology & Bioengeering, Pukyong National University)
Jang, Young-Boo (Department of Biotechnology & Bioengeering, Pukyong National University)
Jun, Byung-Jin (Department of Biotechnology & Bioengeering, Pukyong National University)
Kong, Jai-Yul (Department of Biotechnology & Bioengeering, Pukyong National University)
Publication Information
Korean Journal of Food Science and Technology / v.38, no.2, 2006 , pp. 249-255 More about this Journal
Abstract
The optimum culture conditions for the ice nucleating activity and the cell growth of Xanthomonas translucens KCTC 2751 were investigated. The optimum initial pH and temperature for the cell growth and the ice nucleating activity were 6.5 and $25^{\circ}C$, respectively. The optimum culture medium for the ice nucleating activity was composed of 1.0% maltose, 1.4% yeast extract, 0.8% digested of gelatin, and 0.03% KCI in distilled water. Freezing operations carried out on distilled water showed that the degrees of supercooling were $-7.90^{\circ}C$ without ice nucleators, $-1.56^{\circ}C$ with silver iodide as a commercial ice nucleator, and $-1.36^{\circ}C$ when Xanthomonas translucens KCTC 2751 were added. During progressive freeze-concentration assays, the addition of Xanthomonas translucens KCTC 2751 led to lower saccharose concentrations in the crystals, while the cells led to higher saccharose concetrations in the concentrated phase.
Keywords
ice nucleating activity; Xanthomonas translucens; optimum; supercooling; freeze-concentration;
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