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Revision of the Analysis of Makgeolli for Ochratoxin A  

Park, Je-Won (Food Research Division, Food Directorate, Health Products and Food Branch, Health Canada)
Publication Information
Korean Journal of Food Science and Technology / v.38, no.1, 2006 , pp. 140-142 More about this Journal
Abstract
Because ochratoxin A recovery level of Makgeolli is lower (<50%) than those of other commodities such as rice, barley, and beer, Makgeolli was evaluated to improve the recovery and enable routine analysis. Use of 3% sodium bicarbonate instead of phosphate-buffered saline as homogenizing solution provided good recovery of ochratoxin A (>80%) spiked in Makgeolli at level of 1 ppb. To determine if this analytical method is reliable for ochratoxin A detection in Makgeolli, survey was conducted for ochratoxin A in 30 sterile Makgeolli samples retailed in Korea. Only two wheat-based Makgeolli samples contained detectable level of ochratoxin A (0.8 and 2.1 ppb) as confirmed by HPLC- electrospray ionization- mass spectrometry. Extraction of sample with 3% sodium bicarbonate for 3 min, followed by cleanup of extracts with immunoaffinity columns, and HPLC with fluorescence detection provided dependable detection of ochratoxin A in Makgeolli samples.
Keywords
Makgeolli; ochratoxin A; HPLC-ESI-MS; HPLC-FD;
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