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Isolation and Identification of an Antioxidant Substance from Ethanol Extract of Wild Grape (Vitis coignetiea) Seed  

Kim, Nan-Young (School of biotechnology and Bioengineering, Kangwon National University)
Choi, Jae-Ho (School of biotechnology and Bioengineering, Kangwon National University)
Kim, Young-Guk (School of biotechnology and Bioengineering, Kangwon National University)
Jang, Mi-Young (Department of Food Science & Technology and Functional Food Research Center, Chonnam National University)
Moon, Jea-Hak (Department of Food Science & Technology and Functional Food Research Center, Chonnam National University)
Park, Geun-Hyung (Department of Food Science & Technology and Functional Food Research Center, Chonnam National University)
Oh, Deog-Hwan (School of biotechnology and Bioengineering, Kangwon National University)
Publication Information
Korean Journal of Food Science and Technology / v.38, no.1, 2006 , pp. 109-113 More about this Journal
Abstract
Antioxidant compound(s) were identified from the ethanol extract of wild grape (Vitis coignetiea) seed. Organic solvent fractions of n-hexane, chloroform, ethyl acetate and butanol were obtained from the ethanol extract of wild grape seed, among which ethyl acetate fraction showed the strongest reducing power. Ethyl acetate fraction was further purified through ODS column chromatography and HPLC, and isolated antioxidative active compound was identified through $^1H-NMR$ as (+)-catechin (52.7 g/100 g). (+)-Catechin and ethyl acetate fraction both showed approximately 80% scavenging effect. These results indicated (+)-catechin in the ethyl acetate fraction synergetically interacts with unknown antioxidative compound(s).
Keywords
wild grape seed; Vitis coignetiea; antioxidant; reducing power; (+)-catechin;
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Times Cited By KSCI : 3  (Citation Analysis)
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