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Changes in Microorganisms and Enzyme Activities of Low-salted Kochujang added with Horseradish Powder during Fermentation  

Oh, Ji-Young (R&D Center, Doosan Co.)
Kim, Yong-Suk (Research Center for Industrial Development of BioFood Materials, Chonbuk National University)
Shin, Dong-Hwa (Faculty of Biotechnology (Food Science & Technology), Chonbuk National University)
Publication Information
Korean Journal of Food Science and Technology / v.37, no.3, 2005 , pp. 463-467 More about this Journal
Abstract
To reduce salt content in Korean traditional kochujang, horseradish powder (1.2%, w/w) was added to kochujang with 4-10% salt, and its microbial characteristics, enzyme activities, and gas formation in kochujang were evaluated during fermentation far 120 days at $25^{\circ}C$. All treatments of kochujang had no effects on total viable bacterial numbers, which kept constant level, during fermentation (7.32-8.765 log CFU/g). Yeast numbers did not change under all treatments up to 90 days of fermentation, then decreased thereafter, ${\alpha}$-Amylase and ${\beta}$-amylase, and neutral- and acid-pretense activities of kochujang added with horseradish powder were higher than those of control group. ${\beta}$-Amylase activity of kochujang increased in proportion to salt concentration. Total accumulative volume of gas produced during fermentation of kochujang without horseradish powder was 5,892 mL/pack then decreased to 121-347mL/pack with low-salted kochujang (salt 4%, 6%) added with horseradish powder, Major gas produced was $CO_{2}(74-80%)$. Results indicate salt contents of kochujang could be lowered up to 6% by addition of horseradish powder without gas formation and quality alteration.
Keywords
kochujang; horseradish; gas formation; ${\alpha}$- and ${\beta}$-amylase; low-salt;
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1 Shin DH, Ahn EY, Kim YS, Oh JY. Changes in the microflora and enzyme activities of kochujang prepared with different koji during fermentation. Korean J. Food Sci. Technol. 33: 94-99 (2001)
2 Arlene WC, Rena RW, Mary PN, Milas NC, Seung L, Herbert L. The measurement of sodium and potassium in take. Am. J. Clin. Nutr. 42: 391-398 (1985)   DOI   PUBMED
3 Shin DH, Kim DH, Choi U, Lim DK, Lim MS. Studies on the physicochemical characteristics of traditional kochujang. Korean J. Food Sci. Technol. 28: 157-161 (1996)
4 Jung SW, Kim YH, Koo MS, Shin DH, Chung KS, Kim YS. Change in physicochemical properties of industry-type kochujang during storage. Korean J. Food Sci. Technol. 26: 403-410 (1994)
5 Kim GT, Hwang YI, Lim SI, Lee DS. Carbon dioxide production and quality changes in Korean fermented soybean paste and hot pepper-soybean paste. J. Korean Soc. Food Sci. Nutr. 29: 807-813 (2000)
6 Kim MS, Ahn YS, Shin DH. Analysis of browning factors during fermentation of kochujang. Korean J. Food Sci. Technol. 32: 1149-1157(2000)
7 Shin DH, Ahn EY, Kim YS, Oh JY. Fermentation characteristics of kochujang containing horseradish or mustard. Korean J. Food Sci. Technol. 32: 1350-1357 (2000)
8 Ahn ES, Kim JH, Shin DH. Antimicrobial effects of allyl isothio-cyanates on several microorganisms. Korean J. Food Sci. Technol. 31: 206-211 (1999)
9 Seo KL, Kim DY, Yang SL. Studies on the antimicrobial effect of wasabi extracts. Korean J. Nutr. 28:1073-1077 (1995)
10 Lee KS, Kim DH, Moon CO. Effect of ethanol and lactic acid on the preparation of low salted kochujang. Theses Collection of Wonkwang Univ., Iksan, Korea. 20: 143-164 (1986)
11 Kim KH, Bae JS, Lee TS. Studies on the quality of kochujang prepared with grain and flour of glutinous rice. J. Korean Soc. Agric. Chem. Biotechnol. 29: 227-236 (1986)
12 Oh HI, Park JM. Changes in microflora and enzyme activities of traditional kochujang prepared with a meju of different fermentation period during aging. Korean J. Food Sci. Technol. 29: 1158-1165(1997)
13 Kim MS, Kim IW, Oh JA, Shin DH. Effect of different koji and irradiation on the quality of traditional kochujang. Korean J. Food Sci. Technol. 31: 196-205 (1999)
14 Lee KH, Lee MS, Park SO. Studies on the microflora and enzymes influencing on Korea native kochujang (red pepper soybean paste) aging. J. Korean Agric. Chem. Soc. 19: 82-92 (1976)
15 Lee KS, Kim DH. Trial manufacture of low-salted kochujang (red pepper soybean paste) by the addition of alcohol. Korean J. Food Sci. Technol. 17: 146-154 (1985)
16 Kim YS, Kwon DJ, Koo MS, Oh HI, Kang TS. Changes in microflora and enzyme activities of traditional kochujang during fermentation. Korean J. Food Sci. Technol. 25: 502-509 (1993)
17 Lee KS, Kim DH. Effect Bacillus subtilis on the quality of the low salted kochujang. Thesis Collection of Wonkwang Univ., Iksan, Korea. 23: 431-447 (1989)
18 Dahl LK. Salt and hypertension. Am. J. Clin. Nutr. 25: 231-244 (1972)   DOI   PUBMED
19 Lee KY, Kim HS, Lee HG, Han O, Chang UJ. Studies on the prediction of the shelf-life of kochujang through the physico-chemical and sensory analyses during storage. J. Korean Soc. Food Sci. Nutr. 26: 588-594 (1997)
20 Kim JO, Lee KH. Effect of temperature on color and color-preference of industry-produced kochujang during storage. J. Korean Soc. Food Sci. Nutr. 23: 641-646 (1994)
21 Lee JS, Choi YJ, Kwon SJ, Yoo JY, Chung DH. Screening and characterization of osmotolerant and gas-producing yeasts from traditional doenjang and kochujang. Food Sci. Biotechnol. 5: 54-58(1996)
22 Nam HW, Lee GY. A study on the sodium and potassium intake and their metabolism of the pregnant women in the Korea. Korean J. Nutr. 18:194-200 (1985)
23 Cho HO, Pack SA, Kim JG. Effect of traditional and improved kochujang koji on the quality improvement of traditional kochujang. Korean J. Food Sci. Technol. 13: 319-327 (1981)
24 Choi KS, Chung YG, Choi C, Chung HC, Im MH, Choi JD, Lee CW. Lactic acid and alcoholic fermentation of low-slated raw kochujang digestion liquor made from Bacillus subtilis var. glo-bigii and Scopulariopsis brevicaulis inoculated meju. Agric. Chem. Biotechnol. 41: 405-409 (1998)
25 Kim YS. Studies on the physicochemical characteristics and volatile compounds of traditional kochujang during fermentation. PhD thesis, University of Sejong, Seoul, Korea (1993)
26 Pack JM, Oh HI. Changes in microflora and enzyme activities of traditional kochujang meju during fermentation. Korean J. Food Sci. Technol. 27: 56-62 (1995)
27 Shin DH, Kim DH, Choi U, Lim MS, Ahn EY. Changes in microflora and enzymes activity of traditional kochujang prepared with various raw materials. Korean J. Food Sci. Technol. 29: 901-906(1997)
28 Oh JY, Kim YS, Shin DH. Changes in physicochemical characteristics of low-salted kochujang with natural preservatives during fermentation. Korean J. Food Sci. Technol. 34: 835-841 (2002)
29 MOHW. '95 National Nutrition Survey Report. Ministry of Health and Welfare, Seoul, Korea, pp. 46-47 (1997)
30 Seo HJ, Jeong SH. Inhibitory effect of kochujang on angiotensin converting enzyme. Bull. Korean Diet. Cult. pp. 143-157 (1997)
31 Shofran BG, Purrington ST, Breidt F, Fleming HP. Antimicrobial properties of sinigrin and its hydrolysis products. J. Food Sci. 63: 621-624(1998)
32 Shin DB, Park WM, Yi OS, Koo MS, Chung KS. Effect of storage temperature on the physicochemical characteristics in kochujang (red pepper soybean paste). Korean J. Food Sci. Technol. 26:300-304(1994)
33 Korean Recommended Dietary Allowance. 6th ed. Korean Society of Nutrition, Seoul, Korea, p. 14 (1994)
34 Jeong DY, Song MR, Shin DH. Prevention of swelling and quality improvement of Sunchang traditional kochujang by natural additives. J. Korean Soc. Food Sci. Nutr. 30: 605-610 (2001)