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Genotypic and Phenotypic Characteristics of Staphylococcus aureus Isolates from Lettuces and Raw Milk  

Jung, Hye-Jin (Department of Food Science and Technology, Chung-Ang University)
Cho, Joon-Il (Department of Food Science and Technology, Chung-Ang University)
Park, Sung-Hee (Department of Food Science and Technology, Chung-Ang University)
Ha, Sang-Do (Department of Food Science and Technology, Chung-Ang University)
Lee, Kyu-Ho (Department of Environmental Science, Hankuk University of Foreign Studies)
Kim, Cheol-Ho (Department of Biochemistry and Molecular Biology, College of Oriental Medicine, Dongguk University)
Song, Eun-Seop (Department of Obstetrics and Gynecology, College of Medicine, Inha University)
Chung, Duck-Hwa (Division of Applied Life Science, Gyeongsang National University)
Kim, Min-Gon (Laboratory of Integrative Biotechnology, Korea research Institute of Bioscience and Biotechnology)
Kim, Kwang-Yup (Department of Food Science and Technology, Chungbuk National University)
Kim, Keun-Sung (Department of Food Science and Technology, Chung-Ang University)
Publication Information
Korean Journal of Food Science and Technology / v.37, no.1, 2005 , pp. 134-141 More about this Journal
Abstract
To characterize genotypic and phenotypic traits of Staphylococcus aureus isolates (n = 86) from lettuces and raw milk, major virulence-associated genes and antibiotic susceptibility were detected using PCR-based methods and disk diffusion method, respectively. All isolates possessed coagulase gene and showed five polymorphism types [500 bp (2.4%), 580 bp (17.4%), 660 bp (61.6%), 740 bp (17.4%), and 820 bp (1.2%)] due to variable numbers of tandem repeats present within the gene. Two or three different loci of hemolysin gene family were dominant in isolates, 47 of which (55%) possessed combination of hla/hld/hlg-2 genes as the most prevalent types. Among enterotoxin-encoding genes, sea was detected from 32 isolates (37%), sed from 1 isolate (1%), and sea and sed genes were co-detected from 4 isolates (5%), whereas seb, sec, and tsst-1 genes were not detected. All isolates were susceptible to ciprofloxacin, trimethoprim/sulfamethoxazole, oxacillin, and vancomycin, 85 isolates (99%) to penicillin G, 54 isolates (63%) to chloramphenicol, 51 isolates (59%) to erythromycin, and 7 isolates (8%) to clindamycin. Among resistant isolates, seven displayed multiantibiotic-resistance against two different antibiotics.
Keywords
Staphylococcus aureus; coagulase; hemolysin; enterotoxin; antimicrobial susceptibility;
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