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Purification of Eicosapentaenoic Acid (EPA) and Docosahexaenoic Acid (DHA) Esters from Squid Oil by Silver Ion Chromatography  

Gyoung, Young-Soo (Department of Chemistry, Kangnung National University)
Yu, Ying-Lian (Department of Food Science, Kangnung National University)
Yoon, Jung-Ro (Department of Food Science, Kangnung National University)
Publication Information
Korean Journal of Food Science and Technology / v.36, no.2, 2004 , pp. 361-364 More about this Journal
Abstract
EPA and DHA extracted from methyl esterified squid oil were purified by silver exchanged resin, silver nitrate-impregnated silica gel, silver exchanged zeolite, and silica gel column chromatography, among which column chromatography using mixture of silver exchanged resin and silica gel (10% by weight) showed the best result. By this simple purification method, EPA and DHA were concentrated from 12.5 to 27.9% (yield, 86,0%) and from 21.7 to 49.5% (yield, 87.3%), respectively. Silver exchanged resin had additional advantages of outstanding reusability and simple recovery of silver.
Keywords
silver exchanged resin; EPA; DHA; purification;
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