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Antioxidant Effect of Rosa davurica Pall Extract on Oxidation of Human Low Density Lipoprotein (LDL)  

Sa, Jae-Hoon (Gangwon Research Institute of Health and Environment)
Lee, Woan (Gangwon Research Institute of Health and Environment)
Shin, In-Cheol (Gangwon Research Institute of Health and Environment)
Jeong, Kyung-Jin (Gangwon Research Institute of Health and Environment)
Shim, Tae-Heum (Gangwon Research Institute of Health and Environment)
Oh, Heung-Seok (Gangwon Research Institute of Health and Environment)
Kim, Yong-Jin (Gangwon Research Institute of Health and Environment)
Cheung, Eui-Ho (Gangwon Research Institute of Health and Environment)
Kim, Gwang-Gee (Jungsun Agriculture Technology Center)
Choi, Dae-Sung (Jungsun Agriculture Technology Center)
Publication Information
Korean Journal of Food Science and Technology / v.36, no.2, 2004 , pp. 311-316 More about this Journal
Abstract
Antioxidant effects of Rosa davurica Pall extract on copper-mediated LDL oxidative modification were investigated. Oxidation products of LDL were determined based on TBA value, formation of conjugate diene, and apolipoprotein carbonyl value. As revealed through TBA values, ethyl acetate and butanol fractions of R. davurica Pall root showed strong antioxidant effect, with 85.3 and 93.2% inhibitions at $30\;{\mu}g/mL$ each, respectively. Ethyl acetate and butanol fractions at $30\;{\mu}g/mL$ inhibited LDL oxidation up to 8 hr. Conjugate diene formation by lipid oxidation with $Cu^{2+}$ addition in ethyl acetate and butanol fractions decreased 2.2-and 5.6-fold, respectively, compared to control. Carbonyl value decreased in the presence of butanol and ethyl acetate fractions. Methanol and ethyl acetate extracts of R. davurica Pall root showed higher absorbancy at 285 nm. Ethanol extract of R. davurica Pall root and stem contained 10.6 g/100 g total phenolic compounds. Results reveal phenolic compound as major biological component in R. davurica Pall extracts. Ethyl acetate and butanol fraction showed strongest antioxidant effect on LDL oxidation.
Keywords
low-density lipoprotein(LDL); antioxidants; Rosa davurica Pall; phenolic compounds;
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