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A Thermostable Protease Produced from Bacillus sp. DF 218  

Lee, Joung-Hee (Department of Food Engineering, Dankook University)
Bai, Dong-Hoon (Department of Food Engineering, Dankook University)
Publication Information
Korean Journal of Food Science and Technology / v.36, no.1, 2004 , pp. 105-110 More about this Journal
Abstract
Microorganism (strain DF 218) producing thermostable pretense was isolated from Korean soil and compost. It was Gram-positive, rod-shaped, aerobic, and spore-forming with yellowish white colony color, Temperature range for growth at pH 6.5 was $30-65^{\circ}C$, with optimum growth at $60^{\circ}C$. pH range for growth at $60^{\circ}C$ was 5-7 with optimum of 6.5, which indicates strain DF 218 to be thermophilic. The 16S rDNA sequence of strain DF 218 had 95% sequence similarity with that of Bacillus flexus. Based on physiological properties and phylogenetic analysis, we proposed the isolated strain as Bacillus sp. DF 218. Pretense was produced aerobically at $60^{\circ}C$ for 32 hr in a medium (pH 6.5) containing 1% each trypton, glucose, and NaCl. Its molecular weight was estimated as 61 kDa, with optimum temperature and pH of $60^{\circ}C$ and 7.5, respectively.
Keywords
thermostable microorganism; protease;
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