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Changes of Saponin during the Cultivation of Soybean Sprout  

Oh, Bong-Yun (Food Industrial Technology Research Center, Mokpo National University)
Park, Bock-Hee (Food Industrial Technology Research Center, Mokpo National University)
Ham, Kyung-Sik (Food Industrial Technology Research Center, Mokpo National University)
Publication Information
Korean Journal of Food Science and Technology / v.35, no.6, 2003 , pp. 1039-1044 More about this Journal
Abstract
We investigated the changes in saponins during the cultivation of soybean sprout. Crude saponin content was 4.15mg/g in germinated soybean and reached its peark (5.33mg/g) in soybean sprout cultivated for six days. Saponin content in the cotyledon, stem, and root of the soybean sprout cultivated for six days were 4.17, 7.46, and 7.45mg/g, respectively. Soyasaponins extracted from the soybean sprout were analyzed with LC-electrospray ionization (ESI)-mass spectrometry, in which a reverse phase $C_18$ column was used for separation of saponins. In the soybeen sprout, group B saponin, I, II, III, IV, and V increased 7, 2, 1.4, 8.7, and 3.3 fold, respectively, compared to those in the soybean seed. Group B saponin I, II, III, IV, and V in the stem of the soybean sprout were 10.53, 1.45, 10.49, 5.72 and 8.14 fold the level of those in the cotyldon, respectively. In the root, the contents of group B saponin I, III, IV, and V were 5.54, 2.77, 4.86 and 9.73 fold, respectively, higher than those in cotyledon, but the content of group B saponin 2 was 2.96 fold less than that in cotyledon. These results indicate that the biosyntheses of group B saponins are differentially regulated in growing soybean sprout.
Keywords
soybean sprout; saponin; soyasaponin group B; LC/MS;
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