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Isolation and Identification of Quercetin with Antioxidative Activity from the Fruits of Rubus coreanum Miquel  

Yoon, In (Department of Food Science and Technology and Institute of Agricultural Science and Technology, Chonnam National University)
Wee, Ji-Hyang (Department of Food Science and Technology and Institute of Agricultural Science and Technology, Chonnam National University)
Moon, Jae-Hak (Department of Applied Biology and Chemistry, The Research Institute of Agricultural Resources Development, Konkuk University)
Ahn, Tae-Hoe (Research Center, Ottogi Food Co., Ltd.)
Park, Keun-Hyung (Department of Food Science and Technology and Institute of Agricultural Science and Technology, Chonnam National University)
Publication Information
Korean Journal of Food Science and Technology / v.35, no.3, 2003 , pp. 499-502 More about this Journal
Abstract
The methanol (MeOH) extracts from the fruits of Rubus coreanum showed antioxidative activity. The antioxidative substance in MeOH extracts was successively purified with solvent fractionation, adsorption chromatography, and gel filtration. The purified active substance was isolated by HPLC and identified as quercetin by EI-MS, and $^1H-NMR$ analyses. The amount of quercetin was 0.25 mg per 100 g in fresh fruits of Rubus coreanum Miquel.
Keywords
fruits of Rubus coreanum Miquel; antioxidative activity; quercetin;
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1 Halliwell, B.H., Gutteridge, J.M.C. and Arouoma, O.I. The deoxyribose method: A simple 'test-tube' assay for determination of rate constants for reaction of hydroxyl radicals. Anal. Biochem. 165: 215-219 (1987)   DOI   ScienceOn
2 Branen, A.L. Toxicological and biochemistry of butylated hydroxytoluene, butylated hydroxyanisole. J. Am. Oil Chem. Soc. 52: 59-63 (1975)   DOI   PUBMED
3 Kim, T.J. Korean Resources Plants II, p. 140. Publishing Department of Seoul University, Seoul, Korea (1996)
4 Lee, Y.A. and Lee, M.W. Tannins from Rubus coreanum. Korean J. Pharmacogn. 26: 27-30 (1995)
5 Kim, M.S., Pang, G.C. and Lee, M.W. Flavonoids from the leaves of Rubus coreanum. Yakhak Hoeji 41: 1-6 (1997)
6 Pang, G.C., Kim, M.S. and Lee, M.W. Hydrolyzable tannins from the fruits of Rubus coreanum. Korean J. Pharmacogn. 27: 366-370 (1996)
7 Abe, N., Nemoto, A., Tsuchiya, Y., Hojo, H. and Hirota, A. Studies of the 1,1-diphenyl-2-picrylhydrazyl radical scavenging mechanism for a 2-pyrone compound. Bioscl. Biotech. Biochem. 64: 306-333 (2000)   DOI   ScienceOn
8 Peng, Z.F., Strack, D., Baumert, A., Subramaniam, R., Goh, K.N., Chia, T.F., Tan, S.N. and Chia, L.S. Antioxidant flavonoids from leaves of Polygonum hydropiper L. Phytochemistry 62: 219-228 (2003)   DOI   ScienceOn
9 Markaverich, B.M., Roberts, R.R., Alejandro, M.A., Johnan, G.A., Middleditch, B.S. and Clark, J.M. Bioflavonoid interaction with rat utenne type II binding sites and growth inhibition. J. Steroid Biochem. 30: 71-78 (1998)   DOI   ScienceOn
10 Veckenstedt, A., Beladi, I. and Musci, I. Effect of treatment with certain tlavonoids on mengo virus-induced encephalitis in mice. Arch. Virol. 57: 255-260 (1978)   DOI   ScienceOn
11 Takao, T., Kitatani, F. and Sakata, K. A simple screening method for antioxidants and isolation of several antioxidants produced by marine bacteria from fish and shellfish. Biosci. Biotech. Biochem. 58: 1780-1783 (1994)   DOI
12 Kuk, J.H., Ma, S.J., Moon, J.H., Kim, K.Y., Choi, S.H. and Park, K.H. Antibacterial and antifungal activities of a naphthoquinone derivative isolated from the fruits of Catalpa ovata G. D$_O_N$. J. Microbial. Biotechnol. 12: 858-863 (2002)
13 William, M., Amanda, J.S., Michael, E.J.L., Peter, G., Garry, G.D. and Alan, C. Effect of freezing and storage on the phenolics, ellagitannins, flavonoids, and antioxidant capacity of red raspberries. J. Agric. Food Chem. 50: 5197-5201 (1999)   DOI   ScienceOn
14 Hakkinen, S.H., Karenlampi, S.O., Heinonen, I.M., Mykkanen, H.M. and Torronen, A.R. Content of the flavonols quercetin, myricetin, and kaempferol in 25 edible berries. J. Agric. Food Chem. 47: 2274-2279 (1999)   DOI   ScienceOn
15 Moon, J.H. and Park, K.H. Functional components and physiological activity of tea. J. Korean Tea Soc. 1: 175-191 (1995)
16 Gutteridge, J.M.C. and Halliwell, B. Antioxidants in Nutrition, Health and Disease, pp. 1-62. Oxford University Press, UK (1994)
17 Park, K.H., Park, J.D., Hyun, K.H., Nakayama, M. and Yokota, T. Brassinosteroids and monoglycerides with brassinosteroid-like activity in immature seeds of Oryza sativa and Perilla frutescena and in cultured cells of Nicotiana tabacum. Bioscl. Biotech. Biochem. 58: 224-2243 (1994)
18 Halliwell, B.H. and Gutteridge, J.M.C. Role of free radical and catalytic metal ions in human disease: An overview. Methods Enzymol. 186: 1-85 (1990)   DOI   PUBMED
19 Ramarathnam, N., Osawa, T., Ochi, H. and Kawakishi, S. The contribution of plant food antioxidants to human health. Trends Food Sci. 6: 75-82 (1995)   DOI   ScienceOn
20 Lu, Y. and Foo, L.Y. Identification and quantification of major polyphenols in apple pomace. Food Chem. 59: 187-194 (1997)   DOI   ScienceOn
21 Younes, M. and Siegers, C.P. Inhibitory action of some tlavonoids on enhanced spontaneous lipid peroxidation fellowing glutathione depletion. Planta Medica. 43: 240-244 (1981)   DOI   ScienceOn
22 Edenhader, R. and Tang, X. Inhibition of the mutagenicity of 2-nitrotluorene, 3-nitrotluoranthene and I-nitropyrene by flavonoids, coumarins, quinones and other phenolic compound. Food Chern. Toxicol. 35: 357-372 (1996)
23 Moon, H.I., Ahn, K.T., Lee, K.R. and Zee, O.P. Flavonoid compounds and biological activities on the aerial parts of Angelica gigas. Yakhak Hoeji 44: 119-127 (2000)
24 Lee, M.W. Phenolic compounds from the leaves of Rubus coreanum. Yakhak Hoeji 39: 200-204 (1995)
25 Lee, S.T., Lee, Y.H., Choi, Y.J., Shon, G.M., Lee, H.J. and Heo, H.S. Comparison of quercetin and soluble tannin in Houttuynia cordata THUNB. according to growth stages and plants parts. Korean J. Med. Crop Sci. 10: 12-16 (2002)
26 Ham, I.H., Lee, S.J., Kim, H.H., Kang, I.H., Jin, H.O. and Whang, W.K. Standardization and seasonal variation of quercetin glycoside in Eucommiae Folium. Korean J. Phannacogn. 33: 194-199 (2002)
27 Wee, J.H. and Park, K.H. Isolation of 4-hydroxycinnamic acid, 3methoxy- 4-hydroxycinnamic acid and 3,4-dihydroxybenzoic acid with antioxidative and antimicrobial activity from peanut (Arachis hypogaea) shell. Food Sci. Biotechnol, 10: 551-556 (2001)
28 Formica, J.V. and Regelson, W. Review of the biology of quercetin and related bioflavonoids. Food Chem. Toxicol. 33: 1061-1080 (1995)   DOI   ScienceOn
29 Cho, J.Y., Moon, J.H. and Park, K.H. Isolation and identification of 3-methoxy-4-hydroxybenzoic acid and 3-methoxy-4-hydroxycinnamic acid with antioxidative and antimicrobial activity from hot water extracts of Hovenia dulcis Thunb and conformation of their antioxidative and antimicrobial activity. Korean J. Food Sci. Technol. 32: 1403-1408 (2000)
30 Gadow, A.V., Joubert, E. and Hansmann, C.F. Comparison of the antioxidant activity of aspalathin with that of other plant phenols of rooibos tea (Aspalathus linearis), $\alpha$-tocopherol, BHT, and BHA. J. Agric. Food Chem. 45: 632-638 (1997)   DOI   ScienceOn
31 Yoon, I., Cho, J.Y., Kuk, J.H., Wee, J.H., Jang, M.Y., Ahn, T.H. and Park, K.H. Identification and activity of antioxidative compounds from Rubus coreanum fruit. Korean J. Food Sci. Technol. 34: 898-904 (2002)