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Characterization of Squalene Synthase Inhibitor Isolated from Curcuma longa  

Choi, Sung-Won (Research Institute of Biotechnology, Green Biotech Co. Ltd.)
Yang, Jae-Sung (Research Institute of Biotechnology, Green Biotech Co. Ltd.)
Lee, Han-Seung (Directed Evolution Team, GenoFocus Inc.)
Kim, Dong-Seob (Department of Food Science, Miryang National University)
Bai, Dong-Hoon (Department of Food Engineering, Dankook University)
Yu, Ju-Hyun (Korean Culture Center of Microorganisms)
Publication Information
Korean Journal of Food Science and Technology / v.35, no.2, 2003 , pp. 297-301 More about this Journal
Abstract
An inhibitor of squalene synthase, a key enzyme in the cholesterol biosynthetic pathways and a target for improved agents to lower plasma levels of low-density lipoprotein, was sequentially purified from Curcuma longa by acetone extraction, silica gel column chromatography, and sephadex LH-20 column chromatography. Active compound, YUF-01, was successfully purified and analyzed as $C_{20}H_{21}O_6$ by electron ionization mass spectrum. Through $^1H-NMR$ and $^{13}C-NMR$ analyses, YUF-01 was identified as curcumin, which showed strong inhibition of squalene synthase.
Keywords
curcumin; Curcuma longa; cholesterol biosynthesis; squalene synthase; inhibitor;
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