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Effects of Grape Extracts on Free Radical Scavenging Activity and Inhibition of Pro-Inflammatory Mediator Production in Mouse Macrophage Cells  

Min, Hye-Young (College of Pharmacy, Ewha Womans University)
Park, Eun-Jung (College of Pharmacy, Ewha Womans University)
Lee, Sang-Kook (College of Pharmacy, Ewha Womans University)
Cho, Yong-Jin (Korea Food Research Institute)
Publication Information
Korean Journal of Food Science and Technology / v.35, no.1, 2003 , pp. 132-137 More about this Journal
Abstract
Antioxidant and anti-inflammatory potentials of various grape extracts were evaluated. Extracts from Kyho seed, Kyho stem, and Campbell seed showed potent 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activities compared to resveratrol $(IC_{50}=16.9,\;21.5,\;21.9,\;34.6\;{\mu}g/mL,\;respectively)$, among which, antioxidant effect of Kyho seed extract were similar to that of vitamin C $(IC_{50}=12.2\;{\mu}g/mL)$. These extracts also exhibited inhibitory activities on lipopolysaccharide (LPS)-induced prostaglandin $E_2$ production and nitrite formation in mouse macrophage RAW 264.7 cells at $50\;{\mu}g/mL$. Kyho stem and seed extracts showed growth inhibitory activities in human lung and colon cancer cells. These results suggest the potential roles of grape extracts as antioxidants and anti-inflammatory agents.
Keywords
grape extracts; free radical scavenging; prostaglandins; iNOS; mouse macrophage cells;
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Times Cited By KSCI : 1  (Citation Analysis)
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