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http://dx.doi.org/10.15269/JKSOEH.2019.29.4.508

Acute Toxicity Study of the Glyoxal by Intratracheal Instillation in Male Sprague-Dawley Rats  

Kim, Hyeon-Young (National Center for Efficacy Evaluation of Respiratory Disease Product, Korea Institute of Toxicology)
Kim, Kicheon (National Center for Efficacy Evaluation of Respiratory Disease Product, Korea Institute of Toxicology)
Kim, In-Hyeon (National Center for Efficacy Evaluation of Respiratory Disease Product, Korea Institute of Toxicology)
Kim, Min-Seok (National Center for Efficacy Evaluation of Respiratory Disease Product, Korea Institute of Toxicology)
Kim, Sung-Hwan (National Center for Efficacy Evaluation of Respiratory Disease Product, Korea Institute of Toxicology)
Lee, Kyuhong (National Center for Efficacy Evaluation of Respiratory Disease Product, Korea Institute of Toxicology)
Publication Information
Journal of Korean Society of Occupational and Environmental Hygiene / v.29, no.4, 2019 , pp. 508-516 More about this Journal
Abstract
Objectives: The present study was performed to obtain acute toxicity information on glyoxal in male rats after intratracheal instillation. Methods: In order to calculate the LD50 of glyoxal using Probit analysis with SAS, the test article was one intratracheal instillation to male Sprague-Dawley rats at dose levels of 0, 225, 451 or 902 mg/kg. During the test period, mortality, clinical signs, and body and organ weights were examined. At the end of the 14-day observation period, all animals were sacrificed and complete gross postmortem and histopathological examinations were performed. Results: Four animals of the 902 mg/kg group died within one week after the administration of glyoxal. All treatment group in a dose dependent manner, decreased body weight was found during the study period. The absolute and relative lung weight, and histopathological changes (bronchiolar-alveolar hyperplasia, chronic inflammation) of lung exhibited an increased in glyoxal treated groups in a dose dependent manner. However, there were no changes on the organ weights and histopathological changes of any other organ except lung. Conclusions: The results obtained in the present study suggest that the LD50 in male Sprague-Dawley rats after a single intratracheal instillation of glyoxal was considered to be 866.9 mg/kg and the lung was found to be the target organ for glyoxal.
Keywords
acute toxicity; glyoxal; intratracheal instillation; $LD_{50}$; pulmonary toxicity;
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