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Cytotoxicity and DNA Damage in Human Lung Cells Treated with Lanthanum Oxide and Neodymium Oxide  

Kim, Jong Kyu (Toxicity Research Team, Center for Chemicals Safety and Health, Occupational Safety & Health Research Institute)
Kim, Soo Jin (Toxicity Research Team, Center for Chemicals Safety and Health, Occupational Safety & Health Research Institute)
Kang, Min Gu (Toxicity Research Team, Center for Chemicals Safety and Health, Occupational Safety & Health Research Institute)
Chung, Young Hyun (Toxicity Research Team, Center for Chemicals Safety and Health, Occupational Safety & Health Research Institute)
Publication Information
Journal of Korean Society of Occupational and Environmental Hygiene / v.23, no.2, 2013 , pp. 50-56 More about this Journal
Abstract
Objectives: The present study investigated cytotoxicity and DNA damage in human lung cells in vitro. Methods: Neodymium oxide and lanthanum oxide were dispersed by ultrasonic treatments. The assay was performed with MRC-5 (Human male fetus lung cell). Cytotoxicity and comet assay of lanthanum oxide and neodymium oxide were measured after 24 and 48 hours incubation. Results: After 24 hours of exposure to rare earth metals, the cytotoxicities of lanthanum oxide in more than $1{\mu}M$ concentration groups were significantly increased when compared to the control group, but the cytotoxicities of neodymiun oxide in more than $100{\mu}M$ concentration groups were statistically increased. After 48 hours exposure, cytotoxicities of both materials were statistically increased in $100,000{\mu}M$ concentration groups. Olive tail moments of the lanthanum oxide treated group were significantly increased when compared to the control group. Conclusions: The cytotoxicity of lanthanum oxide was higher than that of neodymium oxide. The DNA of MRC-5 cells treated with lanthanum oxide for 48 hours were significantly damaged.
Keywords
rare earth metals(REM); cell toxicity; Comet assay; MRC-5;
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