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Expression of cytochrome P-450(CYP)2B1/2 in lymphocytes of workers exposed to toluene and xylene  

Kim, Ki-Woong (Occupational Safety and Health Research Institute, KOSHA)
Publication Information
Journal of Korean Society of Occupational and Environmental Hygiene / v.21, no.1, 2011 , pp. 49-54 More about this Journal
Abstract
In order to develop the methods for exposure assessment and find susceptibility markers for the workers who are exposed to low doses of toluene, xylene and other chemical in petroleum industries, we investigated the application of P-450 expression in human lymphocytes utilizing mouse monoclonal anti-rat CYP2B1/2, the levels of toluene and xylene in air and their metabolite levels in urine with the levels of expressed CYP2B1/2 proteins. The general characteristics such as age, smoking and drinking habit were no significant difference between the control and exposed workers, but the working durations and working hours were significantly different. Workers in exposed group were exposed to the mean of 2.1 ppm (range, 0.00-4.54) of toluene and 0.3 ppm (rang, 0.00-1.23) of xylene. The mean concentration of urinary hippuric acid was low and less than 1/5 of the biological exposure index recommended by the Ministry of Employment and Labor Korea. Methyl hippuric acid in urine was not detected in control and exposed workers. Also, there were no significant differences in the levels of the urinary metabolites between the control and exposed group. When chemiluminescence dot blottings were carried out utilizing mouse monoclonal antibody against CYP2B1/2, the strong density dots corresponding to a mouse monoclonal antibody was observed in the human lymphocytes from the exposed workers. These results suggested that the chemiluminescence dot blot assay for CYP of lymphocytes should be valuable for identifying CYP expression as biomarkers in the workers exposed to toluene and xylene.
Keywords
Toluene; Xylene; Human lymphocytes; CYP2B1/2; Metabolites;
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Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 고용노동부. 산업안전보건법령집. 2009
2 김기웅, 허경화, 원용림, 정진욱, 김태균, 박인정. Styrene 노출에 반응을 보이는 혈청 단백질에 대한 프로테오믹스 분석. 한국산업위생학회지2007;17(3):235-244
3 김기웅, 허경화. Xylene에 의한 CYP2B1/2의 유도와 대사에 있어서 toluene의 영향. 한국산업위생학회지 2009;19(1):73-79
4 배기택, 문덕환, 김종한, 문찬석, 이채언. 톨루엔 크실렌 및 벤젠폭로의 생화학적지표들에 관한 연구. 대한산업의학회지 1991;3(2):165-176
5 Arlien-S?borg P. Solvent neurotoxicity. CRC Press Inc., Florida, 1992, 107-124
6 Carvalho D, Lanchote VL, Bonato PS, Queiroz R, Santos AC, and Dreossi S. A new derivatization procedure for the analysis of hippuric acid and methyl hippuric acid by gaschromatography. Int Arch Occup Environ Health 1991;63:33-37   DOI   ScienceOn
7 Chung W-G. Roh H-K, Kim H-M, Cha Y-N. Involvement of CYP3A1, 2B1, and 2E1 in C-8 hydroxylation and CYP1A2 and flavin-containing monooxygenase in N-demethylation of caffeine: identified by using inducer treated rat liver microsomes that are characterized with testosterone metabolic patterns. Chemico-Biological Interactions 1998;113:1-14   DOI   ScienceOn
8 Cohr KH, Stokholm J. Toluene. a toxicology review. Scand J Work Environ Health 1979;5:71-90   DOI   ScienceOn
9 DeMarini DM. Genotoxicity of tobacco smoke and tobacco smoke condensate: a review. Mutation Res 2004;567:447-474   DOI   ScienceOn
10 Elovaara E, Stockmann-Juvala H, Mikkola J, Gelboin HV. Interactive effects of methyl tertiary-butyl ether (MTBE) and tertiary-amyl methyl ether (TAME), ethanol and some drugs: Triglyceridemia, liver toxicity and induction of CYP (2E1, 2B1) and phase II enzymes in female Wistar rats. Environ Toxicol Pharmacol 2007;23:64-72   DOI   ScienceOn
11 Foy JW, Schatz RA. Inhibition of rat respiratory-tract cytochrome P- 450 activity after acute low-level m-xylene inhalation: role in 1-nitro-naphthalene toxicity. Inhal Toxicol 2004;16(3):125-132   DOI   ScienceOn
12 Kataoka H, Saito K. Recent advances in SPME techniques in biomedical analysis. J Pharmaceutical and Biomedical Analysis 2011;54:926-950   DOI   ScienceOn
13 Lauwerys RR, Hoet P. Industrial Chemical Exposure: Guidelines for Biological Monitoring. 2nd ed., Lewis Publishers, Florida, 1993, 1-13
14 LoPachin RM, Jones RC, Patterson TA, Slikker WJr, Barber DS. Application of proteomics to the study of molecular mechanisms in neurotoxicology. Neurotoxicology 2003;24(6) :761-775   DOI   ScienceOn
15 Lu Y, Cederbaum AI. CYP2E1 and oxidative liver injury by alcohol. Free Radical Biology & Medicine 2008;44:723-738   DOI   ScienceOn
16 Nelson DR, Zeldin DC, Hoffman SM, Maltais LJ, Wain HM, Nebert DW. Comparison of cytochrome P450 (CYP) genes from the mouse and human genomes, including nomenclature recommendations for genes, pseudogenes and alternative-splice variants. Pharmacogenetics 2004;14(1):1-18
17 NIOSH. NIOSH manual of analytical methods, 4th ed., Method 1051, U.S. Department of Health and Human Services, Cincinnati 1996
18 Ogata M, Sugihara R, Kira S. Quantitative determination of urinary HA and m- or p-xylene exposure by HPLC. Int Arch Occup Environ Health 1977;39:199-206   DOI   ScienceOn
19 Omura T, Ishimura Y, Fujii-Kuriyama Y. Cytochrome P-450. 2nd ed., Kodansha Ltd, Tokyo, 1993
20 Razavi M, Pope ME, Soste MV, Eyford BA, Jackson AM, Anderson NL, Pearson TW. MALDI immunoscreening (MiSCREEN): A method for selection of anti-peptide monoclonal antibodies for use in immunoproteomics. J Immunological Methods 2011;364:50-64   DOI
21 Stierum R, Heijne W, Kienhuis A, Ommen BV, Groten J. Toxicogenomics concepts and applications to study hepatic effects of food additives and chemicals. Toxicol Appl Pharmacol 2005;207:S179-S188