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http://dx.doi.org/10.21289/KSIC.2022.25.3.365

Comparison of Scattered Light of ex vivo Mouse Neutrophils by Different Wavelength Laser Irradiation  

Park, Jae-Sung (Graduated School, Department of Mechnical Engineer, Kyungpook National University)
Son, Min-Ji (Laser Application Center, Kyungpook National University)
Hwang, Chang-Soon (School of Mechnical Engineering Kyungpook National University)
Lee, Ho (Laser Application Center, Kyungpook National University)
Publication Information
Journal of the Korean Society of Industry Convergence / v.25, no.3, 2022 , pp. 365-378 More about this Journal
Abstract
Complete blood cell count(CBC) is a technique that counts leukocytes for each type of blood cell being analyzed. The principle is that laser is incident to ex vivo flowing leukocytes in a microcapillary tube and scattered light occurs by laser and leukocytes. By collecting the scattered light, we can count the types of cells because different cells generate different light-scattering patterns. However, the technique has an intrinsic limitation, scattering pattern is shown in a wide range region in the resulting, which makes it difficult to accurate analyze and use fluorescent dyes. To overcome this limitation, a new design of CBC with a dual laser, which irradiates with orthogonal angles for collecting quad-scattering information was proposed. Before development, the scattering difference depending on wavelength must be investigated to only catch up to the scattered signal by angles. Some studies, which focused on simple particles, have been conducted to theoretically and experimentally investigate different scatterings by wavelength. In this study, we propose an optical system for measuring scattered light and investigate a complex particle. As a result, the green laser made strong scattering signals in both the forward and side direction: 10% and 30%, respectively.
Keywords
Scattering; Neutrophil; Complete Blood Cell Count(CBC); Amsterdam Discrete Dipole Approximation(ADDA);
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1 M. A. Yurkin, et al., "Experimental and theoretical study of light scattering by individual mature red blood cells by use of scanning flow cytometry and a discrete dipole approximation," 44, 5249-5256 (2005).   DOI
2 E. M. Purcell, and C. R. Pennypacker, "Scattering and absorption of light by nonspherical dielectric grains," The Astrophysical Journal 186, 10 (1973).
3 A. G. Hoekstra, et al., "Radiation forces in the discrete-dipole approximation," Journal of the Optical Society of America A 18, 10 (2001).
4 YURKIN, Maxim A.; HOEKSTRA, Alfons G. The discrete-dipole-approximation code ADDA: capabilities and known limitations. Journal of Quantitative Spectroscopy and Radiative Transfer, 2011, 112.13: 2234-2247.   DOI
5 F. Grasser, A. D'Arrigo, and S. Colombi, "JOE: A mobile,inverted pendulum," IEEE Trans. on Industrial Electronics, vol.49, no. 1, pp. 107-114, (2002).   DOI
6 한경자, 이원배, "다수의 레이저를 사용하는 세포분석 장치" 대한민국 특허, 10-1681422, (2016).
7 E. S. Yastrebova, et al., "Dual-wavelength angle-resolved light scattering used in the analysis of particles by scanning flow cytometry," Journal of Optics 23, 10 (2021).
8 M. A. Ansari, and E. Mohajerani, "Mechanisms of laser-tissue interaction: I. optical properties of tissue," Journal of Lasers in Medical Sciences 2, 7 (2011).
9 H. C. v. d. Hulst, "Light Scattering by Small particles," Dover Publications (1982).
10 V. Ost, et al., "Flow cytometric differentiation of erythrocytes and leukocytes in dilute whole blood by light scattering," 32, 191-197 (1998).   DOI
11 M. A. Yurkin, and A. G. Hoekstra, "The discrete dipole approximation: An overview and recent developments," Journal of Quantitative Spectroscopy & Radiative Transfer 106, 35 (2007).
12 Y. W. Wang, et al., "Light scattering models of white blood cells and back-scattering distribution analysis of them," Opt Appl 41, 527-539 (2011).
13 P. M. Sloot, et al., "Scattering matrix elements of biological particles measured in a flow through system: theory and practice," 28, 1752-1762 (1989).   DOI
14 D. Y. Orlova, et al., "Light scattering by neutrophils: model, simulation, and experiment," J Biomed Opt 13, 054057 (2008).   DOI
15 A. G. Hoekstra, and P. M. A. Sloot, "New Computational Techniques to Simulate Light Scattering from Arbitrary Particles," Particle & Particle System Characterization 11, 5 (1994).
16 A. G. Hoekstra, and P. M. A. Sloot, "Coupled Dipole Simulations of Elastic Light Scattering on Parallel Systems," International Journal of Modern Physics C 6.05 (1995).
17 BOSSCHAART, Nienke, et al. Measurements of wavelength dependent scattering and backscattering coefficients by low-coherence spectroscopy. Journal of biomedical optics, 2011, 16.3: 030503.   DOI
18 이호, 박재성, 한경자 "혈구 분석 장치, 이를 이용한 혈구 분석 방법" 대한민국 특허 10-2122020 (2018).
19 LAVEN, Philip. MiePlot (A computer program for scattering of light from a sphere using Mie theory & the Debye series). http://www.philiplaven.com/mieplot.htm, 2011.
20 V. P. Maltsev, et al., "Optics of white blood cells: optical models, simulations, and experiments," (2011).