Browse > Article
http://dx.doi.org/10.12772/TSE.2019.56.125

Evaluation of Polyvinylidene Fluoride and Polyacrylonitrile Nanofibers for Barrier Membrane Application in Guided Bone Regeneration  

Lim, Yun Kyong (Department of Oral Biochemistry, School of Dentistry, Chosun University)
Kook, Joong Ki (Department of Oral Biochemistry, School of Dentistry, Chosun University)
Yu, Sang Joun (Department of Periodontology, School of Dentistry, Chosun University)
Lee, Won Pyo (Department of Periodontology, School of Dentistry, Chosun University)
Lee, Kyung Hyun (Department of Periodontology, School of Dentistry, Chosun University)
Kim, Hee Jung (Department of Prosthodontics, School of Dentistry, Chosun University)
Lee, Seung Hoon (Department of Organic Materials and Fiber Engineering, Soongsil University)
Kim, Chan (Amogreentech Co., Ltd.)
Publication Information
Textile Science and Engineering / v.56, no.3, 2019 , pp. 125-134 More about this Journal
Abstract
Recently, various nanofiber membranes for guided bone regeneration using electrospinning methods have been reported. The purpose of this study was to evaluate the in vitro osteogenesis efficiency of electrospun polyvinylidene fluoride (PVDF) and polyacrylonitrile (PAN) membranes. Nano sized topography, mechanical properties, and biological evaluations were performed to compare the PVDF and PAN nanofiber membranes with the commercially available polytetrafluoroethylene (PTFE) membrane. The cytotoxicities of these membranes were evaluated using the MTT assay. Alkaline phosphatase (ALP) activity and the calcium concentration were determined to evaluate the in vitro bone formation activities of the above-mentioned membranes. Expression levels of the osteoblast differentiation marker genes (RUNX2, OSX, OCN, and COL1A1 genes) were determined using the real-time quantitative polymerase chain reaction (RT-qPCR) and enzyme-linked immunosorbent (ELISA) assay. Approximately 300 nm three-dimensional porous structures, with pores connected/extending from the surface to the rear/bottom were observed in each PVDF and PAN nanofiber membrane using a scanning electron microscope. The strength and elongation of the porous structures were superior to those seen in the PTFE membrane. The cell viability, ALP activity, and calcium concentration were higher in these nanofiber membranes as compared to those in the PTFE membrane. RT-qPCR and ELISA assay analysis also showed that the nanofiber membranes had better osteogenesis efficiencies as compared to the PTFE membrane after 7 days. The results in this study demonstrate that the electrospun PVDF and PAN nanofiber membranes could potentially function as barrier membranes for bone tissue regeneration.
Keywords
bone regeneration; electrospinning; nanofibers; polyacrylonitrile; polyvinylidene fluoride; PTFE;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 G. I. Im, "Intracellular Signal Transduction Pathways and Transcription Factors for Osteogenesis", J. Rheum. Dis.(JRD), 2008, 15, 1-10.
2 Y. Liu, C. Xu, Y. Gu, X. Shen, Y. Zhang, B. Li, and L. Chen, "Polydopamine-modified Poly(L-lactic acid) Nanofiber Scaffolds Immobilized with an Osteogenic Growth Peptide for Bone Tissue Regeneration", RSC Adv., 2019, 9, 11722-11736.   DOI
3 J. H. Kim, D. K. Kim, O. J. Lee, H. W. Ju, J. M. Lee, B. M. Moon, H. J. Park, D. W. Kim, J. M. Lee, and C. H. Park, "Osteoinductive Silk Fibroin/titanium Dioxide/hydroxyapatite Hybrid Scaffold for Bone Tissue Engineering", Inter. J. Biol. Macromol., 2016, 82, 160-167.   DOI
4 K. Nakashima, X. Zhou, G. Kunkel, Z. Zhang, J. M. Deng, R. R. Behringer, and B. Crombrugghe, "The Novel Zinc Fingercontaining Transcription Factor Osterix is Required for Osteoblast Differentiation and Bone Formation", Cell, 2002, 108, 17-29.   DOI
5 X. Wang, B. Ding, and B. Li, "Biomimetic Electrospun Nanofibrousstructures for Tissue Engineering", Materials Today, 2013, 16, 229-241.   DOI
6 S. H. Lee, Y. M. So, S. H. Jang, H. J. Sim, and C. Kim, "A Feasibility Study of Commercialization of PVDF Nanofiber Containing Plasticizer", Text. Sci. Eng., 2017, 54, 217-223.   DOI
7 C. S. Shin and H. Y. Cho, "Bone Remodeling and Mineralization", Endocrinol. Metab., 2005, 20, 543-555.
8 Y. S. Won, C. H. Choi, and H. N. OH, "Risk Factors of Periodontal Disease in Korean Adults", J. Korean Acad. Oral Health, 2014, 38, 176-183.   DOI
9 A. H. Melcher, "On the Repair Potential of Periodontal Tissue", J. Periodontol., 1976, 47, 256-260.   DOI
10 D. H. Han, K. S. Hong, C. H. Chung, and S. B. Yim, "A Comparative Study for Guided Bone Regeneration of Silk Fibroin Nanomembrane(NanoGide-STM)", The Korean Academy of Periodontology, 2008, 38, 475-482.   DOI
11 J. Gottlow, S. Nyman, J. Lindhe, T. Karring, and J. Wennstrom, "New Attachment Formation in the Human Periodontium by Guided Tissue Regeneration. Case Reports", J. Clin. Periodontol., 1986, 13, 604-616.   DOI
12 S. Nyman, T. Karring, and J. Lindhe, "The Regenerative Potential of the Periodontal Ligament. An Experimental Study in the Monkey", J. Clin. Periodontol., 1982, 9, 257-265.   DOI
13 Z. Sheikh, M. N. Abdallah, N. Hamdan, M. A. Javaid, and Z. Khurshid, "Barrier Membranes for Periodontal Guided Tissue Regeneration Applications", Handbook of Oral Biomaterials, 1st ed., Chapter 19, Pan Stanford Publishing, 2014, pp.601-638.
14 R. K. Schenk, D. Buser, W. R. Hardwick, and C. Dahlin, "Healing Pattern of Bone Regeneration in Membraneprotected Defects: A Histologic Study in the Canine Mandible", Int. J. Oral. Maxillofac Implants, 1994, 9, 13-29.
15 E. G. Cho, C. Kim, J. K. Kook, Y. I. Jeong, J. H. Kim, Y. A. Kim, M. Endo, and C. H. Hwang, "Fabrication of Electrospun PVDF Nanofiber Membrane for Western Blot with High Sensitivity", J. Membr. Sci., 2012, 389, 349-354.   DOI
16 J. Xue, M. He, Y. Liang, A. Crawford, P. Coates, D. Chen, R. Shi, and L. Zhang, "Fabrication and Evaluation of Electrospun PCL-gelatin Micro-/nanofiber Membranes for Anti-infective GTR Implants", J. Mat. Chem. B, 2014, 2, 6867-6877.   DOI
17 H. G. Jeong, Y. S. Han, K. H. Jung, and Y. J. Kim, "Poly(vinylidene fluoride) Composite Nanofibers Containing Polyhedral Oligomeric Silsesquioxane-Epigallocatechin Gallate Conjugate for Bone Tissue Regeneration", Nanomaterials, 2019, 9, 184-199.   DOI
18 H. Wei, M. Zuwei, Y. Thomas, E. T. Wee, and R. Seeram, "Fabrication of Collagen-coated Biodegradable Polymer Nanofiber Mesh and Its Potential for Endothelial Cells Growth", Biomaterials, 2005, 26, 7606-7615.   DOI
19 J. Y. Shim, I. H. Kim, M. K. Paik, and A. S. Om, "Proliferation and Extra Cellular Matrix Formation Effects of Genistein on Human Osteoblast-like MG-63 Cell Line", Cancer Prev. Res., 2009, 14, 48-53.
20 B. R. Sin, H. M. Kim, S. M. Kim, D. K. Kim, J. E. Song, C. H. Park, and G. S. Kang, "Osteogenesis Differentiation of Rabbit Bone Marrow-mesenchymal Stem Cells in Silk Scaffold Loaded with Various Ratios of Hydroxyapatite", Polymer(Korean), 2016, 40, 915-924.   DOI