Browse > Article
http://dx.doi.org/10.12772/TSE.2018.55.286

Physical and Biological Properties of Bio-membranes Obtained by Using PVA and Sulfonated Polymers  

Kim, Min Gu (Department of Physical Therapy, Eulji University)
Lee, Dong Keon (Department of Advanced Materials and Chemical Engineering, Hannam University)
Kwon, Hyuck Joon (Department of Physical Therapy, Eulji University)
Na, Yang Ho (Department of Advanced Materials and Chemical Engineering, Hannam University)
Publication Information
Textile Science and Engineering / v.55, no.4, 2018 , pp. 286-294 More about this Journal
Abstract
We prepared bio-membranes by mixing polyvinyl alcohol (PVA) with a natural polymer [${\kappa}$-Carrageenan (${\kappa}-Car$) or chondroitin sulfate (CS)] having a sulfonate group similar to proteoglycan of articular cartilage and investigated the mechanical, thermal, and surface properties of these bio-membranes that varied with the content ratio of the natural polymer. We found that the mechanical properties of the bio-membranes decreased with the ratio of ${\kappa}-Car$ or CS. It was found that the mesenchymal stem cells (MSCs) virtually did not adhere to the PVA hydrogel film prepared by freeze-thawing method, whereas they adhered to the PVA hydrogel film prepared by solution casting. In addition, the adhesion rate of the MSCs on the bio-membranes gradually increased with the ratio of ${\kappa}-Car$ or CS.
Keywords
hydrogel; cartilage; bio-membrane; microfracture; polyvinyl alcohol;
Citations & Related Records
연도 인용수 순위
  • Reference
1 J. R. Steadman, W. G. Rodkey, and J. J. Rodrigo, "Microfracture: Surgical Technique and Rehabilitation to Treat Chondral Defects", Clin. Orthop. Relat. Res., 2001, 391, 362-369.   DOI
2 B. R. Mandelbaum, J. E. Browne, F. Fu, L. Micheli, J. B. Mosely, C. Erggelet, T. Minas, and L. Peterson, "Articular Cartilage Lesions of the Knee", Am. J. Sports Med., 1998, 26, 853-861.   DOI
3 G. D. Smith, G. Knutsen, and J. B. Richardson, "A Clinical Review of Cartilage Repair Techniques", J. Bone. Joint. Surg. Br., 2005, 87, 445-449.
4 K. B. L. Lee, J. H. P. Hui, I. C. Song, L. Ardany, and E. H. Lee, "Injectable Mesenchymal Stem Cell Therapy for Large Cartilage Defects - a Porcine Model", Stem. Cells, 2007, 25, 2964-2971.   DOI
5 F. Shapiro, S. Koide, and M. J. Glimcher, “Cell Origin and Differentiation in the Repair of Full-thickness Defects of Articular Cartilage”, JBJS, 1993, 75, 532-553.   DOI
6 J. Y. Chung, D. H. Lee, T. H. Kim, K. S. Kwack, K. H. Yoon, and B. H. Min, "Cartilage Extra-cellular Matrix Biomembrane for the Enhancement of Microfractured Defects", Knee Surg. Sports Traumatol. Arthrosc., 2014, 22, 1249-1259.   DOI
7 B. H. Min, S. R. Park, and B. H. Choi, "Method for Preparing a Cell-Derived ExtracellularMatrix Membrane", KR Patent, 1015397000000 (2008).
8 J. S. Choi and B. S. Kim, "Bilayer Film Consisting of ECM and Biocompatible Polymer and Method for Manufacturing", KR Patent, 1011023080000 (2010).
9 J. Y. Jang and H. J. Kim, "Method of Preparing Membrane for Leading Regeneration Produced from Material Derived from Biological Tissue", KR Patent, 1014340410000 (2014).
10 H. J. Kwon, K. Yasuda, Y. Ohmiya, K. I. Honma, Y. M. Chen, and J. P. Gong, “In vitro Differentiation of Chondrogenic ATDC5 Cells is Enhanced by Culturing on Synthetic Hydrogels with Various Charge Densities”, Acta Biomater., 2010, 6, 494-501.   DOI
11 K. Yasuda, N. Kitamura, J. P. Gong, K. Arakaki, H. J. Kwon, S. Onodera, Y. M. Chen, T. Kurokawa, Y. Ohmiya, and Y. Osada, "A Novel Double‐network Hydrogel Induces Spontaneous Articular Cartilage Regeneration in vivo in a Large Osteochondral Defect", Macromol. Biosci., 2009, 9, 307-316.   DOI
12 E. Otsuka and A. Suzuki, "A Simple Method to Obtain a Swollen PVA Gel Crosslinked by Hydrogen Bonds", J. Appl. Polym. Sci., 2009, 114, 10-16.   DOI
13 S. M. Pawde and K. Deshmukh, "Characterization of Polyvinyl Alcohol/gelatin Blend Hydrogel Films for Biomedical Applications", J. Appl. Polym. Sci., 2008, 109, 3431-3437.   DOI
14 J. S. Park, J. W. Park, and E. Ruckenstein, “Thermal and Dynamic Mechanical Analysis of PVA/MC Blend Hydrogels”, Polymer, 2001, 42, 4271-4280.   DOI
15 Y. Zhang, L. Ye, M. Cui, B. Yang, J. Li, H. Sun, and F. Yao, "Physically Crosslinked Poly(vinyl alcohol)-Carrageenan Composite Hydrogels: Pore Structure Stability and Cell Adhesive Ability", RSC Adv., 2015, 5, 78180-78191.   DOI
16 P. Chopra, "Development of PVA-Carrageenan Based Scaffolds and Evaluating Its Efficacy on Osteosarcoma Upon Cryopreservation", Master's Thesis, Thapar University, Punjab, India, 2015.
17 Y. M. Chen, N. Shiraishi, H. Satokawa, A. Kakugo, T. Narita, J. P. Gong, and J. Ando, “Cultivation of Endothelial Cells on Adhesive Protein-free Synthetic Polymer Gels”, Biomaterials, 2005, 26, 4588-4596.   DOI
18 C. T. Lee, P. H. Kung, and Y. D. Lee, "Preparation of Poly(vinyl alcohol)-Chondroitin Sulfate Hydrogel as Matrices in Tissue Engineering", Carbohydr. Polym., 2005, 61, 348-354.   DOI
19 T. Fukumori and T. Nakaoki, "Significant Improvement of Mechanical Properties for Polyvinyl Alcohol Film Prepared from Freeze/thaw Cycled Gel", Open J. Org. Polym. Mater., 2013, 3, 110-116.   DOI
20 M. F. Pittenger, A. M. Mackay, S. C. Beck, R. K. Jaiswal, R. Douglas, J. D. Mosca, M. A. Moorman, D. W. Simonetti, S. Craig, and D. R. Marshak, “Multilineage Potential of Adult Human Mesenchymal Stem Cells”, Science, 1999, 284, 143-147.   DOI
21 R. McBeath, D. M. Pirone, C. M. Nelson, K. Bhadriraju, and C. S. Chen, "Cell Shape, Cytoskeletal Tension, and RhoA Regulate Stem Cell Lineage Commitment", Dev. Cell., 2004, 6, 483-495.   DOI
22 L. Gao, R. McBeath, and C. S. Chen, “Stem Cell Shape Regulates a Chondrogenic Versus Myogenic Fate through Rac1 and N-cadherin”, Stem Cells, 2010, 28, 564-572.