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http://dx.doi.org/10.12772/TSE.2015.52.373

A Protein Sensory System Comprising a Fluorescent Conjugated Polyelectrolyte and DNA Aptamer  

Son, Jihye (Department of Advanced Organic Materials and Textile System Engineering, Chungnam National University)
Kim, Choongho (Department of Advanced Organic Materials and Textile System Engineering, Chungnam National University)
Lee, Taek Seung (Department of Advanced Organic Materials and Textile System Engineering, Chungnam National University)
Publication Information
Textile Science and Engineering / v.52, no.6, 2015 , pp. 373-378 More about this Journal
Abstract
A water-soluble anionic conjugated polyelectrolyte was successfully synthesized via the Suzuki cross-coupling reaction in the presence of a palladium catalyst. Using this fluorescent polyelectrolyte, a new concept for a rapid, label-free lysozyme-sensing method is proposed via possible naked-eye detection of the emission color change. Intermolecular exciton migration in the conjugated polyelectrolyte-based complex was adopted to enhance the selectivity and sensitivity for lysozyme sensing by the formation and dissociation of the polymer-lysozyme assay complex in the absence and presence of the anti-lysozyme aptamer, respectively. The polymer-lysozyme complex showed red emission because of cooperative aggregation of the conjugated polyelectrolyte and lysozyme. Upon exposure to the aptamer, the complex dissociated into individual molecules, resulting in a transparent blue-emitting solution. This occurred because lysozyme is released from the complex by the aptamer via the more favorable binding between the two molecules (lysozyme and aptamer).
Keywords
conjugated polyelectrolyte; sensors; fluorescence; lysozyme; emission color change; aptamer;
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