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http://dx.doi.org/10.5933/JKAPD.2020.47.2.120

Antimicrobial Effect of Photodynamic Therapy Using Plaque Disclosing Agent  

Kho, Junhee (Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry, Gangneung-Wonju National University)
Park, Howon (Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry, Gangneung-Wonju National University)
Lee, Juhyun (Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry, Gangneung-Wonju National University)
Seo, Hyunwoo (Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry, Gangneung-Wonju National University)
Lee, Siyoung (Department of Microbiology and Immunology, College of Dentistry, Gangneung-Wonju National University)
Publication Information
Journal of the korean academy of Pediatric Dentistry / v.47, no.2, 2020 , pp. 120-127 More about this Journal
Abstract
The aim of this study was to evaluate the antimicrobial effect of photodynamic therapy (PDT) using plaque disclosing agent, 10 - 20 mM erythrosine, as a photosensitizer. Multispecies cariogenic biofilms containing Streptococcus mutans, Lactobacillus casei and Candida albicans were formed on hydroxyapatite disc. 20 μM, 10 mM and 20 mM erythrosine were applied as a photosensitizer for 3 minutes, and then light-emitting diode (LED) irradiated for 24 seconds. Colony-forming unit (CFU) were measured and biofilms were observed using confocal laser scanning microscopy (CLSM). CFU were significantly decreased in the PDT groups using 10 - 20 mM erythrosine (10 mM, 20mM) and the results were also confirmed by CLSM. This study confirms the high antimicrobial effect of photodynamic therapy using plaque disclosing agent as a photosensitizer.
Keywords
Photodynamic therapy; Erythrosine; Plaque disclosing agent; Multispecies biofilm;
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Times Cited By KSCI : 4  (Citation Analysis)
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