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http://dx.doi.org/10.5933/JKAPD.2019.46.1.85

Cytotoxicity of Various Calcium Silicate-based Materials with Stem Cells from Deciduous Teeth  

Yun, Jihye (Department of Pediatric Dentistry, College of Dentistry, Wonkwang University)
You, Yong-Ouk (Department of Oral Biochemistry, College of Dentistry, Wonkwang University)
Ahn, Eunsuk (Department of Dental Hygiene, Kyungbok University)
Lee, Jun (Department of Oral and Maxillofacial Surgery, College of Dentistry, Wonkwang University)
An, So-Youn (Department of Pediatric Dentistry, College of Dentistry, Wonkwang University)
Publication Information
Journal of the korean academy of Pediatric Dentistry / v.46, no.1, 2019 , pp. 85-92 More about this Journal
Abstract
The purpose of this study was to compare and evaluate the cytotoxicity of 3 calcium silicate-based materials (CSMs) on stem cells from human exfoliated deciduous teeth (SHEDs). The powder of Retro $MTA^{(R)}$ (RM), $EZ-Seal^{TM}$ (EZ) and ENDOCEM $Zr^{(R)}$ (EN) was eluted with SHED culture media and then filtered. The SHEDs were cultured in the presence of the various concentrations of the eluate. To investigate the effect of the 3 CSMs on SHED proliferation, the MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay was performed. Flow cytometry analysis was also performed to identify any changes in the cellular phenotype. The absorbance values of the SHEDs cultured in the eluate of samples at a 10% concentration showed the following relation: RM > EN > EZ (p = 0.0439). However, the SHEDs maintained their mesenchymal phenotype regardless of product exposure. Although the 3 CSMs did not alter the SHED stem cell markers, EZ may be a less cytocompatible than RM and EN.
Keywords
Cytotoxicity; Calcium silicate; Mineral trioxide aggregate; Deciduous tooth; Stem cells;
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