Browse > Article
http://dx.doi.org/10.5933/JKAPD.2018.45.2.144

Roles of Sonic Hedgehog Signaling During Tooth Root and Periodontium Formation  

Hwang, Jaewon (Cluster for Craniofacial Development and Regeneration Research, Institute of Oral Bioscience, School of Dentistry, Chonbuk National University)
Cho, Eui-sic (Cluster for Craniofacial Development and Regeneration Research, Institute of Oral Bioscience, School of Dentistry, Chonbuk National University)
Yang, Yeonmi (Department of Pediatric Dentistry and Institute of Oral Bioscience, School of Dentistry, Chonbuk National University)
Publication Information
Journal of the korean academy of Pediatric Dentistry / v.45, no.2, 2018 , pp. 144-153 More about this Journal
Abstract
The aim of this study was to understand the roles of Sonic Hedgehog (SHH) signaling during tooth root and periodontium formation. In this study, we generated the dental mesenchyme-specific Smoothened (Smo) activated/inactivated mice with the activity of Cre recombinase under the control of osteocalcin promoter. In the Smo activated mutant molar sections at the postnatal 28 days, we found extremely thin root dentin and widened pulp chamber. Picrosirius red staining showed loosely arranged fibers in the periodontal space and decreased cellular cementum with some root resorption. Immunohistochemical staining showed less localization of matrix proteins such as Bsp, Dmp1, Pstn, and Ank in the cementum, periodontal ligament, and/or cementoblast. In the Smo inactivated mutant mouse, there was not any remarkable differences in the localization of these matrix proteins compared with the wild type. These findings suggest that adequate suppressing regulation of SHH signaling is required in the development of tooth root and periodontium.
Keywords
Sonic Hedgehog signaling; Smoothened; Periodontium;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Cohen MM Jr : The hedgehog signaling network. Am J Med Genet, 123:5-28, 2003.
2 Ingham PW, McMahon AP : Hedgehog signaling in animal development: paradigms and principles. Gene Dev, 15:3059-3087, 2001.   DOI
3 Khan M, Seppala M, Cobourne MT, et al. : Hedgehog pathway gene expression during early development of the molar tooth root in the mouse. Gene Expr Patterns, 7:239-243, 2007.   DOI
4 Huang XF, Xu X, Chai Y, et al. : Smad4-Shh-Nfic signaling cascade-mediated epithelial- mesenchymal interaction is crucial in regulationg tooth root development. J Bone Miner Res, 25:1167-1178, 2010.
5 Nakatomi M, Morita I, Ota MS, et al. : Sonic Hedgehog signaling is important in tooth root development. J Dent Res, 85:427-431, 2006.   DOI
6 Kim TH, Bae CH, Cho ES, et al. : Nfic regulates tooth root patterning and growth. Anat Cell Biol, 48:188-194, 2015.   DOI
7 Zhang XM, Ramalho-Santos M, McMahon AP : Smoothened mutants reveal redundant roles for Shh and Ihh signaling including regulation of L/R asymmetry by the mouse node. Cell, 105:781-792, 2001.   DOI
8 Jeong J, Mao J, McMahon AP, et al. : Hedgehog signaling in the neural crest cells regulates the patterning and growth of facial primordia. Genes Dev, 18:937-951, 2004.   DOI
9 Kim TH, Lee JY, Cho ES, et al. : Constitutive stabilization of ${\beta}$-catenin in the dental mesenchyme leads to excessive dentin and cementum formation. Biochem Biophys Res Commun, 412:549-555, 2011.   DOI
10 Kim TH, Bae CH, Cho ES, et al. : ${\beta}$-catenin is required in odontoblasts for tooth root formation. J Dent Res, 92:215-221, 2013.   DOI
11 Martinez C, Smith PC, Palma V, et al. : Sonic Hedgehog stimulates proliferation of human periodontal ligament stem cells. J Dent Res, 90:483-488, 2011.   DOI
12 Thesleff I : The genetic basis of tooth development and dental defects. Am J Med Genet, 140:2530-2535, 2006.
13 Long F, Zhang XM, McMahon AP, et al. : Genetic maniulation of hedgehog signaling in the endochondral skeleton reveals a direct role in the regulation of chondrocyte proliferation. Development, 128:5099-5108, 2001.
14 Tan X, Weng T, Yang X, et al. : Smad4 is required for maintaining normal murine postnatal bone homeostasis. J Cell Sci, 120:2162-2170, 2007.   DOI
15 Hardcastle Z, Mo R, Sharpe PT, et al. : The SHH signalling pathway in tooth development: defects in Gli2 and Gli3 mutants. Development, 125:2803-2811, 1998.
16 Slavkin HC, Boyde A : Cementum: an epithelial secretory product? (abstract). J Dent Res, 53:157, 1975.
17 Hammarstrom L : Enamel matrix, cementum development and regeneration. J Clin Periodontol, 24:658-668, 1997.   DOI
18 Aukhil I, Nishimura K, Fernyhough W : Experimental regeneration of the periodontium. Crit Rev Oral Biol Med, 1:101-115, 1990.   DOI
19 Huang X, Bringas P Jr, Chai Y, et al. : Fate of HERS during tooth root development. Dev Biol, 334:22-30, 2009.   DOI
20 Gritli-Linde A, Bei M, McMahon AP, et al. : Shh signaling within the dental epithelium is necessary for cell proliferation, growth and polarization. Development, 129:5323-5337, 2002.   DOI
21 Lim WH, Liu B, Cheng D, et al. : Down regulation of Wnt causes root resorption. Am J Orthod Dentofacial Orthop, 146:337-45, 2014.   DOI
22 Bae WJ, Auh QS, Lim HC, et al. : Sonic Hedgehog Promotes Cementoblastic Differentiation via Activating the BMP Pathways. Calcif Tissue Int, 99:396-407, 2016.   DOI