Browse > Article
http://dx.doi.org/10.5933/JKAPD.2017.44.2.170

Comparison of Cariogenicity of Bovine Milk and Low-fat Milk on Streptococcus mutans Biofilm  

Hwang, Minseon (Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry, Gangneung-Wonju National University)
Park, Howon (Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry, Gangneung-Wonju National University)
Lee, Juhyun (Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry, Gangneung-Wonju National University)
Seo, Hyunwoo (Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry, Gangneung-Wonju National University)
Lee, Siyoung (Department of Oral Microbiology, Oral Science Research Center, College of Dentistry, Gangneung-Wonju National University)
Publication Information
Journal of the korean academy of Pediatric Dentistry / v.44, no.2, 2017 , pp. 170-179 More about this Journal
Abstract
The purpose of this study was to compare the cariogenicity of commercially available bovine milk and low-fat milk in a biofilm model using the CDC Biofilm Reactor. Streptococcus mutans ATCC 25175 biofilms were formed on saliva coated bovine enamel slabs in a CDC Biofilm Reactor. Biofilms were exposed three times per day to one of the following materials: commercial whole milk (fat content: 3.4%), low-fat milk (fat content: 1%), or 0.9% NaCl. Medium pH was measured at different time points. After 5 days, biofilms were separated from slabs to evaluate the CFUs. The biofilm thickness was observed by confocal laser-scanning microscopy (CLSM). Enamel slab's demineralization was assessed by measuring surface microhardness before and after the experiment. For microhardness and CFUs assessment, no significant difference was found among the three groups. All groups showed similar pattern of medium pH change and biofilm thickness. Our results showed that there was no difference in the cariogenicity between whole milk and low-fat milk. Both milks were relatively non-cariogenic compared to the control group.
Keywords
Cariogenic; Milk; Fat; Streptococcus mutans; Biofilm;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Ministry of Health & Welfare : 2012 Korea National Oral Health Survey, 2012.
2 Ministry of Health & Welfare, Korea Centers for Disease Control and Prevention : Korea Health Statistics 2014, 2014.
3 Munoz-Sandoval C, Munoz-Cifuentes MJ, Giacaman RA, et al. : Effect of bovine milk on Streptococcus mutans biofilm cariogenic properties and enamel and dentin demineralization. Pediatr Dent, 34:197-201, 2012.
4 Takahashi N, Nyvad B : Caries ecology revisited: microbial dynamics and the caries process. Caries Res, 42:409-418, 2008.   DOI
5 Heo NR, Lee KH, An SY, et al. : Cariogenic Potential of Nutritional Supplements for Children on Bovine Teeth. J Korean Acad Pediatr Dent, 41:233-240, 2014.   DOI
6 Kim HK, Park HW, Shin IS, et al. : The antimicrobial effect of horseradish(armoracia rusticana) root extracts against streptococcus mutans isolated from human dental palque. J Korean Acad Pediatr Dent, 35:225-234, 2008.
7 Giacaman RA, Munoz MJ, Ccahuana-Vasquez RA, et al. : Effect of fluoridated milk on enamel and root dentin demineralization evaluated by a biofilm caries model. Caries Res, 46:460-466, 2012.   DOI
8 Kim JH, Lee YG, Ahn SH, et al. : Inhibition of glucan synthesis related gene expression of streptococcus mutans by xylitol treatment. J Korean Acad Pediatr Dent, 36:531-538, 2009.
9 Bowen WH, Lawrence RA : Comparison of the cariogenicity of cola, honey, cow milk, human milk, and sucrose. Pediatrics, 116:921-926, 2005.   DOI
10 Peres RC, Coppi LC, Franco EM, et al. : Cariogenicity of different types of milk: an experimental study using animal model. Braz Dent J, 13:27-32, 2002.
11 Grenby TH, Andrews AT, Mistry M, Williams RJ : Dental caries-protective agents in milk and milk products: investigations in vitro. J Dent, 29:83-92, 2001.   DOI
12 Sprong RC, Hulstein MF, Van der Meer R : Bactericidal activities of milk lipids. Antimicrob Agents Chemother, 45:1298-1301, 2001.   DOI
13 Huang CB, Ebersole JL : A novel bioactivity of omega-3 polyunsaturated fatty acids and their ester derivatives. Mol Oral Microbiol, 25:75-80, 2010.   DOI
14 Aimutis WR : Bioactive properties of milk proteins with particular focus on anticariogenesis. J Nutr, 134:989-995, 2004.   DOI
15 Mansson HL : Fatty acids in bovine milk fat. Food Nutr Res, 52, 2008.
16 Huang CB, George B, Ebersole JL : Antimicrobial activity of n-6, n-7 and n-9 fatty acids and their esters for oral microorganisms. Arch Oral Biol, 55:555-560, 2010.   DOI
17 Giacaman RA, Jobet-Vila P, Munoz-Sandoval C : Fatty acid effect on sucrose-induced enamel demineralization and cariogenicity of an experimental biofilm-caries model. Odontology, 103:169-176, 2015.   DOI
18 Giacaman RA, Munoz-Sandoval C : Cariogenicity of different commercially available bovine milk types in a biofilm caries model. Pediatr Dent, 36:1-6, 2014.
19 Mellberg JR, Petrou ID, Grote NE : The effect of plaque thickness on progression of artificial caries lesion formation in situ. J Dent Res, 69:865-867, 1990.   DOI
20 Zhou H, Weir MD, Antonucci JM, et al. : Evaluation of three-dimensional biofilms on antibacterial bonding agents containing novel quaternary ammonium methacrylates. International Journal of Oral Science, 6:77-86, 2014.   DOI
21 Petridou E, Athanassouli T, Panagopoulos H, Revinthi K : Sociodemographic and dietary factors in relation to dental health among Greek adolescents. Community Dent Oral Epidemiol, 24:307-311, 1996.   DOI
22 Desbois AP, Smith VJ : Antibacterial free fatty acids: activities, mechanisms of action and biotechnological potential. Appl Microbiol Biotechnol, 85:1629-1642, 2010.   DOI
23 Shetty V, Hegde AM, Nandan S, Shetty S : Caries protective agents in human milk and bovine milk: an in vitro study. J Clin Pediatr Dent, 35:389-392, 2011.   DOI
24 Palmer CA : Important relationships between diet, nutrition, and oral health. Nutr Clin Care, 4:4-14, 2001.   DOI
25 Kensche A, Reich M, Kummerer K, et al. : Lipids in preventive dentistry. Clin Oral Investig, 17:669-685, 2013.   DOI
26 Ministry of Health & Welfare : The Dietary Guidelines, 2016
27 Bylund G : Dairy processing handbook, 2nd ed., Tetra Pak Processing Systems AB Lund, 227-246, 2003.
28 Duarte PM, Reis AF, de Freitas PM, Ota-Tsuzuki C : Bacterial adhesion on smooth and rough titanium surfaces after treatment with different instruments. J Periodontol, 80:1824-1832, 2009.   DOI
29 Ccahuana-Vasquez RA, Cury JA : S. mutans biofilm model to evaluate antimicrobial substances and enamel demineralization. Braz Oral Res, 24:135-141, 2010.   DOI
30 An JS, Park HW, Seo HW, Lee SY : Antibacterial Properties of Pit and Fissure Sealant Containing S-PRG filler on Streptococcus mutans. J Korean Acad Pediatr Dent, 42:302-311, 2015.   DOI
31 Zero DT : In situ caries models. Adv Dent Res, 9:214-230, 1995.   DOI
32 Williams DL, Bloebaum RD : Observing the biofilm matrix of Staphylococcus epidermidis ATCC 35984 grown using the CDC biofilm reactor. Microsc Microanal, 16:143-152, 2010.   DOI
33 Petti S, Simonetti R, Simonetti D'Arca A : The effect of milk and sucrose consumption on caries in 6-to-11-year-old Italian schoolchildren. Eur J Epidemiol, 13:659-664, 1997.   DOI
34 Blanc V, Isabal S, Sanchez MC, et al. : Characterization and application of a flow system for in vitro multispecies oral biofilm formation. J Periodontal Res, 49:323-332, 2014.   DOI
35 Douterelo I, Sharpe RL, Boxall JB : Influence of hydraulic regimes on bacterial community structure and composition in an experimental drinking water distribution system. Water Res, 47:503-516, 2013.   DOI
36 Foka A, Katsikogianni MG, Anastassiou ED, et al. : The combined effect of surface chemistry and flow conditions on Staphylococcus epidermidis adhesion and ica operon expression. Eur Cell Mater, 24:386-402, 2012.   DOI
37 Weaver WM, Milisavljevic V, Miller JF, Di Carlo D : Fluid flow induces biofilm formation in Staphylococcus epidermidis polysaccharide intracellular adhesin-positive clinical isolates. Appl Environ Microbiol, 78:5890-5896, 2012.   DOI
38 Maezono H, Noiri Y, Asahi Y, et al. : Antibiofilm effects of azithromycin and erythromycin on Porphyromonas gingivalis. Antimicrob Agents Chemother, 55:5887-5892, 2011.   DOI
39 Stoodley P, Lewandowski Z, Boyle JD, Lappin-Scott HM : The formation of migratory ripples in a mixed species bacterial biofilm growing in turbulent flow. Environ Microbiol, 1:447-455, 1999.   DOI
40 Bowen WH : Food components and caries. Adv Dent Res, 8:215-220, 1994.   DOI