Browse > Article
http://dx.doi.org/10.5933/JKAPD.2015.42.2.151

Optimum Treatment Parameters for Photodynamic Antimicrobial Chemotherapy on Streptococcus mutans Biofilms  

Choi, Seojung (Department of Pediatric Dentistry, College of Dentistry, Gangneung-Wonju National University)
Park, Howon (Department of Pediatric Dentistry, College of Dentistry, Gangneung-Wonju National University)
Lee, Juhyun (Department of Pediatric Dentistry, College of Dentistry, Gangneung-Wonju National University)
Seo, Hyunwoo (Department of Pediatric Dentistry, College of Dentistry, Gangneung-Wonju National University)
Lee, Siyoung (Department of Microbiology and Immunology, Oral Science Research Center, College of Dentistry, Gangneung-Wonju National University)
Publication Information
Journal of the korean academy of Pediatric Dentistry / v.42, no.2, 2015 , pp. 151-157 More about this Journal
Abstract
The purpose of this study was to evaluate the effects of Photochemotherapy using a combination of erythrosine and standard halogen dental curing lights on the viability of Streptococcus mutans in the biofilm phase. To investigate the optimum treatment parameters, the researchers controlled the concentration of erythrosine, light irradiation time and the treatment time of erythrosine. The higher concentration of erythrosine (0, 10, 20, 40, 80 M) in the presence of light irradiation created greater effects in reducing the viability of S. mutans. The results showed a statistically significant difference among the antimicrobial effects in 20, 40, 80 M erythrosine. The higher irradiation time of light (0, 5, 15, 30, 60, 75s) in the presence of erythrosine showed greater effects in reducing the viability of S. mutans. There was statistically significant difference in 30, 60, 75 seconds. The higher treatment time of erythrosine (0, 1, 2.5, 5min) in the presence of erythrosine created greater effects on reduction of S. mutans viability. Statistically significant differences were found between 2.5 and 5 minutes of erythrosine treatment time. The results of this study showed that the photochemotherapy on S. mutans using erythrosine and the halogen dental curing lights conventionally used in dental clinics is effective in the condition of 20-40 M erythrosine concentration, irradiation time over 30 seconds, and erythrosine treatment time over 2.5 minutes.
Keywords
Photochemotherapy; Halogen Dental Curing Lights; Photosensitizing Agents; Erythrosine;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
연도 인용수 순위
1 Lee YH, Park HW, Lee JH, et al. : The photodynamic therapy on Streptococcus mutans biofilms using erythrosine and dental halogen curing unit. Int J Oral Sci, 4:196-201, 2012.   DOI
2 Park MS, Park HW : Susceptibility of Streptococcus mutans to photodynamic therapy with erythrosine and dental halogen curing unit. Gangneung: Gangneung-Wonju National University; 2011.
3 Jung JS, Park HW, Lee JH : The effect of photodynamic therapy on the viability of Streptococcus mutans isolated from oral cavity. J Korean Acad Pediatr Dent, 39:233-241, 2012.   DOI   ScienceOn
4 Dougherty TJ, Gomer CJ : Photodynamic therapy. J Natl Cancer Inst, 90:889-905, 1998.   DOI
5 Okamoto H, Iwase T : Dye-mediated bactercidal effect of He-Ne laser irradiation on oral microorganisms. Lasers Surg Med, 12:450-458, 1992.   DOI
6 Marsh PD, Bevis RA, Newman HN : Antibacterial activity of some plaque-disclosing agents and dyes. Caries Res, 23:348-350, 1989.   DOI
7 Metcalf D, Robinson C, Devine D, et al. : Enhancement of erythrosine-mediated photodynamic therapy of Streptococcous mutans biofilms by light fractionation. J Antimicrob Chemother, 58:190-192, 2006.   DOI
8 Wood S, Metcalf D : Erythrosine is a potential photosensitizer for the photodynamic therapy of oral plaque biofilms. J Antimicrob Chemother, 57:680-684, 2006.   DOI
9 Bomin Kwon, Ikhyun Bae, Shin Kim, et al. : Dental caries status of 14-16 year old adolescents in Yangsan area. J Korean Acad Pediatr Dent, 41:8-17, 2014.   DOI   ScienceOn
10 Loe H : Oral hygiene in the prevention of caries and periodental disease. Int Dent J, 50:129-139, 2000.   DOI   ScienceOn
11 Smith DJ : Dental caries vaccines: prospects and concerns. Crit Rev Oral Biol Med, 13:335-349, 2002.   DOI
12 Marsh PD : Microbiologic aspects of dental plague and dental caries. Dent Clin North Am, 43:599-614, 1999.
13 Francisco Ramos-Gomez, Young Jae Kim, Man-Wai Ng, et al. : New visions in pediatric dentistry keeping healthy teeth caries free: Pediatric cambra protocols. J Korean Acad Pediatr Dent, 40:72-82, 2013.   DOI
14 Hoyle BD, Costerton JW : Bacterial resistance to antibiotics : the role of biofilms. Prog Drug Res, 37:91-105, 1991.
15 Ka-Young Lee, Sang-Ho Lee, Nan-Young Lee : Evaluation of fluoride-releasing capacity from polyvinyl alcohol polymer tape supplemented with NaF in oral cavity. J Korean Acad Pediatr Dent, 40:89-97, 2013.   DOI   ScienceOn
16 Hellwig E, Lennon AM : Systemic versus topical fluoride. Caries Res, 38:258-262, 2004.   DOI
17 Nikolaos S. : Photodynamic therapy in the control of oral biofilms. Periodontology 2000, 55:143-166, 2011.   DOI
18 Jongcheol Park, Howon Park, Siyoung Lee : Enhancement of erythrosine photodynamic therapy against Streptococcus mutans by chlorhexidine. J Korean Acad Pediatr Dent, 40:241-246, 2013.   DOI
19 Wesley MS, Cynthia MA, Johan EL : Photodynamic therapeutics: basic principles and clinical applications. DDT, 4:507-517, 1999.   DOI
20 Konopka K, Goslinski T : Photodynamic therapy in dentistry. J Dent Res, 86:694-707, 2007.   DOI
21 Juliana YN : Antibacterial photodynamic therapy for dental caries : Evaluation of the photosensitizers used and light source properties. Photodiagnosis and Photodynamic Therapy, 9:112-131, 2012.
22 Zanin IC, Goncalves RB : Susceptibility of Streptococcus mutans biofilms to photodynamic therapy: an in vitro study. J Antimicrob Chemother 56, 56:324-330, 2005.   DOI
23 Chan Y, Lai CH : Bactericidl effects of different laser wavelengths on periodontopathic germs in photodynamic therapy. Lasers Med Sci, 18:51-55, 2003.   DOI
24 Dobson J, Wilson M : Sensitization of oral bacteria in biofilms to killing by light from a low-power laser. Arch Oral Biol, 37:883-887, 1992.   DOI
25 Giusti JS, Snatos PL : Antimicrobial photodynamic action on dentin using a light-emitting diode light source. Photomed Laser Surg, 26:281-287, 2008.   DOI
26 Costa AC, Chibebe JJ : Susceptibility of planktonic cultures of Streptococcus mutans to photodynamic therapy with a light-emitting diode. Braz Oral Res 24:413-418, 2010.   DOI