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http://dx.doi.org/10.5010/JPB.2022.49.3.257

Antimicrobial polyhydroxybutyrate submicron fiber mat loaded with extract of Hypericum perforatum  

Beran, Milos (Food Research Institute Prague)
Horna, Ales (Radanal Ltd.)
Vorisek, Viktor (Radanal Ltd.)
Berkova, Eliska (Food Research Institute Prague)
Korinkova, Radka (Centre of Organic Chemistry Ltd.)
Trousil, Vojtech (Centre of Organic Chemistry Ltd.)
Hrubanova, Marketa (Textile Testing Institute)
Publication Information
Journal of Plant Biotechnology / v.49, no.3, 2022 , pp. 257-270 More about this Journal
Abstract
The aim of this work was to prepare a new biodegradable polyhydroxybutyrate (PHB) submicron fiber mat loaded with hypericin-rich Hypericum perforatum raw extract by centrifugal spinning technology, an alternative approach to the traditional method of electrospinning to fabricate nanofibers or microfibers from solutions at high speed and low cost. Hypericins in methanol/acetone extract of H. perforatum were determined by UHPLC-MS/MS and HPLC/PDA. Submicron fiber mats composed of pure PHB or PHB enriched with H. perforatum extract were prepared using a pilot plant demonstrator for the centrifugal spinning technology and characterized by SEM. Singlet oxygen production was quantified by the 1,3-diphenylisobenzofuran (DPIBF) method in hexane. The results proved a significant production of singlet oxygen by the prepared submicron fiber mat. We also found a significant antibacterial activity against the bacterial strain Escherichia coli CCM 5417 by a method in accordance with JIS Z 2801/ISO 22196 standards. The H. perforatum extract-enriched PHB submicron fiber mats showed potential for the development of self-cleaning and antimicrobial air filters.
Keywords
Hypericum perforatum; Hypericin extract; Polyhydroxybutyrate; Submicron fiber; Singlet oxygen; Antimicrobial air filter;
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